2024
Energy partitioning in the cell cortex
Chen S, Seara D, Michaud A, Kim S, Bement W, Murrell M. Energy partitioning in the cell cortex. Nature Physics 2024, 1-9. DOI: 10.1038/s41567-024-02626-6.Peer-Reviewed Original ResearchCell cortexEntropy production rateGAP expressionCortical actin filamentsRho GTPase pathwayGTPase pathwayMyosin IIActin filamentsDiversity patternsEnergy partitioningRhoOnsager reciprocityCell phenotypeProtein expressionThermodynamic equilibriumCellsSpiral travelling waveProduction rateTemporal dynamicsLiving systemsActinEnergyWavePhenotypeActivityComposite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model
Sakamoto R, Murrell M. Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model. Communications Biology 2024, 7: 840. PMID: 38987288, PMCID: PMC11236970, DOI: 10.1038/s42003-024-06528-4.Peer-Reviewed Original ResearchConceptsF-actin networkF-actinF-actin architectureMembrane shape changesCell shape changesActivity of myosinInduce membrane deformationActomyosin contractilityShape changesActin cortexActomyosin cortexGiant unilamellar vesiclesActinMembrane deformationUnilamellar vesiclesCell modelNo-slip boundariesForce generationActomyosinMyosinVesiclesForce transmissionActive tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex
Sakamoto R, Murrell M. Active tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex. Physical Review Research 2024, 6: 033024. DOI: 10.1103/physrevresearch.6.033024.Peer-Reviewed Original ResearchActomyosin cortexCell membraneActin cytoskeletonCortical flowMembrane blebbingCell divisionCell migrationCytoskeletonActomyosinBiological phenomenaMembrane bulgesBlebsCellsMembraneViscoelastic fluidMechanical responseElastic stressesStress yieldActinUbiquitous structuresApoptosisMechanical stressMembrane elasticityPhysical behaviorGrowth‐induced collective bending and kinetic trapping of cytoskeletal filaments
Banerjee D, Freedman S, Murrell M, Banerjee S. Growth‐induced collective bending and kinetic trapping of cytoskeletal filaments. Cytoskeleton 2024, 81: 409-419. PMID: 38775207, DOI: 10.1002/cm.21877.Peer-Reviewed Original ResearchActin networkFilamentous growthActin filamentsTurnover of actin filamentsActin filament growthKinetic trapsActin poolFilament polymerizationActin cortexCytoskeletal filamentsSubunit poolActinFilamentsSubunitConsequence of growthFilament mechanismNematic defectsCrowded environmentLong-livedGrowthPoolAbundanceBending patternCellsTurnoverConfinement induces internal flows in adherent cell aggregates
Yousafzai M, Amiri S, Sun Z, Pahlavan , Murrell M. Confinement induces internal flows in adherent cell aggregates. Journal Of The Royal Society Interface 2024, 21: 20240105. PMID: 38774959, PMCID: PMC11285874, DOI: 10.1098/rsif.2024.0105.Peer-Reviewed Original ResearchElastocapillary effects determine early matrix deformation by glioblastoma cell spheroids
Ang I, Yousafzai M, Yadav V, Mohler K, Rinehart J, Bouklas N, Murrell M. Elastocapillary effects determine early matrix deformation by glioblastoma cell spheroids. APL Bioengineering 2024, 8: 026109. PMID: 38706957, PMCID: PMC11069407, DOI: 10.1063/5.0191765.Peer-Reviewed Original ResearchF-actin architecture determines the conversion of chemical energy into mechanical work
Sakamoto R, Murrell M. F-actin architecture determines the conversion of chemical energy into mechanical work. Nature Communications 2024, 15: 3444. PMID: 38658549, PMCID: PMC11043346, DOI: 10.1038/s41467-024-47593-x.Peer-Reviewed Original ResearchConceptsF-actin architectureF-actinATP consumption rateF-actin bundlesIn vitro reconstitutionDynamic cellular processesHigher ATP consumptionActin cytoskeletonFilamentous actinMyosin motorsCellular processesATP hydrolysisPurified componentsAdenosine triphosphateForce generationConversion of chemical energyATP consumptionConsumption rateActinChemical energyMyosinNetwork contractionCytoskeletonEnergetic principlesHydrolysis
2023
Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change
Sun Z, Yadav V, Amiri S, Cao W, De La Cruz E, Murrell M. Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change. European Journal Of Cell Biology 2023, 103: 151379. PMID: 38168598, DOI: 10.1016/j.ejcb.2023.151379.Peer-Reviewed Original ResearchActin and Microtubules Position Stress Granules
Böddeker T, Rusch A, Leeners K, Murrell M, Dufresne E. Actin and Microtubules Position Stress Granules. PRX Life 2023, 1: 023010. DOI: 10.1103/prxlife.1.023010.Peer-Reviewed Original ResearchAuthor Correction: Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Author Correction: Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 470. PMID: 37117264, PMCID: PMC10147711, DOI: 10.1038/s42003-023-04818-x.Peer-Reviewed Original ResearchMembrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 325. PMID: 36973388, PMCID: PMC10043271, DOI: 10.1038/s42003-023-04684-7.Peer-Reviewed Original ResearchConceptsF-actin reorganizationMechanical stressCell cortexBiochemical regulationCell shapeActomyosin cortexPhysical behaviorCell polarizationMembrane tensionCell migrationEssential physical behaviorPore openingSpatial assemblyDrive changesMembraneRelative rolesStressActinRegulationDiverse behaviorsRoleAssemblyAuthor Correction: Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Author Correction: Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2023, 13: 4253. PMID: 36918662, PMCID: PMC10015071, DOI: 10.1038/s41598-023-31191-w.Peer-Reviewed Original ResearchF-actin architectures differentially constrain myosin thick filament motion
Muresan C, Sun Z, Yadav V, Tabatabai A, Lanier L, Kim J, Kim T, Murrell M. F-actin architectures differentially constrain myosin thick filament motion. Biophysical Journal 2023, 122: 294a. DOI: 10.1016/j.bpj.2022.11.1662.Peer-Reviewed Original Research
2022
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2022, 12: 22474. PMID: 36577792, PMCID: PMC9797577, DOI: 10.1038/s41598-022-26337-1.Peer-Reviewed Original ResearchConceptsCell-derived matricesCell stiffnessFamily verprolin-homologous protein 2Active Rac1Thrombospondin-2Homologous protein 2Less traction forceCytoskeleton organizationExtracellular matrix productionMajor cell populationF-actinCytoskeletal disorganizationRegulatory roleProtein 2Matrix productionCritical functionsECM productionArt microscopy techniquesNew functionsCell populationsSpindle-like shapeRac1Normal fibroblastsFibroblastsWound spaceF-actin architecture determines constraints on myosin thick filament motion
Muresan C, Sun Z, Yadav V, Tabatabai A, Lanier L, Kim J, Kim T, Murrell M. F-actin architecture determines constraints on myosin thick filament motion. Nature Communications 2022, 13: 7008. PMID: 36385016, PMCID: PMC9669029, DOI: 10.1038/s41467-022-34715-6.Peer-Reviewed Original ResearchInterplay between substrate rigidity and tissue fluidity regulates cell monolayer spreading
Staddon M, Murrell M, Banerjee S. Interplay between substrate rigidity and tissue fluidity regulates cell monolayer spreading. Soft Matter 2022, 18: 7877-7886. PMID: 36205535, PMCID: PMC9700261, DOI: 10.1039/d2sm00757f.Peer-Reviewed Original ResearchConceptsSubstrate rigidityCollective cell motionSubstrate stiffnessTissue fluidityStiff substratesCell-matrix adhesionTraction force generationCell-cell interactionsCell motionTissue morphogenesisSoft elastic matrixCell collectivesEmbryonic developmentCell crawlingCell spreadingCell monolayersSolid tissuesCell behaviorMechanical behaviorPredictive understandingMechanical propertiesCancer invasionCell aggregatesElastic matrixCell propertiesIn Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles.
Chen S, Sun Z, Murrell M. In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles. Journal Of Visualized Experiments 2022 PMID: 36094272, DOI: 10.3791/64026.Peer-Reviewed Original ResearchConceptsGiant unilamellar vesiclesCytoskeleton networkLipid dropletsCell‐mimicking systemUnilamellar vesiclesActin cytoskeletonVitro reconstitutionGenetic regulationActin networkBiochemical regulationSynthetic biologyCellular activitiesLive cellsMixture of proteinsActin polymersLipid componentsVesiclesRegulationReconstitutionCellsCytoskeletonCell deformationMachineryBiologyQuantitative insightsCell-Matrix Elastocapillary Interactions Drive Pressure-Based Wetting of Cell Aggregates
Yousafzai M, Yadav V, Amiri S, Staddon M, Errami Y, Jaspard G, Banerjee S, Murrell M. Cell-Matrix Elastocapillary Interactions Drive Pressure-Based Wetting of Cell Aggregates. Physical Review X 2022, 12: 031027. PMID: 38009085, PMCID: PMC10673637, DOI: 10.1103/physrevx.12.031027.Peer-Reviewed Original ResearchCapillary forcesCompliant substratesCapillary effectsRigid substrateData-driven modelingInternal pressureInterfacial tensionBulk elasticityCell surface tensionCell aggregatesSurface tensionActive dropletsMechanical stressCellular length scalesLength scalesComputational simulationsCollective cell motionDrive pressureWettingTissue shapeAdhesive substrateSubstrateElastocapillaryCell motionElasticityA molecular clock controls periodically driven cell migration in confined spaces
Lee SH, Hou JC, Hamidzadeh A, Yousafzai MS, Ajeti V, Chang H, Odde DJ, Murrell M, Levchenko A. A molecular clock controls periodically driven cell migration in confined spaces. Cell Systems 2022, 13: 514-529.e10. PMID: 35679858, DOI: 10.1016/j.cels.2022.05.005.Peer-Reviewed Original ResearchConceptsCell migrationSmall GTPase RhoAFaster cell migrationNegative feedback loopExchange factorMolecular clockGEF-H1RhoA guanineGTPase RhoAMicrotubule dynamicsMolecular mechanismsClock controlCell spreadExtracellular matrixIntracellular CaTissue reorganizationFeedback loopMigrationRhoAAbundanceGuanineBetter understandingCorrection: The structural, vibrational, and mechanical properties of jammed packings of deformable particles in three dimensions
Wang D, Treado JD, Boromand A, Norwick B, Murrell MP, Shattuck MD, O'Hern CS. Correction: The structural, vibrational, and mechanical properties of jammed packings of deformable particles in three dimensions. Soft Matter 2022, 18: 3815-3815. PMID: 35506750, PMCID: PMC9116153, DOI: 10.1039/d2sm90054h.Peer-Reviewed Original Research