2023
Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis
Egozi A, Olaloye O, Werner L, Silva T, McCourt B, Pierce R, An X, Wang F, Chen K, Pober J, Shouval D, Itzkovitz S, Konnikova L. Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis. PLOS Biology 2023, 21: e3002124. PMID: 37205711, PMCID: PMC10234541, DOI: 10.1371/journal.pbio.3002124.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingSingle-cell atlasEpithelial cellsCell identityRNA sequencingBulk transcriptomicsCellular dysregulationAberrant interactionsNeonatal small intestinePotential targetCellular changesBiomarker discoveryGastrointestinal complicationsPremature infantsProinflammatory macrophagesProinflammatory genesClonal expansionT cellsEndothelial cellsImmune interactionsIntestinal tissueCellsSmall intestineComprehensive viewNECSingle cell analysis via mass cytometry of spontaneous intestinal perforation reveals alterations in small intestinal innate and adaptive mucosal immunity
Olaloye O, Eke C, Jolteus A, Konnikova L. Single cell analysis via mass cytometry of spontaneous intestinal perforation reveals alterations in small intestinal innate and adaptive mucosal immunity. Frontiers In Immunology 2023, 14: 995558. PMID: 36825028, PMCID: PMC9941693, DOI: 10.3389/fimmu.2023.995558.Peer-Reviewed Original ResearchConceptsSpontaneous intestinal perforationMucosal immune dysfunctionSevere gastrointestinal complicationsSmall intestinal mucosaMass cytometry timeGastrointestinal complicationsIntestinal perforationEnteral feedsImmune dysfunctionPremature infantsWeeks' gestationTerminal ileumCytometry timeIntestinal mucosaDisease pathogenesisLocalized perforationFirst weekPatientsSurgeryGestationPerforationPrematurityComplicationsDysfunctionMucosa
2021
CD16+CD163+ monocytes traffic to sites of inflammation during necrotizing enterocolitis in premature infants
Olaloye OO, Liu P, Toothaker JM, McCourt BT, McCourt CC, Xiao J, Prochaska E, Shaffer S, Werner L, Faculty U, Faculty U, Gringauz J, Good M, Goldsmith JD, An X, Wang F, Snapper SB, Shouval D, Chen K, Tseng G, Konnikova L. CD16+CD163+ monocytes traffic to sites of inflammation during necrotizing enterocolitis in premature infants. Journal Of Experimental Medicine 2021, 218: e20200344. PMID: 34269788, PMCID: PMC8289692, DOI: 10.1084/jem.20200344.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticBlood VesselsCase-Control StudiesChemotaxisEnterocolitis, NecrotizingGastric MucosaGPI-Linked ProteinsHumansInfantInfant, NewbornIntestine, SmallMonocytesNeutropeniaNeutrophilsPhagocytosisReactive Oxygen SpeciesReceptors, Cell SurfaceReceptors, IgGSequence Analysis, RNASingle-Cell AnalysisConceptsSurgical NECCirculation of infantsDistinct neutrophil phenotypesSevere gastrointestinal complicationsAreas of inflammationSites of inflammationMonocyte trafficGastrointestinal complicationsPremature infantsNeutrophil phenotypeSevere inflammationInflammatory genesPotential biomarkersSingle-cell RNA sequencingOxygen species generationInflammationNovel subtypeBlood vesselsMass cytometryNECEnterocolitisMucosaInfantsMϕsSpecies generation