2012
25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophages
Xu L, Shen S, Ma Y, Kim JK, Rodriguez-Agudo D, Heuman DM, Hylemon PB, Pandak WM, Ren S. 25-Hydroxycholesterol-3-sulfate attenuates inflammatory response via PPARγ signaling in human THP-1 macrophages. AJP Endocrinology And Metabolism 2012, 302: e788-e799. PMID: 22275753, PMCID: PMC3330710, DOI: 10.1152/ajpendo.00337.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Inflammatory AgentsBlotting, WesternCell NucleusCholesterol EstersCytokinesCytosolEnzyme-Linked Immunosorbent AssayHumansHydroxycholesterolsHypoglycemic AgentsI-kappa B ProteinsLipopolysaccharidesMacrophagesNF-kappa BPPAR gammaProtein TransportReal-Time Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingRosiglitazoneSignal TransductionThiazolidinedionesConceptsNF-κB protein levelsPeroxisome proliferator-activated receptorInflammatory responseNuclear PPARγReporter gene assayLipid metabolismNuclear receptor peroxisome proliferator-activated receptorIL-1β expressionLiver X receptor ligandsProtein levelsActivation of PPARγHuman THP-1 macrophagesProliferator-activated receptorGene assayLuciferase activityTHP-1 macrophagesPPARγ-specific antagonistIκB/NF-κBX receptor ligandsIL-1βPromoter reporter gene assaysCholesterol metabolitesPPARγ activityPotent regulatory moleculesNF-κB
2010
Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells
Bai Q, Xu L, Kakiyama G, Runge-Morris MA, Hylemon PB, Yin L, Pandak WM, Ren S. Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells. Atherosclerosis 2010, 214: 350-356. PMID: 21146170, PMCID: PMC3031658, DOI: 10.1016/j.atherosclerosis.2010.11.021.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedCholesterol EstersChromatography, High Pressure LiquidEndothelial CellsFatty Acids, NonesterifiedGene Expression RegulationHumansHydrocarbons, FluorinatedHydroxycholesterolsLiver X ReceptorsOrphan Nuclear ReceptorsRNA, MessengerSignal TransductionSterol Regulatory Element Binding Protein 1SulfonamidesSulfotransferasesTime FactorsTransfectionTriglyceridesConceptsHuman aortic endothelial cellsLipid metabolismAortic endothelial cellsSULT2B1b overexpressionSREBP-1cEndothelial cellsIntracellular lipid homeostasisOxysterol sulfotransferaseReceptor agonistLipid levelsPresence of T0901317Cellular lipid levelsLipid homeostasisRecombinant adenovirusACC-1Protein levelsSULT2B1bSulfated metabolitesMetabolismCellular lipidsOverexpressionKey regulatorSimilar resultsCellsRegulation of Hepatocyte Lipid Metabolism and Inflammatory Response by 25‐Hydroxycholesterol and 25‐Hydroxycholesterol‐3‐sulfate
Xu L, Bai Q, Rodriguez‐Agudo D, Hylemon PB, Heuman DM, Pandak WM, Ren S. Regulation of Hepatocyte Lipid Metabolism and Inflammatory Response by 25‐Hydroxycholesterol and 25‐Hydroxycholesterol‐3‐sulfate. Lipids 2010, 45: 821-832. PMID: 20700770, DOI: 10.1007/s11745-010-3451-y.Peer-Reviewed Original ResearchConceptsSREBP-1 protein levelsAcetyl-CoA carboxylase 1Fatty acid synthaseLipid metabolismPromoter-reporter geneProtein levelsImportant regulatory moleculesNFkappaB-dependent genesHepatocyte lipid metabolismLXR/SREBPTarget genesFAS mRNA levelsReporter geneRegulatory moleculesKey enzymeNFkappaB nuclear translocationAcid synthasePrimary rat hepatocytesIkappaBalpha levelsCholesterol biosynthesisNuclear translocationGenesLuciferase activityPotent regulatorNFkappaB activation