2022
An incentive circuit for memory dynamics in the mushroom body of Drosophila melanogaster
Gkanias E, McCurdy LY, Nitabach MN, Webb B. An incentive circuit for memory dynamics in the mushroom body of Drosophila melanogaster. ELife 2022, 11: e75611. PMID: 35363138, PMCID: PMC8975552, DOI: 10.7554/elife.75611.Peer-Reviewed Original ResearchConceptsFlexible behavioral controlConditioning paradigmNeural mechanismsNegative reinforcementMemory acquisitionBehavioral controlMemory dynamicsExploration/exploitationDrosophila melanogasterPlasticity rulesMushroom bodiesComputational modellingAcquisitionMemorySpecific neuronsStimuliDifferent rolesParadigmDrosophilaMelanogasterInsectsShort termFindingsNeuronsDopaminergic
2021
A neuronal ensemble encoding adaptive choice during sensory conflict in Drosophila
Sareen PF, McCurdy LY, Nitabach MN. A neuronal ensemble encoding adaptive choice during sensory conflict in Drosophila. Nature Communications 2021, 12: 4131. PMID: 34226544, PMCID: PMC8257655, DOI: 10.1038/s41467-021-24423-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDecision MakingDrosophilaDrosophila melanogasterDrosophila ProteinsFeeding BehaviorFood PreferencesHungerNeuronsTasteTaste PerceptionDopaminergic mechanism underlying reward-encoding of punishment omission during reversal learning in Drosophila
McCurdy LY, Sareen P, Davoudian PA, Nitabach MN. Dopaminergic mechanism underlying reward-encoding of punishment omission during reversal learning in Drosophila. Nature Communications 2021, 12: 1115. PMID: 33602917, PMCID: PMC7893153, DOI: 10.1038/s41467-021-21388-w.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAvoidance LearningConditioning, ClassicalDopamineDopaminergic NeuronsDrosophila melanogasterMemoryPunishmentReversal LearningRewardSmellSynapsesConceptsDopaminergic neuronsCholinergic neuronsNeural circuit mechanismsCholinergic relayDopaminergic mechanismsSynaptic excitationSynaptic reconstructionSynaptic inputsVivo functional imagingCircuit mechanismsNeuronsAversive memoryFunctional imagingOdor responsesAversive outcomesReduced activationSuch activationCircuit motifsActivationOutcomesElectric shock punishmentSensory cuesUnexpected omissionShock punishmentBehavioral analysis
2017
Genetic and neuronal mechanisms governing the sex-specific interaction between sleep and sexual behaviors in Drosophila
Chen D, Sitaraman D, Chen N, Jin X, Han C, Chen J, Sun M, Baker BS, Nitabach MN, Pan Y. Genetic and neuronal mechanisms governing the sex-specific interaction between sleep and sexual behaviors in Drosophila. Nature Communications 2017, 8: 154. PMID: 28754889, PMCID: PMC5533705, DOI: 10.1038/s41467-017-00087-5.Peer-Reviewed Original ResearchA Peptidergic Circuit Links the Circadian Clock to Locomotor Activity
King AN, Barber AF, Smith AE, Dreyer AP, Sitaraman D, Nitabach MN, Cavanaugh DJ, Sehgal A. A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity. Current Biology 2017, 27: 1915-1927.e5. PMID: 28669757, PMCID: PMC5698909, DOI: 10.1016/j.cub.2017.05.089.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCircadian ClocksDrosophila melanogasterDrosophila ProteinsLocomotionMaleNeuropeptidesReceptors, Cell SurfaceConceptsLocomotor activitySubesophageal zonePeptidergic circuitsPars intercerebralisCorticotropin-releasing factorVentral nerve cordSite of actionReceptor 1Motor outputCircadian locomotor activityNerve cordNeuronsRelevant receptorsDrosophila brainHr rhythmsCircadian driveRhythmFeeding rhythmDiuretic hormone 44Minimal effectActivity rhythmsBehavioral rhythmsCircadian locomotionCircadian controlCord
2016
Presynaptic GABA Receptors Mediate Temporal Contrast Enhancement in Drosophila Olfactory Sensory Neurons and Modulate Odor-Driven Behavioral Kinetics
Raccuglia D, McCurdy LY, Demir M, Gorur-Shandilya S, Kunst M, Emonet T, Nitabach MN. Presynaptic GABA Receptors Mediate Temporal Contrast Enhancement in Drosophila Olfactory Sensory Neurons and Modulate Odor-Driven Behavioral Kinetics. ENeuro 2016, 3: eneuro.0080-16.2016. PMID: 27588305, PMCID: PMC4994068, DOI: 10.1523/eneuro.0080-16.2016.Peer-Reviewed Original ResearchConceptsOlfactory sensory neuronsPeripheral responsesGABA receptorsSensory neuronsContrast enhancementOSN axon terminalsInhibitory GABA receptorsPresynaptic GABAAxon terminalsDrosophila olfactory sensory neuronsPresynaptic terminalsNervous systemAuditory stimuliTemporal edgeOlfactory systemTime courseNeuronsInnate behavioral responsesReceptorsOptical electrophysiologyTemporal contrast enhancementBehavioral responsesLateral inhibitionResponseGABA
2015
Control of Sleep by Dopaminergic Inputs to the Drosophila Mushroom Body
Sitaraman D, Aso Y, Rubin GM, Nitabach MN. Control of Sleep by Dopaminergic Inputs to the Drosophila Mushroom Body. Frontiers In Neural Circuits 2015, 9: 73. PMID: 26617493, PMCID: PMC4637407, DOI: 10.3389/fncir.2015.00073.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedDopaminergic NeuronsDrosophila melanogasterImmunohistochemistryModels, AnimalMushroom BodiesNeural PathwaysSleepPropagation of Homeostatic Sleep Signals by Segregated Synaptic Microcircuits of the Drosophila Mushroom Body
Sitaraman D, Aso Y, Jin X, Chen N, Felix M, Rubin GM, Nitabach MN. Propagation of Homeostatic Sleep Signals by Segregated Synaptic Microcircuits of the Drosophila Mushroom Body. Current Biology 2015, 25: 2915-2927. PMID: 26455303, PMCID: PMC4654684, DOI: 10.1016/j.cub.2015.09.017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDrosophila melanogasterFemaleHomeostasisMaleMemoryMushroom BodiesNeuronsReproductionSleepSmellStructure-Activity RelationshipConceptsSynaptic microcircuitsDrosophila mushroom bodyKenyon cellsMushroom bodiesMB neuronsControl of sleepHomeostatic rebound sleepHomeostatic sleep regulationIncreases sleepRebound sleepSleep regulationMBONsSleep deprivationNeuron classesSleepSleep informationMemory centerSpecific functional connectionsFunctional connectionsNeuronsPhysiological approachDifferent populationsMicrocircuits
2014
Sensory determinants of behavioral dynamics in Drosophila thermotaxis
Klein M, Afonso B, Vonner AJ, Hernandez-Nunez L, Berck M, Tabone CJ, Kane EA, Pieribone VA, Nitabach MN, Cardona A, Zlatic M, Sprecher SG, Gershow M, Garrity PA, Samuel AD. Sensory determinants of behavioral dynamics in Drosophila thermotaxis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 112: e220-e229. PMID: 25550513, PMCID: PMC4299240, DOI: 10.1073/pnas.1416212112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBehavior, AnimalCalcium SignalingDrosophila melanogasterGangliaLarvaLocomotionOptogeneticsThermoreceptorsThermosensingCalcitonin Gene-Related Peptide Neurons Mediate Sleep-Specific Circadian Output in Drosophila
Kunst M, Hughes ME, Raccuglia D, Felix M, Li M, Barnett G, Duah J, Nitabach MN. Calcitonin Gene-Related Peptide Neurons Mediate Sleep-Specific Circadian Output in Drosophila. Current Biology 2014, 24: 2652-2664. PMID: 25455031, PMCID: PMC4255360, DOI: 10.1016/j.cub.2014.09.077.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcitonin Gene-Related PeptideCircadian RhythmDrosophila melanogasterDrosophila ProteinsInsect HormonesNeuronsSleepConceptsPigment-dispersing factorNeuropeptide calcitonin gene-related peptideCalcitonin gene-related peptideGene-related peptidePDF receptorClock neuronsCircadian clock neuronsDistinct neuronal pathwaysNeuropeptide pigment-dispersing factorDorsal clock neuronsAmount of sleepHomeostatic sleep driveNeurons actsCalcitonin geneNeuronal pathwaysTiming of sleepSleepMental healthSleep driveReceptorsNovel roleCircadian rhythmDH31NeuronsLocomotor rhythmMushroom body output neurons encode valence and guide memory-based action selection in Drosophila
Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guérin G, Plaçais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM. Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila. ELife 2014, 3: e04580. PMID: 25535794, PMCID: PMC4273436, DOI: 10.7554/elife.04580.Peer-Reviewed Original Research
2013
Genetically Targeted Optical Electrophysiology in Intact Neural Circuits
Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically Targeted Optical Electrophysiology in Intact Neural Circuits. Cell 2013, 154: 904-913. PMID: 23932121, PMCID: PMC3874294, DOI: 10.1016/j.cell.2013.07.027.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCircadian ClocksDrosophila melanogasterElectrophysiological PhenomenaGreen Fluorescent ProteinsNerve NetNeuronsOptogeneticsConceptsIntact neural circuitsNeural circuitsIntact brain tissueMembrane potentialNeuronal information processingNervous systemAction potentialsBrain tissueNeuronsStudy of intracellularElectrical activityKey cellular parametersMultiple neuronsElectrical eventsSubthreshold eventsNeurite branchesOptical electrophysiologyReliable recordingCellular parametersVoltage indicatorsFluorescent voltage indicatorsBrainA biogenic amine and a neuropeptide act identically: tyramine signals through calcium in Drosophila tubule stellate cells
Cabrero P, Richmond L, Nitabach M, Davies SA, Dow JA. A biogenic amine and a neuropeptide act identically: tyramine signals through calcium in Drosophila tubule stellate cells. Proceedings Of The Royal Society B 2013, 280: 20122943. PMID: 23446525, PMCID: PMC3619477, DOI: 10.1098/rspb.2012.2943.Peer-Reviewed Original ResearchMeSH KeywordsAequorinAnimalsApoproteinsCalcium SignalingChloridesDrosophila melanogasterDrosophila ProteinsGreen Fluorescent ProteinsInositol 1,4,5-Trisphosphate ReceptorsMalpighian TubulesModels, BiologicalNeuropeptidesPhospholipase C betaProtein EngineeringRecombinant ProteinsTyramineWater-Electrolyte BalanceConceptsTrisphosphate receptor geneCalcium signalsStellate cellsTranslational fusionInsect osmoregulationDistinct tissuesIntracellular calciumMode of actionPhospholipase CIntracellular calcium signalsReceptor geneIndependent mechanismsHalf-maximal activationTyramine-induced increaseUAS controlITPRTyramine actEndocrine controlRenal functionCellsNeuropeptides actPrincipal cellsKininsDrosophilaNorpA
2012
Autoreceptor Control of Peptide/Neurotransmitter Corelease from PDF Neurons Determines Allocation of Circadian Activity in Drosophila
Choi C, Cao G, Tanenhaus AK, McCarthy EV, Jung M, Schleyer W, Shang Y, Rosbash M, Yin JC, Nitabach MN. Autoreceptor Control of Peptide/Neurotransmitter Corelease from PDF Neurons Determines Allocation of Circadian Activity in Drosophila. Cell Reports 2012, 2: 332-344. PMID: 22938867, PMCID: PMC3432947, DOI: 10.1016/j.celrep.2012.06.021.Peer-Reviewed Original ResearchDeep sequencing the circadian and diurnal transcriptome of Drosophila brain
Hughes ME, Grant GR, Paquin C, Qian J, Nitabach MN. Deep sequencing the circadian and diurnal transcriptome of Drosophila brain. Genome Research 2012, 22: 1266-1281. PMID: 22472103, PMCID: PMC3396368, DOI: 10.1101/gr.128876.111.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsBase SequenceBrainCircadian ClocksCircadian RhythmDrosophila melanogasterExonsGene Expression ProfilingMolecular Sequence AnnotationPhotoperiodRNA EditingRNA IsoformsRNA Splice SitesRNA, UntranslatedSequence AlignmentSequence Analysis, RNATranscription, GeneticTranscriptomeConceptsKey circadian genesRNA editingNoncoding RNAsCircadian genesDrosophila brainGenome-wide mapsEukaryotic circadian clocksCircadian transcriptional rhythmsSnoRNA host genesTranslational feedback loopsSmall nucleolar RNAsNovel splicing eventsAlternative splice isoformsDark diurnal cyclesFrequency of RNAPrevious microarray studyDiurnal transcriptomeDrosophila melanogaster brainTranscriptional rhythmsCircadian transcriptomeNcRNA expressionRibosomal biogenesisModENCODE consortiumSplicing eventsAlternative splicing
2011
Insect circadian clock outputs
Helfrich-Förster C, Nitabach MN, Holmes TC. Insect circadian clock outputs. Essays In Biochemistry 2011, 49: 87-101. PMID: 21819386, DOI: 10.1042/bse0490087.Peer-Reviewed Original ResearchConceptsClock neuronsDaily rhythmsCircadian clock outputBrain clockCellular clocksDrosophila fliesCircadian timekeeping systemCircadian outputEnvironmental cuesClock outputMigratory locustInsectsTimekeeping systemCircadian rhythmicitySubstantial similarityClockImpressive varietyButterfliesLocal environmentFliesClock circuitEntire lifeLocustTimekeeperPhysiologySynchronized Bilateral Synaptic Inputs to Drosophila melanogaster Neuropeptidergic Rest/Arousal Neurons
McCarthy EV, Wu Y, deCarvalho T, Brandt C, Cao G, Nitabach MN. Synchronized Bilateral Synaptic Inputs to Drosophila melanogaster Neuropeptidergic Rest/Arousal Neurons. Journal Of Neuroscience 2011, 31: 8181-8193. PMID: 21632940, PMCID: PMC3125135, DOI: 10.1523/jneurosci.2017-10.2011.Peer-Reviewed Original ResearchConceptsSynaptic inputsWhole-cell patch-clamp recordingsNicotinic acetylcholine receptor antagonistDual whole-cell patch-clamp recordingsAcetylcholine receptor antagonistNicotinic ACh receptorsSynchronous synaptic inputPatch-clamp recordingsWake-promoting neuronsLarge ventrolateral neuronsArousal neuronsSodium blockersGABAergic inputsCholinergic inputReceptor antagonistSynaptic circuitryACh receptorsNeurotransmitter receptorsBilateral inputSynaptic connectionsΑ-bungarotoxinVentrolateral neuronsContralateral pairsFiring patternsNeurons