2020
Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2
Rampello AJ, Laudermilch E, Vishnoi N, Prophet SM, Shao L, Zhao C, Lusk CP, Schlieker C. Torsin ATPase deficiency leads to defects in nuclear pore biogenesis and sequestration of MLF2. Journal Of Cell Biology 2020, 219: e201910185. PMID: 32342107, PMCID: PMC7265317, DOI: 10.1083/jcb.201910185.Peer-Reviewed Original ResearchConceptsNuclear pore biogenesisMyeloid leukemia factor 2FG nucleoporinsNuclear pore complex biogenesisNuclear envelope herniationsNuclear envelope reformationLive cell imaging platformProteomics-based approachNuclear envelope blebbingComplex biogenesisBleb formationUbiquitin conjugationNuclear envelopeATPase deficiencyBiogenesisHallmark phenotypePhenotypic hallmarksPOM121Factor 2Late markersLuminal componentsNup358CellsUbiquitinBlebbing
2012
Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination
Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 5311-5315. PMID: 22431626, PMCID: PMC3325651, DOI: 10.1073/pnas.1119262109.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyOne-color excitationWide field natureDiffraction-limited resolutionLiquid crystal devicesSpecial fluorophoresCrystal devicesSIM setupExcitation wavelengthAcquisition speedHigh frame rateLight intensityFrame rateBiological structuresLive-cell imagingMultiple colorsMicroscopyHigh light intensityWavelengthLive cellsExcitationResolutionImaging