2024
Understanding Pathophysiology of Acute Interstitial Nephritis through Spatial Characterization of Cell-Cell Interactions Using Imaging Mass Cytometry in a Human Biopsy Cohort
Baker M, Kakade V, Budiman T, Weiss M, Shelar A, Moledina D, Cantley L. Understanding Pathophysiology of Acute Interstitial Nephritis through Spatial Characterization of Cell-Cell Interactions Using Imaging Mass Cytometry in a Human Biopsy Cohort. Journal Of The American Society Of Nephrology 2024, 35: 10.1681/asn.2024n6j4nxq1. DOI: 10.1681/asn.2024n6j4nxq1.Peer-Reviewed Original Research
2021
Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues
Kakade VR, Weiss M, Cantley LG. Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues. Frontiers In Physiology 2021, 12: 817181. PMID: 35002783, PMCID: PMC8727440, DOI: 10.3389/fphys.2021.817181.Peer-Reviewed Original ResearchSingle-cell sequencingComplex cellular microenvironmentHuman disease pathogenesisKidney cell typesCell identityHuman kidney samplesMultiplexed imaging techniquesMass cytometryCell sequencingCellular microenvironmentCell typesNovel insightsImaging Mass CytometryHuman tissuesDisease pathogenesisPathophysiological processesCellular dataAntibody validationProteomicsCytometryHuman kidneyIMC analysisSequencingKidney samplesPhenotyping
2019
Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry
Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight 2019, 4: e129477. PMID: 31217358, PMCID: PMC6629112, DOI: 10.1172/jci.insight.129477.Peer-Reviewed Original ResearchConceptsCell typesIndividual cell typesCritical baseline dataRenal cell typesMass cytometryQuantitative atlasNormal human samplesHuman kidneyRelative abundanceDevelopment of therapiesHuman kidney diseaseKidney diseaseMetal-conjugated antibodiesQuantitative interrogationScarce samplesMachine-learning pipelineDiscovery purposesFuture quantitative analysisNovel abnormalityNormal human kidneySingle tissue sectionHuman samplesRenal biopsyImmune cellsCells