2017
An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons
Zhang Y, Ni W, Horwich AL, Kaczmarek LK. An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons. Journal Of Neuroscience 2017, 37: 2258-2265. PMID: 28119399, PMCID: PMC5338764, DOI: 10.1523/jneurosci.3102-16.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaBiophysicsCells, CulturedElectric StimulationEnzyme InhibitorsGanglia, InvertebrateHumansLuminescent ProteinsMembrane PotentialsMicroinjectionsMorpholinosMutationNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedRNA, Small InterferingSodiumSuperoxide Dismutase-1ConceptsAmyotrophic lateral sclerosisSuperoxide dismutase 1Mutant superoxide dismutase 1Potassium currentC-Jun N-terminal kinaseNeuronal excitabilityLateral sclerosisFatal adult-onset neurodegenerative diseaseN-terminal kinaseMutant human Cu/ZnNeuronal developmentDismutase 1Adult-onset neurodegenerative diseaseCurrent-clamp recordingsMotor neuron toxicityOutward potassium currentHuman Cu/ZnWild-type superoxide dismutase 1Neuron toxicityActivity of NaBag cell neuronsClamp recordingsNeuronal functionCell neuronsAction potentials
2010
The Slack Sodium-Activated Potassium Channel Provides a Major Outward Current in Olfactory Neurons of Kv1.3−/− Super-Smeller Mice
Lu S, Das P, Fadool DA, Kaczmarek LK. The Slack Sodium-Activated Potassium Channel Provides a Major Outward Current in Olfactory Neurons of Kv1.3−/− Super-Smeller Mice. Journal Of Neurophysiology 2010, 103: 3311-3319. PMID: 20393063, PMCID: PMC2888249, DOI: 10.1152/jn.00607.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysicsCardiovascular AgentsCells, CulturedElectric StimulationGene Expression RegulationIn Vitro TechniquesKv1.3 Potassium ChannelMembrane PotentialsMiceMice, Inbred C57BLMice, KnockoutNerve Tissue ProteinsNeuronsOlfactory BulbPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedPyrimidinesRNA InterferenceSodium Channel BlockersTetrodotoxinTransfectionConceptsMitral cellsOlfactory bulbOutward currentsOlfactory neuronsWildtype animalsPotassium channelsMajor outward currentVoltage-clamp recordingsVoltage-dependent potassium channelsNet outward currentIntracellular sodiumOB slicesPotassium channel genesCompensatory increaseFiring patternsWestern blottingRNA interference approachPrimary culturesEnhanced expressionDetection of odorsSame treatmentChannel genesMiceNeuronsOlfactory phenotypes
2007
Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission
Hickman JA, Hardwick JM, Kaczmarek LK, Jonas EA. Bcl-xL Inhibitor ABT-737 Reveals a Dual Role for Bcl-xL in Synaptic Transmission. Journal Of Neurophysiology 2007, 99: 1515-1522. PMID: 18160428, PMCID: PMC2836590, DOI: 10.1152/jn.00598.2007.Peer-Reviewed Original ResearchConceptsMitochondrial outer membraneEndogenous Bcl-xLMitochondrial channel activityBcl-xLInhibitor ABT-737ABT-737Outer membraneBcl-xL.Pro-apoptotic cleavage productRecombinant Bcl-xLChannel activityBcl-xL proteinSynaptic functionDual roleGenetic toolsDomain pocketSynaptic transmissionSynaptic activityGiant presynaptic terminalEquivalent modificationEndogenous proteolysisRepetitive synaptic activityBH3Cleavage productsProteinSlack and Slick KNa Channels Regulate the Accuracy of Timing of Auditory Neurons
Yang B, Desai R, Kaczmarek LK. Slack and Slick KNa Channels Regulate the Accuracy of Timing of Auditory Neurons. Journal Of Neuroscience 2007, 27: 2617-2627. PMID: 17344399, PMCID: PMC6672517, DOI: 10.1523/jneurosci.5308-06.2007.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAnimals, NewbornAuditory PathwaysBithionolBrain StemComputer SimulationElectric ConductivityElectric StimulationElectrophysiologyIn Vitro TechniquesMiceModels, NeurologicalNerve Tissue ProteinsNeuronsNeurons, AfferentPotassium ChannelsPotassium Channels, Sodium-ActivatedReaction TimeSodium
2006
Pharmacological activation and inhibition of Slack (Slo2.2) channels
Yang B, Gribkoff VK, Pan J, Damagnez V, Dworetzky SI, Boissard CG, Bhattacharjee A, Yan Y, Sigworth FJ, Kaczmarek LK. Pharmacological activation and inhibition of Slack (Slo2.2) channels. Neuropharmacology 2006, 51: 896-906. PMID: 16876206, DOI: 10.1016/j.neuropharm.2006.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Infective Agents, LocalBepridilBithionolCalcium Channel BlockersCell Line, TransformedDose-Response Relationship, DrugDose-Response Relationship, RadiationElectric StimulationEnzyme ActivationEnzyme InhibitorsHumansMembrane PotentialsOocytesPatch-Clamp TechniquesPotassium Channels, Calcium-ActivatedQuinidineTransfectionXenopusConceptsSlack channelsConcentration-dependent mannerIschemic injuryPharmacological activationKNa channelsMammalian brainFiring ratePharmacological propertiesChannel subunitsReversible increaseChannel activityCell linesBepridilHEK cellsRobust activatorNeuronsStable cell linesInhibitionExcised patchesXenopus oocytesPresent studyBithionolChannel openingSpecific roleMembrane patches
2005
Acoustic environment determines phosphorylation state of the Kv3.1 potassium channel in auditory neurons
Song P, Yang Y, Barnes-Davies M, Bhattacharjee A, Hamann M, Forsythe ID, Oliver DL, Kaczmarek LK. Acoustic environment determines phosphorylation state of the Kv3.1 potassium channel in auditory neurons. Nature Neuroscience 2005, 8: 1335-1342. PMID: 16136041, DOI: 10.1038/nn1533.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic StimulationAction PotentialsAnimalsAnimals, NewbornBrain StemCHO CellsCricetinaeCricetulusDose-Response Relationship, RadiationElectric StimulationEnzyme InhibitorsFunctional LateralityGene Expression RegulationImmunohistochemistryIn Vitro TechniquesIndolesMaleimidesNeuronsPatch-Clamp TechniquesPhosphorylationProtein Kinase CRatsRats, Sprague-DawleyTetradecanoylphorbol AcetateConceptsKv3.1 potassium channelAction potentialsAuditory neuronsPotassium channelsShort-duration action potentialsRat brainstem neuronsAuditory brainstem nucleiProtein kinase CHigh-frequency spikingHigh-frequency auditoryBrainstem neuronsHigh-frequency stimuliBrainstem nucleiSynaptic stimulationNeuronsInteraural differencesSound localizationIntrinsic electrical propertiesKinase C
2004
Kv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics
Fadool DA, Tucker K, Perkins R, Fasciani G, Thompson RN, Parsons AD, Overton JM, Koni PA, Flavell RA, Kaczmarek LK. Kv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics. Neuron 2004, 41: 389-404. PMID: 14766178, PMCID: PMC2737549, DOI: 10.1016/s0896-6273(03)00844-4.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAdaptor Proteins, Vesicular TransportAnimalsBehavior, AnimalBlotting, WesternBody WeightBrain-Derived Neurotrophic FactorCalcium ChannelsCells, CulturedDensitometryDifferential ThresholdDiscrimination, PsychologicalDose-Response Relationship, DrugDrinkingElectric StimulationEmbryo, MammalianEnergy IntakeExploratory BehaviorGene DeletionGRB10 Adaptor ProteinHabituation, PsychophysiologicHumansInsulinKidneyKineticsKv1.3 Potassium ChannelMembrane PotentialsMiceMice, KnockoutMotor ActivityNerve Tissue ProteinsNeuronsNeurotoxinsNuclear Matrix-Associated ProteinsOdorantsOlfactory BulbPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Voltage-GatedProteinsRas ProteinsReceptor, trkBReverse Transcriptase Polymerase Chain ReactionRNA, MessengerScorpion VenomsSensory ThresholdsSrc-Family KinasesTime FactorsTyrosine 3-MonooxygenaseConceptsKv1.3-/- miceProtein-protein interactionsGene-targeted deletionKv1.3-null miceSignal transductionScaffolding proteinSignaling cascadesChannel genesC-type inactivationDeletionMembrane potentialNull miceOlfactory codingDetection of odorsPotassium channelsKv1.3 channelsProteinSense of smellSlow inactivation kineticsWild-type miceTransductionGenesOlfactory bulb mitral cellsMiceRole
2003
Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL
Jonas EA, Hoit D, Hickman JA, Brandt TA, Polster BM, Fannjiang Y, McCarthy E, Montanez MK, Hardwick JM, Kaczmarek LK. Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL. Journal Of Neuroscience 2003, 23: 8423-8431. PMID: 12968005, PMCID: PMC6740692, DOI: 10.1523/jneurosci.23-23-08423.2003.Peer-Reviewed Original ResearchConceptsBcl-2 family proteinsProtein Bcl-xLBcl-xLFamily proteinsMitochondrial membranePro-apoptotic cleavage productRecombinant Bcl-xLBcl-xL proteinMitochondrial calcium uptakePresynaptic terminalsInfluences synaptic transmissionCell deathGiant presynaptic terminalSynaptic transmissionChannel activityProteinSquid stellate ganglionMitochondriaCleavage productsSynaptic stabilityAdult brainPostsynaptic responsesCalcium uptakeMembranePatch pipette
2002
Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons
Zhang Y, Magoski NS, Kaczmarek LK. Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons. Journal Of Neuroscience 2002, 22: 10134-10141. PMID: 12451114, PMCID: PMC6758731, DOI: 10.1523/jneurosci.22-23-10134.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCells, CulturedElectric StimulationEnzyme ActivatorsEnzyme InhibitorsLarge-Conductance Calcium-Activated Potassium ChannelsNeural InhibitionNeuronsPatch-Clamp TechniquesPhloretinPotassiumPotassium Channel BlockersPotassium Channels, Calcium-ActivatedProtein Kinase CRefractory Period, ElectrophysiologicalSphingosineTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsRefractory periodBK currentsProtein kinase COnset of afterdischargeBK channel activityApplication of phloretinBK channel activatorsProlonged refractory periodAbility of stimulationRole of Ca2Blocker paxillinePharmacological characteristicsChannel activatorIntracellular Ca2Prolonged increaseOutward currentsInhibitor of PKCAfterdischargesNeuronsAdditional stimulationProlonged activationActivator of PKCChannel activity
2001
Casein Kinase 2 Determines the Voltage Dependence of the Kv3.1 Channel in Auditory Neurons and Transfected Cells
Macica C, Kaczmarek L. Casein Kinase 2 Determines the Voltage Dependence of the Kv3.1 Channel in Auditory Neurons and Transfected Cells. Journal Of Neuroscience 2001, 21: 1160-1168. PMID: 11160386, PMCID: PMC6762230, DOI: 10.1523/jneurosci.21-04-01160.2001.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAuditory PathwaysBinding SitesBrain StemCasein Kinase IICDC2-CDC28 KinasesCHO CellsCricetinaeCyclin-Dependent Kinase 2Cyclin-Dependent KinasesElectric StimulationEnzyme InhibitorsIn Vitro TechniquesMembrane PotentialsNeuronsNeuropeptidesPatch-Clamp TechniquesPhosphorylationPotassium ChannelsPotassium Channels, Voltage-GatedPrecipitin TestsProtein Kinase CProtein Serine-Threonine KinasesRatsShaw Potassium ChannelsTetradecanoylphorbol AcetateTransfectionConceptsCasein kinase 2Kinase 2Casein kinase IIProtein kinase CKv3.1 channelsChinese hamster ovary cellsHamster ovary cellsConstitutive phosphorylationPhosphatase treatmentKinase IIKinase CTransfected CellsVoltage-dependent activationOvary cellsWhole-cell conductancePhosphorylationPotassium channelsRectifier channelsBiophysical characteristicsInactivationKv3.1 potassium channelVoltage dependenceActivationKv3.1Patch-clamp recordings
2000
Activation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones
Magoski N, Knox R, Kaczmarek L. Activation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones. The Journal Of Physiology 2000, 522: 271-283. PMID: 10639103, PMCID: PMC2269759, DOI: 10.1111/j.1469-7793.2000.t01-2-00271.x.Peer-Reviewed Original ResearchConceptsVoltage-gated Ca2Non-selective cation channelsRefractory periodCation channelsElectrical stimulationFree salineAbility of nifedipinePresence of nifedipinePresence of TTXIntact abdominal gangliaProlonged refractory periodOnset of refractorinessAfferent inputBrief synaptic stimulationHigh external potassiumPermeable cation channelIntracellular calciumAbdominal ganglionExtracellular Ca2Synaptic stimulationAfterdischargesNormal responseIntracellular Ca2Intracellular storesRepeated stimulation
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1998
Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia
Whim M, Kaczmarek L. Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia. Neuroscience Letters 1998, 258: 143-146. PMID: 9885951, DOI: 10.1016/s0304-3940(98)00850-7.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAplysiaCells, CulturedCycloleucineElectric StimulationGTP-Binding ProteinsNeuronsReceptors, Metabotropic GlutamateConceptsBag cell neuronsCell neuronsMetabotropic glutamate receptorsG proteinsPeptidergic bag cell neuronsAfferent stimulationSpontaneous firingAfferent inputGlutamate receptorsSecond messenger pathwaysPharmacological activationReceptor activationAfterdischargesNeuronsMessenger pathwaysReceptorsActivationAdditional pathwaysExpressionExcitabilityPathwaySustained periodContribution of the Kv3.1 potassium channel to high‐frequency firing in mouse auditory neurones
Wang L, Gan L, Forsythe I, Kaczmarek L. Contribution of the Kv3.1 potassium channel to high‐frequency firing in mouse auditory neurones. The Journal Of Physiology 1998, 509: 183-194. PMID: 9547392, PMCID: PMC2230948, DOI: 10.1111/j.1469-7793.1998.183bo.x.Peer-Reviewed Original Research
1993
Inward rectification of the minK potassium channel
Blumenthal E, Kaczmarek L. Inward rectification of the minK potassium channel. The Journal Of Membrane Biology 1993, 136: 23-29. PMID: 8271270, DOI: 10.1007/bf00241486.Peer-Reviewed Original ResearchThe peptide FMRFa terminates a discharge in Aplysia bag cell neurons by modulating calcium, potassium, and chloride conductances
Fisher T, Lin C, Kaczmarek L. The peptide FMRFa terminates a discharge in Aplysia bag cell neurons by modulating calcium, potassium, and chloride conductances. Journal Of Neurophysiology 1993, 69: 2164-2173. PMID: 7688803, DOI: 10.1152/jn.1993.69.6.2164.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalcium ChannelsCells, CulturedChloride ChannelsElectric StimulationElectrophysiologyFMRFamideGangliaImmunohistochemistryIon ChannelsMembrane PotentialsMembrane ProteinsNeuritesNeuronsNeuropeptidesNeurotransmitter AgentsPotassium ChannelsStereotyped BehaviorTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsAction potentialsElectrical stimulationVoltage-activated calcium currentsOnset of afterdischargePowerful inhibitory influenceIntact abdominal gangliaIon substitution experimentsVoltage-clamp experimentsAfferent nervesProtein kinase C. 5Channel blockersCalcium currentPrimary cell culturesAbdominal ganglionInhibitory influenceAfterdischargesCyclic AMP analogueFMRFaOutward currentsNeuronal processesNeuronsAplysia bag cell neuronsReversal potential
1992
Inhibition of peptide release from invertebrate neurons by the protein kinase inhibitor H-7
Loechner K, Mattessich-Arrandale J, Azhderian E, Kaczmarek L. Inhibition of peptide release from invertebrate neurons by the protein kinase inhibitor H-7. Brain Research 1992, 581: 315-318. PMID: 1393536, DOI: 10.1016/0006-8993(92)90724-n.Peer-Reviewed Original Research
1991
Dexamethasone increases potassium channel messenger RNA and activity in clonal pituitary cells.
Levitan E, Hemmick L, Birnberg N, Kaczmarek L. Dexamethasone increases potassium channel messenger RNA and activity in clonal pituitary cells. Endocrinology 1991, 5: 1903-8. PMID: 1791837, DOI: 10.1210/mend-5-12-1903.Peer-Reviewed Original ResearchConceptsPituitary cellsChannel expressionClonal rat pituitary cellsChannel messenger RNAIon channel expressionRat pituitary cellsDose-dependent mannerClonal pituitary cellsAnterior pituitaryLong-term effectsRat hypothalamusSimilar pharmacologyGlucocorticoid agonistGlucocorticoid hormonesChannel mRNAGH3 cellsElectrical activitySecretionSteady-state concentrationsTerm effectsPituitaryExcitable cellsHormoneIon channelsMessenger RNA
1990
Progressive potentiation of peptide release during a neuronal discharge
Loechner K, Azhderian E, Dreyer R, Kaczmarek L. Progressive potentiation of peptide release during a neuronal discharge. Journal Of Neurophysiology 1990, 63: 738-744. PMID: 2341872, DOI: 10.1152/jn.1990.63.4.738.Peer-Reviewed Original Research
1987
Alpha bag cell peptide directly modulates the excitability of the neurons that release it
Kauer J, Fisher T, Kaczmarek L. Alpha bag cell peptide directly modulates the excitability of the neurons that release it. Journal Of Neuroscience 1987, 7: 3623-3632. PMID: 2824716, PMCID: PMC6569037, DOI: 10.1523/jneurosci.07-11-03623.1987.Peer-Reviewed Original ResearchConceptsAlpha-bag cell peptideBag cell neuronsAlpha-BCPCell neuronsBag cell peptidesPotassium currentCell peptidesVoltage-dependent potassium currentsCAMP levelsVoltage-dependent outward currentIntact abdominal gangliaBag cell clustersAdenylate cyclase activator forskolinAbility of forskolinCyclase activator forskolinLevels of cAMPVoltage-clamp experimentsElectrophysiological actionsIntracellular cAMP levelsImmunohistochemical stainingIntact gangliaAbdominal ganglionPhosphodiesterase inhibitorPharmacological elevationOutward currents