2022
Plasmodium falciparum coinfection is associated with improved IgE and IgG3 response against hookworm antigens
Sakyi SA, Wilson MD, Adu B, Opoku S, Brewoo A, Larbi A, Baafour EK, Tchum SK, Saahene RO, Aniagyei W, Sewor C, Courtin D, Cappello M, Gyan B, Amoani B. Plasmodium falciparum coinfection is associated with improved IgE and IgG3 response against hookworm antigens. Health Science Reports 2022, 5: e672. PMID: 35734341, PMCID: PMC9195015, DOI: 10.1002/hsr2.672.Peer-Reviewed Original ResearchL3 stage larvaeAlbendazole treatmentHookworm infectionImmune responseNonendemic controlsPlasmodium falciparum coinfectionsIron deficiency anemiaHookworm treatmentVaccine effectivenessIgG3 responsesIgG responsesAntibody profileSingle doseDeficiency anemiaIgG3 antibodiesBlood samplesIgEProtein malnutritionControl groupVaccine developmentHigh levelsSerum samplesIgG3Stage larvaeInfection
2013
Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection
Wiśniewski M, Jaros S, Bąska P, Cappello M, Wędrychowicz H. Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection. Acta Parasitologica 2013, 58: 376-383. PMID: 23990436, DOI: 10.2478/s11686-013-0151-9.Peer-Reviewed Original ResearchConceptsChallenge infectionAnthelminthic drug resistanceEffective human vaccineWorm burden reductionsGroups of hamstersIron deficiency anemiaCDNA vaccineTriple immunizationInfected childrenDeficiency anemiaDNA vaccineHookworm infectionDNA vaccinationHuman vaccinesAnimal modelsNumerous antigensVaccine developmentGrowth retardationIntestinal nematodesDrug resistanceVaccineBurden reductionPartial protectionInfectionHemoglobin digestion
2006
Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo
Held MR, Bungiro RD, Harrison LM, Hamza I, Cappello M. Dietary Iron Content Mediates Hookworm Pathogenesis In Vivo. Infection And Immunity 2006, 74: 289-295. PMID: 16368983, PMCID: PMC1346670, DOI: 10.1128/iai.74.1.289-295.2006.Peer-Reviewed Original ResearchConceptsDay 20 postinfectionGrowth delayIron-restricted dietHigh-iron dietIntestinal worm burdenDietary iron restrictionIron deficiency anemiaBlood hemoglobin levelsDietary iron contentGreater weight lossSignificant growth delayHemoglobin levelsDeficiency anemiaHookworm infectionDiet groupHamster modelSevere diseaseStandard dietUninfected controlsHemoglobin concentrationUninfected animalsWorm burdenDay 10Infected animalsHookworm disease
2004
Ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors Xa and VIIa
Mieszczanek J, Harrison LM, Cappello M. Ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors Xa and VIIa. Molecular And Biochemical Parasitology 2004, 137: 151-159. PMID: 15279961, DOI: 10.1016/j.molbiopara.2004.05.011.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAmino acid sequence similarityFactor XaGastrointestinal blood lossIron deficiency anemiaSite amino acid residuesAmino acid residuesSite amino acidsMechanism of actionBlood lossHookworm infectionSequence similarityDeficiency anemiaLeading causeFactor VIIa/tissue factor complexFactor complexCoagulation inhibitorsPeptide-1Ancylostoma caninum anticoagulant peptide 5Coagulation responseAcid residuesNon-active siteA. ceylanicumAnticoagulantsTissue factor complexMolecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay
Chu D, Bungiro RD, Ibanez M, Harrison LM, Campodonico E, Jones BF, Mieszczanek J, Kuzmic P, Cappello M. Molecular Characterization of Ancylostoma ceylanicum Kunitz-Type Serine Protease Inhibitor: Evidence for a Role in Hookworm-Associated Growth Delay. Infection And Immunity 2004, 72: 2214-2221. PMID: 15039345, PMCID: PMC375216, DOI: 10.1128/iai.72.4.2214-2221.2004.Peer-Reviewed Original ResearchConceptsGrowth delayAdult hookwormsIron deficiency anemiaPolyclonal immunoglobulin GDeficiency anemiaHookworm infectionReverse transcription-PCRKunitz-type serine protease inhibitorImmunohistochemistry studiesBroad-spectrum inhibitorPartial protectionMajor causeImmunoglobulin GSerine protease inhibitorIntestinal proteasesThird-stage larvaeTranscription-PCRProtease inhibitorsAnemiaHookwormVivo roleMalnutritionInhibitorsInfected hostPancreatic elastase
2003
Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum
Del Valle A, Jones BF, Harrison LM, Chadderdon RC, Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Molecular And Biochemical Parasitology 2003, 129: 167-177. PMID: 12850261, DOI: 10.1016/s0166-6851(03)00121-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsAntibodies, HelminthChromatography, High Pressure LiquidCloning, MolecularCollagenDNA, ComplementaryFibrinogenHelminth ProteinsImmunoglobulin GImmunohistochemistryIntegrin alpha2beta1Molecular Sequence DataPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexRecombinant ProteinsSpectrometry, Mass, Electrospray IonizationConceptsIron deficiency anemiaCell surface integrin receptorsIdentical N-terminalAmino acid sequence homologyAmino acid sequenceSingle gene productSurface integrin receptorsDeficiency anemiaMammalian hemostasisCorresponding native proteinsCDNA correspondingSignificant homologyMolecular cloningSimilar molecular massSecreted proteinsGene productsSequence homologyAcid sequenceInhibitor proteinHPI proteinVaccine-based strategiesNative proteinN-terminalAdult Ancylostoma caninumAdult stage
2001
Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *
Harrison LM, Nerlinger A, Bungiro RD, Córdova JL, Kuzmič P, Cappello M. Molecular Characterization of Ancylostoma Inhibitors of Coagulation Factor Xa HOOKWORM ANTICOAGULANT ACTIVITY IN VITRO PREDICTS PARASITE BLOODFEEDING IN VIVO *. Journal Of Biological Chemistry 2001, 277: 6223-6229. PMID: 11741914, DOI: 10.1074/jbc.m109908200.Peer-Reviewed Original ResearchConceptsCoagulation factor XaAdult A. ceylanicumHookworm speciesHuman vaccine developmentIron deficiency anemiaAnticoagulant activityFactor Xa inhibitory activityFactor Xa activityDog hookworm Ancylostoma caninumFactor XaXa inhibitory activityMechanism of actionFirst anticoagulantGastrointestinal hemorrhageDeficiency anemiaReverse transcription-PCRSpecies of hookwormXa activityPeptide-1Vaccine developmentHookworm Ancylostoma caninumA. ceylanicumAncylostoma ceylanicumHookwormAncylostoma caninumAncylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic
Harrison L, Córdova J, Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Molecular And Biochemical Parasitology 2001, 115: 101-107. PMID: 11377744, DOI: 10.1016/s0166-6851(01)00276-6.Peer-Reviewed Original ResearchConceptsPolyclonal IgGAnticoagulant activityFuture immunization strategiesGastrointestinal blood lossIron deficiency anemiaPartial thromboplastin timeFactor Xa activityHuman coagulation factor XaBlood lossDeficiency anemiaHookworm infectionThromboplastin timeXa activityAmphidial glandsAdult Ancylostoma caninum hookwormsCoagulation factor XaAdult hookwormsImmunization strategiesA. ceylanicumIgGMajor causeHookwormSerine protease inhibitorAdult wormsViable target
1999
The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro
Chadderdon R, Cappello M. The Hookworm Platelet Inhibitor: Functional Blockade of Integrins GPIIb/IIIa (αIIbβ3) and GPIa/IIa (α2β1) Inhibits Platelet Aggregation and Adhesion In Vitro. The Journal Of Infectious Diseases 1999, 179: 1235-1241. PMID: 10191228, DOI: 10.1086/314724.Peer-Reviewed Original ResearchConceptsIntegrin GPIIb/IIIaPlatelet inhibitorsSoluble protein extractsGPIIb/IIIaPlatelet aggregationMolecular massProtein extractsIron deficiency anemiaGPIa/IIaInhibitory monoclonal antibodiesVariety of agonistsAnti-platelet activityAdult Ancylostoma caninum hookwormsInhibits platelet aggregationPlatelet receptorsDeficiency anemiaFunctional blockadeSpecific blockadeBiologic roleSize exclusion chromatographyIntestinal nematodesSecretory productsMonoclonal antibodiesInhibitorsAdult worms
1995
Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa.
Cappello M, Vlasuk G, Bergum P, Huang S, Hotez P. Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6152-6156. PMID: 7597095, PMCID: PMC41660, DOI: 10.1073/pnas.92.13.6152.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAnimalsArthropod ProteinsBlood CoagulationChromatography, GelChromatography, High Pressure LiquidChromatography, Ion ExchangeFactor Xa InhibitorsHelminth ProteinsHirudinsHumansIntercellular Signaling Peptides and ProteinsKineticsMass SpectrometryMolecular Sequence DataPeptide FragmentsPeptidesProthrombin TimeRecombinant ProteinsSerine Proteinase InhibitorsTicksConceptsBlood lossFactor XaIntestinal blood lossGastrointestinal blood lossHuman hookworm infectionIron deficiency anemiaSoil-transmitted helminthsHuman coagulation factor XaFree factor XaAnticoagulant peptideSpecific inhibitorHookworm infectionDeficiency anemiaProthrombin timeIntestinal mucosaCoagulation factor XaMajor causeRecombinant hirudinChromogenic assayMajor anticoagulantAdult wormsAnticoagulantsProtease inhibitorsHuman coagulationInhibitors