Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
Bandyopadhyay U, Fenton WA, Horwich AL, Nagy M. Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection. Journal Of Visualized Experiments 2014, 51168. PMID: 24457537, PMCID: PMC4089401, DOI: 10.3791/51168.Peer-Reviewed Original ResearchConceptsLaser capture microdissectionMotor neuronsSpinal cordMotor neuron cell bodiesCapture microdissectionMouse spinal cordNeuron cell bodiesTotal cell populationCell typesPharmacologic treatmentStained neuronsCell bodiesNeurodegenerative diseasesNeuronsExogenous RNasesQRT-PCRSame cell typeCell populationsCordRNA damageDiseaseTotal RNASuch preparationsMicrodissectionTranscriptional differences