2003
Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus
Habib-Bein NF, Beckwith WH, Mayo D, Landry ML. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus. Journal Of Clinical Microbiology 2003, 41: 3597-3601. PMID: 12904361, PMCID: PMC179819, DOI: 10.1128/jcm.41.8.3597-3601.2003.Peer-Reviewed Original ResearchConceptsVirus-positive specimensDirect immunofluorescence antibody stainingPublic health laboratoriesHealth laboratoriesRT-PCRMedical CenterRapid diagnosisYale-New Haven HospitalReal-time RT-PCRResults of cultureRhesus monkey kidney cell culturesImmunofluorescence antibody stainingReal-time reverse transcriptionInfluenza A virusesMonkey kidney cell culturesConventional RT-PCR analysisReal-time RT-PCR systemRespiratory specimensInfluenza APatient managementDirect immunofluorescenceCell culturesKidney cell culturesRapid turnaround timeA virusRapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification
Landry ML, Garner R, Ferguson D. Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification. Journal Of Clinical Microbiology 2003, 41: 346-350. PMID: 12517871, PMCID: PMC149595, DOI: 10.1128/jcm.41.1.346-350.2003.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationPrimary rhesus monkey kidneyRNA detectionNucliSens Basic KitRhesus monkey kidneyGreen monkey kidney cellsBuffalo green monkey kidney cellsMonkey kidney cellsVirus isolationClinical specimensEnterovirus detectionMonkey kidneyBasic KitMRC-5Kidney cellsCell culturesEV isolationRhabdomyosarcomaKidney
2000
SimulFluor Respiratory Screen for Rapid Detection of Multiple Respiratory Viruses in Clinical Specimens by Immunofluorescence Staining
Landry M, Ferguson D. SimulFluor Respiratory Screen for Rapid Detection of Multiple Respiratory Viruses in Clinical Specimens by Immunofluorescence Staining. Journal Of Clinical Microbiology 2000, 38: 708-711. PMID: 10655371, PMCID: PMC86182, DOI: 10.1128/jcm.38.2.708-711.2000.Peer-Reviewed Original ResearchConceptsCommon respiratory virusesRespiratory virusesMultiple respiratory virusesVirus isolatesRespiratory samplesPatient managementDirect immunofluorescenceInfection controlClinical specimensCytospin preparationsVirusScreening reagentImmunofluorescenceCell culturesSample collectionGreat benefitIsolates
1991
Advantages of multiple cell culture systems for detection of mixed-virus infections
Fong C, Landry M. Advantages of multiple cell culture systems for detection of mixed-virus infections. Journal Of Virological Methods 1991, 33: 283-289. PMID: 1783674, DOI: 10.1016/0166-0934(91)90027-w.Peer-Reviewed Original ResearchConceptsMRC-5 cellsHSV-1MRC-5CMV-positive casesA549 cellsHSV-1 isolatesCell culturesRhesus monkey kidney cell culturesMonkey kidney cell culturesHuman continuous cell lineImmunosuppressed patientsCytomegalovirus antigenFibroblast cell strainsCentrifugation cultureKidney cell culturesViral agentsSimultaneous infectionContinuous cell linesCulture systemShell vialsMore virusesAdenovirus isolatesEmbryo cell culturesCell culture systemMixed virus infections
1987
Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining
Zhao LS, Landry ML, Balkovic ES, Hsiung GD. Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining. Journal Of Clinical Microbiology 1987, 25: 1401-1405. PMID: 3040800, PMCID: PMC269234, DOI: 10.1128/jcm.25.8.1401-1405.1987.Peer-Reviewed Original ResearchConceptsHerpes simplex virusSimplex virusCytopathic effectHerpes simplex virus (HSV) isolationMink lungVero cellsOverall isolation rateViral antigen detectionViral infectivity titerCell culturesApplication of immunoperoxidaseCommercial kitsMRC-5 cellsRapidity of detectionRabbit kidney cellsCulture sensitivityVirus isolationClinical specimensInfectious virusInfectivity titersHigh titersAntigen detectionIsolation rateImmunoperoxidaseRabbit kidney
1985
Nucleic Acid Hybridization in the Diagnosis of Viral Infections
Landry M, Fong C. Nucleic Acid Hybridization in the Diagnosis of Viral Infections. Clinics In Laboratory Medicine 1985, 5: 513-529. PMID: 3899479, DOI: 10.1016/s0272-2712(18)30857-6.Peer-Reviewed Original ResearchConceptsNucleic acid hybridizationRecombinant DNA technologyAcid hybridizationHigh specific activityM13 bacteriophageDNA technologyEpstein-Barr virusStandard isolationStable probesRoutine diagnostic laboratorySample DNABiotinylated probesSensitive assaySpecific activityHepatitis B virusVaricella-zoster virusSpecific diagnostic testsReaction timeClinical virologyCell culturesHybridization techniqueDiagnostic laboratoriesProbeSynthesisHuman papillomavirus