2004
Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane
Vessey JP, Shi C, Jollimore CA, Stevens KT, Coca-Prados M, Barnes S, Kelly ME. Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane. Biochemistry And Cell Biology 2004, 82: 708-718. PMID: 15674438, DOI: 10.1139/o04-107.Peer-Reviewed Original ResearchConceptsPlasma membraneHyposmotic stimulationFluorescent membrane dye FM1-43Membrane dye FM1-43Trafficking of channelsClC-3 channelsCiliary epithelial cellsPhosphoinositide-3 kinase inhibitor wortmanninRate of exocytosisClC-3 Cl(-) channelsEpithelial cellsVesicular traffickingDye FM1-43Membrane dynamicsNonpigmented ciliary epithelial cellsInhibitor wortmanninClC-3 antisenseImmunofluorescence microscopyTraffickingClC-3Regulatory volume decreaseFM1-43Cl- channelsMembraneCell swelling
1999
Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein