Featured Publications
Comparison of Female Ovarian Reserve Before vs After COVID-19 Vaccination
Yang L, Neal S, Lee T, Chou A, Schutt A, Gibbons W. Comparison of Female Ovarian Reserve Before vs After COVID-19 Vaccination. JAMA Network Open 2023, 6: e2318804. PMID: 37326996, PMCID: PMC10276301, DOI: 10.1001/jamanetworkopen.2023.18804.Peer-Reviewed Original ResearchMachine learning in time-lapse imaging to differentiate embryos from young vs old mice
Yang L, Leynes C, Pawelka A, Lorenzo I, Chou A, Lee B, Heaney J. Machine learning in time-lapse imaging to differentiate embryos from young vs old mice. Biology Of Reproduction 2024, 110: 1115-1124. PMID: 38685607, PMCID: PMC11180621, DOI: 10.1093/biolre/ioae056.Peer-Reviewed Original ResearchMaternal agePreimplantation genetic testingMorphokinetic parameters of embryosEarly embryo developmentMaternal miceNo significant differenceEmbryo transferTime-lapse microscopyGenetic testingAge-related phenotypesHuman embryosYoung donorsSignificant differenceNon-invasive approachEmbryo developmentCleavage stagesYounger counterpartsMiceNon-invasive technologyEmbryosAged embryosPhenotypeAgeTime-lapse imagingMorphokineticsDevelopment of a dynamic machine learning algorithm to predict clinical pregnancy and live birth rate with embryo morphokinetics
Yang L, Peavey M, Kaskar K, Chappell N, Zhu L, Devlin D, Valdes C, Schutt A, Woodard T, Zarutskie P, Cochran R, Gibbons W. Development of a dynamic machine learning algorithm to predict clinical pregnancy and live birth rate with embryo morphokinetics. F&S Reports 2022, 3: 116-123. PMID: 35789724, PMCID: PMC9250114, DOI: 10.1016/j.xfre.2022.04.004.Peer-Reviewed Original ResearchClinical pregnancy ratePregnancy rateClinical pregnancyAcademic fertility clinicRetrospective cohort analysisLive birth rateTertiary hospital settingLive birth outcomesPositive predictive valueBirth outcomesCohort analysisHospital settingFertility clinicsPredictive valueSecondary analysisPregnancyEmbryo morphokineticsBirth rateMorphokineticsTime-lapse microscopySimilar resultsPatientsClinicDNMT3A Loss Drives Enhancer Hypomethylation in FLT3-ITD-Associated Leukemias
Yang L, Rodriguez B, Mayle A, Park H, Lin X, Luo M, Jeong M, Curry C, Kim S, Ruau D, Zhang X, Zhou T, Zhou M, Rebel V, Challen G, Göttgens B, Lee J, Rau R, Li W, Goodell M. DNMT3A Loss Drives Enhancer Hypomethylation in FLT3-ITD-Associated Leukemias. Cancer Cell 2016, 30: 363-365. PMID: 27505680, DOI: 10.1016/j.ccell.2016.07.015.Peer-Reviewed Original Research
2024
Dose-dependent effects of Dnmt3a in an inducible murine model of Kras(G12D)-driven leukemia.
Rogers JH, Rosen A, Reyes JM, Ketkar S, Conneely SE, Gupta R, Yang L, Miller MB, Medrano G, Aguilar R, Uchenda N, Goodell MA, Rau RE. Dose-dependent effects of Dnmt3a in an inducible murine model of Kras(G12D)-driven leukemia. Exp Hematol 2024, 135: 104248. PMID: 38834136, DOI: 10.1016/j.exphem.2024.104248.Peer-Reviewed Original Research
2023
Small Bowel Obstruction in Postpartum Vaginal Delivery due to Prior Abdominal Adhesions Case Report
Yang L, Kao L. Small Bowel Obstruction in Postpartum Vaginal Delivery due to Prior Abdominal Adhesions Case Report. Case Reports In Obstetrics And Gynecology 2023, 2023: 6563205. PMID: 37025389, PMCID: PMC10072967, DOI: 10.1155/2023/6563205.Peer-Reviewed Case Reports and Technical NotesSmall bowel obstructionUncomplicated vaginal deliveryVaginal deliveryBowel obstructionIntestinal obstructionInitial abdominal X-rayComputed tomography abdomenNonspecific abdominal painAbdominal X-rayPrior appendectomyTomography abdomenAbdominal painMultiparous womenCT abdomenSurgical repairAppendectomy surgeryExam findingsCase reportRisk factorsObstructionAbdomenDeliveryAppendectomyPainSurgery
2021
Fetal programming of polycystic ovary syndrome: Effects of androgen exposure on prenatal ovarian development
Barsky M, Merkison J, Hosseinzadeh P, Yang L, Bruno-Gaston J, Dunn J, Gibbons W, Blesson C. Fetal programming of polycystic ovary syndrome: Effects of androgen exposure on prenatal ovarian development. The Journal Of Steroid Biochemistry And Molecular Biology 2021, 207: 105830. PMID: 33515680, PMCID: PMC8056856, DOI: 10.1016/j.jsbmb.2021.105830.Peer-Reviewed Original ResearchConceptsGene expressionPolycystic ovary syndromeOvarian developmentDifferential gene expressionFetal gonadal developmentDownregulated genesTranscriptional pathwaysCandidate genesMitochondrial studiesMitochondrial oxygen consumptionProton leakOvary syndromeGonadal developmentMitochondrial functionFetal programmingMitochondrial ultrastructureStrong hereditary componentMitochondrial dysfunctionMitochondrial efficiencyMitochondriaAndrogen exposureGenesInheritance patternOocyte maturationAndrogen programming
2020
Embryos from polycystic ovary syndrome patients with hyperandrogenemia reach morula stage faster than controls
Chappell N, Barsky M, Shah J, Peavey M, Yang L, Sangi-Haghpeykar H, Gibbons W, Blesson C. Embryos from polycystic ovary syndrome patients with hyperandrogenemia reach morula stage faster than controls. F&S Reports 2020, 1: 125-132. PMID: 34223228, PMCID: PMC8244380, DOI: 10.1016/j.xfre.2020.05.006.Peer-Reviewed Original ResearchPolycystic ovary syndromeHyperandrogenic polycystic ovary syndromeHigher miscarriage rateMiscarriage rateBody mass index-matched patientsPolycystic ovary syndrome patientsAcademic fertility clinicRetrospective cohort analysisTertiary hospital settingObstetric outcomesRotterdam criteriaControl patientsOvary syndromeAltered fertilitySyndrome patientsCohort analysisHospital settingBlastulation ratePatientsOvarian folliclesFertility clinicsEmbryo morphokineticsEarly embryo developmentWomenFertilization rate
2015
Long Non-Coding RNAs Control Hematopoietic Stem Cell Function
Luo M, Jeong M, Sun D, Park H, Rodriguez B, Xia Z, Yang L, Zhang X, Sheng K, Darlington G, Li W, Goodell M. Long Non-Coding RNAs Control Hematopoietic Stem Cell Function. Cell Stem Cell 2015, 16: 426-438. PMID: 25772072, PMCID: PMC4388783, DOI: 10.1016/j.stem.2015.02.002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Transcription FactorsBinding SitesBone Marrow CellsCell DifferentiationCell LineageCell Self RenewalCells, CulturedDNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenesis, GeneticGene Expression ProfilingGene Expression Regulation, DevelopmentalHematopoietic Stem CellsHigh-Throughput Nucleotide SequencingMiceMice, Inbred StrainsMice, KnockoutRNA, Long NoncodingRNA, Small InterferingConceptsHematopoietic stem cellsLong non-coding RNAsNon-coding RNAsLineage commitmentKey hematopoietic transcription factorsUnique gene expression programProtein-coding genesHematopoietic stem cell functionCell fate decisionsGene expression programsHematopoietic transcription factorsStem cell functionGenetic circuitryDifferentiated lineagesExpression programsFate decisionsLncRNA genesUnannotated lncRNAsDNA methylationEpigenetic featuresTranscription factorsHSC functionDeep sequencingGene expressionRegulated expressionDNMT3A in haematological malignancies
Yang L, Rau R, Goodell M. DNMT3A in haematological malignancies. Nature Reviews Cancer 2015, 15: 152-165. PMID: 25693834, PMCID: PMC5814392, DOI: 10.1038/nrc3895.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHaematopoietic stem cell differentiationStem cell differentiationStem cell programAberrant DNA methylationDominant negative inhibitorImportant biological relationshipsDifferentiated lineagesHistone methyltransferasesDNA methyltransferasesRegulatory domainDNA methylationMechanistic functionGene expressionHSC progenyMethyltransferase 3AImportant new targetHSC expansionCell differentiationUnique mutational profileDNMT3ACell programHuman HSCsStem cellsEnzymatic activityMutationsDnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation
Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry C, Challen G, Li W, Wheeler D, Rebel V, Goodell M. Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation. Blood 2015, 125: 629-638. PMID: 25416277, PMCID: PMC4304108, DOI: 10.1182/blood-2014-08-594648.Peer-Reviewed Original ResearchConceptsHematologic malignanciesDNMT3A mutationsB-cell acute lymphocytic leukemiaAcute lymphocytic leukemiaSpectrum of malignanciesAcute myeloid leukemiaStem cellsPreleukemic phenotypeMyelodysplastic syndromePoor prognosisMyeloid leukemiaLymphocytic leukemiaLymphoid malignanciesMouse modelIrradiated micePrimary myelofibrosisMalignancyPreleukemic cellsLoss of functionDNA methyltransferase 3AMalignant transformationHematopoietic stem cellsMyeloid diseasesLeukemiaLymphoid lineage
2014
Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells
Challen G, Sun D, Mayle A, Jeong M, Luo M, Rodriguez B, Mallaney C, Celik H, Yang L, Xia Z, Cullen S, Berg J, Zheng Y, Darlington G, Li W, Goodell M. Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells. Cell Stem Cell 2014, 15: 350-364. PMID: 25130491, PMCID: PMC4163922, DOI: 10.1016/j.stem.2014.06.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBeta CateninCell DifferentiationCell ProliferationCpG IslandsDNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenesis, GeneticGene Expression Regulation, DevelopmentalGene Regulatory NetworksHematopoietic Stem CellsIsoenzymesMice, Inbred C57BLMice, KnockoutNeoplasmsConceptsHematopoietic stem cellsDe novo methylation patternsAdult hematopoietic stem cellsHSC fate decisionsLoss of Dnmt3bStem cellsΒ-catenin signalingFate decisionsEpigenetic regulationMethylation patternsCpG islandsLifelong productionDistinct functionsHSC differentiationConditional inactivationDNMT3BHSC expansionDifferentiation blockSevere blockDistinct rolesImpaired differentiationDifferentiationMild phenotypeRegulationCells
2013
Large conserved domains of low DNA methylation maintained by Dnmt3a
Jeong M, Sun D, Luo M, Huang Y, Challen G, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Göttgens B, Lee J, Gunaratne P, Godley L, Darlington G, Rao A, Li W, Goodell M. Large conserved domains of low DNA methylation maintained by Dnmt3a. Nature Genetics 2013, 46: 17-23. PMID: 24270360, PMCID: PMC3920905, DOI: 10.1038/ng.2836.Peer-Reviewed Original ResearchMeSH Keywords5-MethylcytosineAnimalsBase SequenceConserved SequenceCpG IslandsCytosineDatabases, GeneticDNA (Cytosine-5-)-MethyltransferasesDNA MethylationDNA Methyltransferase 3AEpigenesis, GeneticGene Expression RegulationHematopoietic Stem CellsHistonesHumansLeukemiaMaleMiceMice, Inbred C57BLMice, Mutant Strains
2012
Less Is More: Unveiling the Functional Core of Hematopoietic Stem Cells through Knockout Mice
Rossi L, Lin K, Boles N, Yang L, King K, Jeong M, Mayle A, Goodell M. Less Is More: Unveiling the Functional Core of Hematopoietic Stem Cells through Knockout Mice. Cell Stem Cell 2012, 11: 302-317. PMID: 22958929, PMCID: PMC3461270, DOI: 10.1016/j.stem.2012.08.006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHematopoietic stem cellsHSC functionSomatic stem cell typesCell cycle controlStem cellsPTEN/AKTKey regulatory pathwaysStem cell typesTGF-β signalingKnockout miceHSC biologyRegulatory pathwaysCycle controlGenetic analysisCell typesFunctional coreFunctional modulesCellsWntCohesive pictureGenesSignalingBiologyAktMice
2008
Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia
Guller S, Buhimschi CS, Y Y, Huang T, Yang L, Kuczynski E, Zambrano E, Lockwood CJ, Buhimschi IA. Placental expression of ceruloplasmin in pregnancies complicated by severe preeclampsia. Laboratory Investigation 2008, 88: 1057-1067. PMID: 18679377, PMCID: PMC2682720, DOI: 10.1038/labinvest.2008.74.Peer-Reviewed Original ResearchConceptsPlasminogen activator inhibitor-1Severe preeclampsiaReperfusion injurySFlt-1Soluble fms-like tyrosine kinase-1Fms-like tyrosine kinase-1Ischemia/reperfusion injuryLife-threatening syndromeSubsequent reperfusion injuryTerm control groupPathophysiology of preeclampsiaTyrosine kinase-1Activator inhibitor-1Release of factorsSignificant increasePresence of mRNAUpregulation of mRNACeruloplasmin mRNAPlacental damagePlacental factorsMicroarray gene profilingEndothelium dysfunctionPE placentasQuantitative real-time PCRIntervillous space
2005
Expression and Regulation of Glucocorticoid Receptor in Human Placental Villous Fibroblasts
Lee MJ, Wang Z, Yee H, Ma Y, Swenson N, Yang L, Kadner SS, Baergen RN, Logan SK, Garabedian MJ, Guller S. Expression and Regulation of Glucocorticoid Receptor in Human Placental Villous Fibroblasts. Endocrinology 2005, 146: 4619-4626. PMID: 16055431, DOI: 10.1210/en.2005-0235.Peer-Reviewed Original ResearchConceptsGC receptorSmooth muscle cellsHuman placentaGR levelsHormone treatmentShort-term GC treatmentMuscle cellsLong-term hormone treatmentVascular smooth muscle cellsGR protein expressionGR mRNA levelsPlacental cell typesNormal term human placentasTotal GR levelsTerm human placentaCell typesGR antibodyGC treatmentHormone exposureStem villiCytotrophoblast cellsGlucocorticoid receptorPlacental fibroblastsTerminal villiVillous fibroblasts