2023
Loops and the activity of loop extrusion factors constrain chromatin dynamics
Bailey M, Surovtsev I, Williams J, Yan H, Yuan T, Li K, Duseau K, Mochrie S, King M. Loops and the activity of loop extrusion factors constrain chromatin dynamics. Molecular Biology Of The Cell 2023, 34: ar78. PMID: 37126401, PMCID: PMC10398873, DOI: 10.1091/mbc.e23-04-0119.Peer-Reviewed Original ResearchConceptsChromatin dynamicsChromatin mobilityChromatin conformation capture experimentsINO80 chromatin remodelerSystematic genetic perturbationsDynamics of chromatinSWI/SNFChromatin fluctuationsCondensin complexRSC complexChromatin remodelersFission yeastChromosome structureChromatin polymerExtrusion factorsChromatin motionGenetic perturbationsThree-dimensional structureDNA structureChromatinCohesinPolymer simulationsIntroduction of loopsKey roleActive process
2018
Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks
Leland BA, Chen AC, Zhao AY, Wharton RC, King MC. Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks. ELife 2018, 7: e33402. PMID: 29697047, PMCID: PMC5945276, DOI: 10.7554/elife.33402.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksDouble-strand breaksLong-range resectionPARPi resistanceBRCA1-deficient cellsHomology-directed repairExonuclease Exo1RecQ helicaseNucleolytic processingMicroscopy assaysREV7Cancer cellsMachineryPreclinical modelsResectionPolymerase inhibitorsPathwayCellsRecQOrthologuesHelicaseExo1AssaysDNA2Breaks
2014
The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body
Wälde S, King MC. The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body. Journal Of Cell Science 2014, 127: 3625-3640. PMID: 24963130, PMCID: PMC4132395, DOI: 10.1242/jcs.154997.Peer-Reviewed Original ResearchConceptsSpindle pole bodyMitotic entryPole bodySUN domain protein Sad1Nuclear envelopeMitotic spindle pole bodiesPolo kinase Plo1SUN-KASH complexesYeast centrosome equivalentBipolar spindle formationCentrosome equivalentMitotic remodelingSchizosaccharomyces pombeSpindle formationSpindle polesMonopolar spindlesMitotic catastrophePlo1Cut12PCP1KMS2Sad1PombeBiogenesisRemodeling
2008
A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules
King MC, Drivas TG, Blobel G. A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules. Cell 2008, 134: 427-438. PMID: 18692466, PMCID: PMC2617791, DOI: 10.1016/j.cell.2008.06.022.Peer-Reviewed Original ResearchConceptsSUN-KASH complexesNuclear membrane proteinsMembrane proteinsIntegral inner nuclear membrane proteinsSUN domain protein Sad1Nuclear envelopeSpindle pole body componentInner nuclear membrane proteinFission yeast S. pombeOuter nuclear membrane proteinsCytoplasmic microtubulesYeast S. pombeMicrotubule-dependent forcesNuclear envelope membranesCentromeric DNAS. pombeCentromeric heterochromatinHeterochromatic regionsEnvelope membraneIMA1MicrotubulesSad1HeterochromatinProteinNuclear heterochromatin