Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination
Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 5311-5315. PMID: 22431626, PMCID: PMC3325651, DOI: 10.1073/pnas.1119262109.Peer-Reviewed Original ResearchConceptsStructured-illumination microscopyOne-color excitationWide field natureDiffraction-limited resolutionLiquid crystal devicesSpecial fluorophoresCrystal devicesSIM setupExcitation wavelengthAcquisition speedHigh frame rateLight intensityFrame rateBiological structuresLive-cell imagingMultiple colorsMicroscopyHigh light intensityWavelengthLive cellsExcitationResolutionImaging