The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis
Balart J, Pueyo G, de Llobet L, Baro M, Sole X, Marin S, Casanovas O, Mesia R, Capella G. The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis. Radiation Oncology 2011, 6: 6. PMID: 21235815, PMCID: PMC3025872, DOI: 10.1186/1748-717x-6-6.Peer-Reviewed Original ResearchConceptsRadiation-induced DNA repairDNA repairCaspase inhibitorsDNA double-strand break repairChemical caspase inhibitorsSpontaneous DNA breakageDNA fragmentationDouble-strand break repairInterference of apoptosisSpecific caspase inhibitorsPulsed-field gel electrophoresisSpontaneous DNA fragmentationGel electrophoresisCell fateBreak repairDNA fragmentsApoptosis inhibitorH2AX activationAgarose plugsDNA breakageApoptosis inhibitionFunctional assaysCaspase-3ApoptosisCell cultures