2020
Differential regulation of macrophage activation by the MIF cytokine superfamily members MIF and MIF‐2 in adipose tissue during endotoxemia
Kim B, Tilstam PV, Arnke K, Leng L, Ruhl T, Piecychna M, Schulte W, Sauler M, Frueh FS, Storti G, Lindenblatt N, Giovanoli P, Pallua N, Bernhagen J, Bucala R. Differential regulation of macrophage activation by the MIF cytokine superfamily members MIF and MIF‐2 in adipose tissue during endotoxemia. The FASEB Journal 2020, 34: 4219-4233. PMID: 31961019, PMCID: PMC7060131, DOI: 10.1096/fj.201901511r.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorWhite adipose tissueAdipose tissue macrophagesAdipose tissueElevated macrophage migration inhibitory factorInflammatory cytokine macrophage migration inhibitory factorVisceral white adipose tissueCytokine macrophage migration inhibitory factorPro-inflammatory macrophage polarizationMIF family proteinsPro-inflammatory phenotypeAnti-inflammatory phenotypeMIF-2Migration inhibitory factorWild-type miceHuman adipose tissueMurine endotoxemia modelMIF cytokineMIF deficiencySystemic inflammationDifferential regulationHealthy patientsEndotoxemia modelPeritoneal fluidLeading cause
2018
Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation
Pellowe AS, Sauler M, Hou Y, Merola J, Liu R, Calderon B, Lauridsen HM, Harris MR, Leng L, Zhang Y, Tilstam PV, Pober JS, Bucala R, Lee PJ, Gonzalez AL. Endothelial cell‐secreted MIF reduces pericyte contractility and enhances neutrophil extravasation. The FASEB Journal 2018, 33: 2171-2186. PMID: 30252532, PMCID: PMC6338650, DOI: 10.1096/fj.201800480r.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBronchoalveolar Lavage FluidCells, CulturedEndothelium, VascularEnzyme-Linked Immunosorbent AssayHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMiceMice, KnockoutNeutrophilsPericytesConceptsMacrophage migration inhibitory factorNeutrophil transmigrationNeutrophil extravasationPericyte contractilitySelective deletionMIF knockout miceAcute lung injuryMigration inhibitory factorHuman neutrophil transmigrationMyosin light chain phosphorylationLung injuryNeutrophil infiltrationBronchoalveolar lavageInflammatory disordersKnockout miceInhibitory factorMicro-ELISAContractilityLight chain phosphorylationPhosphorylated myosin light chainEndothelial cellsBarrier functionExtravasationPericytesParacrine signals