2020
Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane
Wu J, Rowart P, Jouret F, Gassaway BM, Rajendran V, Rinehart J, Caplan MJ. Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane. American Journal Of Physiology - Cell Physiology 2020, 318: c486-c501. PMID: 31913699, PMCID: PMC7099514, DOI: 10.1152/ajpcell.00422.2019.Peer-Reviewed Original ResearchConceptsJunction assemblyPlasma membranePhospho-defective mutantsEpithelial junction assemblyMDCK renal epithelial cellsProtein kinase activationJunction-associated proteinsRenal epithelial cellsActive GTPEpithelial polarizationTight junction componentsZO-1 localizationAMPK activationKinase activationKey regulatorAfadinAMPKImportant regulatorJunction componentsProtein zonula occludens-1Par3Epithelial cellsTight junction protein zonula occludens-1RegulatorAssembly
2010
Lymphocytes Accelerate Epithelial Tight Junction Assembly: Role of AMP-Activated Protein Kinase (AMPK)
Tang XX, Chen H, Yu S, Zhang L, Caplan MJ, Chan HC. Lymphocytes Accelerate Epithelial Tight Junction Assembly: Role of AMP-Activated Protein Kinase (AMPK). PLOS ONE 2010, 5: e12343. PMID: 20808811, PMCID: PMC2925955, DOI: 10.1371/journal.pone.0012343.Peer-Reviewed Original ResearchConceptsTJ assemblyActivation of AMPKProtein kinaseEpithelial cellsCalcium switch experimentsEpithelial cell polaritySuppression of AMPKTight junction assemblyRole of AMPMadin-Darby canine kidney cellsCellular ATP levelsCanine kidney cellsTight junctionsCell polarityApicolateral borderJunction assemblyModel cell lineAMPK activationProper formationAdjacent epithelial cellsTJ formationChemical inhibitorsCalu-3 human airway epithelial cellsHuman airway epithelial cellsMDCK cells
2006
AMP-activated protein kinase regulates the assembly of epithelial tight junctions
Zhang L, Li J, Young LH, Caplan MJ. AMP-activated protein kinase regulates the assembly of epithelial tight junctions. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 17272-17277. PMID: 17088526, PMCID: PMC1859922, DOI: 10.1073/pnas.0608531103.Peer-Reviewed Original ResearchConceptsTight junction assemblyJunction assemblyProtein kinaseLKB1-dependent phosphorylationCell polarization processCellular energy statusActivation of AMPKTight junctionsEukaryotic cellsTight junction structureAMPKMDCK cellsEpithelial tight junctionsEnergy statusKinaseEpithelial cellsAbsence of Ca2AssemblyTransepithelial electrical resistanceParacellular fluxZonula occludens-1CellsRecent studiesOccludens-1LKB1