2024
Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model
Sakamoto R, Murrell M. Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model. Communications Biology 2024, 7: 840. PMID: 38987288, PMCID: PMC11236970, DOI: 10.1038/s42003-024-06528-4.Peer-Reviewed Original ResearchConceptsF-actin networkF-actinF-actin architectureMembrane shape changesCell shape changesActivity of myosinInduce membrane deformationActomyosin contractilityShape changesActin cortexActomyosin cortexGiant unilamellar vesiclesActinMembrane deformationUnilamellar vesiclesCell modelNo-slip boundariesForce generationActomyosinMyosinVesiclesForce transmissionConfinement induces internal flows in adherent cell aggregates
Yousafzai M, Amiri S, Sun Z, Pahlavan , Murrell M. Confinement induces internal flows in adherent cell aggregates. Journal Of The Royal Society Interface 2024, 21: 20240105. PMID: 38774959, PMCID: PMC11285874, DOI: 10.1098/rsif.2024.0105.Peer-Reviewed Original ResearchF-actin architecture determines the conversion of chemical energy into mechanical work
Sakamoto R, Murrell M. F-actin architecture determines the conversion of chemical energy into mechanical work. Nature Communications 2024, 15: 3444. PMID: 38658549, PMCID: PMC11043346, DOI: 10.1038/s41467-024-47593-x.Peer-Reviewed Original ResearchConceptsF-actin architectureF-actinATP consumption rateF-actin bundlesIn vitro reconstitutionDynamic cellular processesHigher ATP consumptionActin cytoskeletonFilamentous actinMyosin motorsCellular processesATP hydrolysisPurified componentsAdenosine triphosphateForce generationConversion of chemical energyATP consumptionConsumption rateActinChemical energyMyosinNetwork contractionCytoskeletonEnergetic principlesHydrolysis
2023
Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 325. PMID: 36973388, PMCID: PMC10043271, DOI: 10.1038/s42003-023-04684-7.Peer-Reviewed Original ResearchConceptsF-actin reorganizationMechanical stressCell cortexBiochemical regulationCell shapeActomyosin cortexPhysical behaviorCell polarizationMembrane tensionCell migrationEssential physical behaviorPore openingSpatial assemblyDrive changesMembraneRelative rolesStressActinRegulationDiverse behaviorsRoleAssembly
2022
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2022, 12: 22474. PMID: 36577792, PMCID: PMC9797577, DOI: 10.1038/s41598-022-26337-1.Peer-Reviewed Original ResearchConceptsCell-derived matricesCell stiffnessFamily verprolin-homologous protein 2Active Rac1Thrombospondin-2Homologous protein 2Less traction forceCytoskeleton organizationExtracellular matrix productionMajor cell populationF-actinCytoskeletal disorganizationRegulatory roleProtein 2Matrix productionCritical functionsECM productionArt microscopy techniquesNew functionsCell populationsSpindle-like shapeRac1Normal fibroblastsFibroblastsWound spaceF-actin architecture determines constraints on myosin thick filament motion
Muresan C, Sun Z, Yadav V, Tabatabai A, Lanier L, Kim J, Kim T, Murrell M. F-actin architecture determines constraints on myosin thick filament motion. Nature Communications 2022, 13: 7008. PMID: 36385016, PMCID: PMC9669029, DOI: 10.1038/s41467-022-34715-6.Peer-Reviewed Original ResearchIn Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles.
Chen S, Sun Z, Murrell M. In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles. Journal Of Visualized Experiments 2022 PMID: 36094272, DOI: 10.3791/64026.Peer-Reviewed Original ResearchConceptsGiant unilamellar vesiclesCytoskeleton networkLipid dropletsCell‐mimicking systemUnilamellar vesiclesActin cytoskeletonVitro reconstitutionGenetic regulationActin networkBiochemical regulationSynthetic biologyCellular activitiesLive cellsMixture of proteinsActin polymersLipid componentsVesiclesRegulationReconstitutionCellsCytoskeletonCell deformationMachineryBiologyQuantitative insightsActive Regulation of Pressure and Volume Defines an Energetic Constraint on the Size of Cell Aggregates
Yousafzai M, Yadav V, Amiri S, Errami Y, Amiri S, Murrell M. Active Regulation of Pressure and Volume Defines an Energetic Constraint on the Size of Cell Aggregates. Physical Review Letters 2022, 128: 048103. PMID: 35148133, DOI: 10.1103/physrevlett.128.048103.Peer-Reviewed Original Research
2014
Actomyosin sliding is attenuated in contractile biomimetic cortices
Murrell M, Gardel M. Actomyosin sliding is attenuated in contractile biomimetic cortices. Molecular Biology Of The Cell 2014, 25: 1845-1853. PMID: 24760970, PMCID: PMC4055264, DOI: 10.1091/mbc.e13-08-0450.Peer-Reviewed Original ResearchChapter Fifteen Reconstitution of Contractile Actomyosin Arrays
Murrell M, Thoresen T, Gardel M. Chapter Fifteen Reconstitution of Contractile Actomyosin Arrays. Methods In Enzymology 2014, 540: 265-282. PMID: 24630112, PMCID: PMC4459579, DOI: 10.1016/b978-0-12-397924-7.00015-7.Peer-Reviewed Original Research
2012
F-actin buckling coordinates contractility and severing in a biomimetic actomyosin cortex
Murrell M, Gardel M. F-actin buckling coordinates contractility and severing in a biomimetic actomyosin cortex. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 20820-20825. PMID: 23213249, PMCID: PMC3529094, DOI: 10.1073/pnas.1214753109.Peer-Reviewed Original Research
2011
Spreading Dynamics of Biomimetic Actin Cortices
Murrell M, Pontani L, Guevorkian K, Cuvelier D, Nassoy P, Sykes C. Spreading Dynamics of Biomimetic Actin Cortices. Biophysical Journal 2011, 100: 1400-1409. PMID: 21402021, PMCID: PMC3059579, DOI: 10.1016/j.bpj.2011.01.038.Peer-Reviewed Original Research