2011
AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦
Zhang L, Jouret F, Rinehart J, Sfakianos J, Mellman I, Lifton RP, Young LH, Caplan MJ. AMP-activated Protein Kinase (AMPK) Activation and Glycogen Synthase Kinase-3β (GSK-3β) Inhibition Induce Ca2+-independent Deposition of Tight Junction Components at the Plasma Membrane* ♦. Journal Of Biological Chemistry 2011, 286: 16879-16890. PMID: 21383016, PMCID: PMC3089531, DOI: 10.1074/jbc.m110.186932.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsCadherinsCalciumCell AdhesionCell MembraneDogsEpitheliumGene Expression Regulation, EnzymologicGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaMembrane ProteinsMicroscopy, FluorescencePhosphoproteinsPhosphorylationRNA InterferenceTight JunctionsZonula Occludens-1 ProteinConceptsProtein kinase activationTight junction componentsJunction componentsPlasma membraneAMPK activationKinase activationGSK-3β inhibitionNectin-afadin systemEpithelial tight junctionsTight junctionsPhosphorylation studiesSynthase kinaseJunctional proteinsAbsence of extracellularDistinct pathwaysCell growthE-cadherinIndependent depositionKinaseActivationInduce Ca2MembraneAfadinExtracellularInhibition
2006
AMP-activated protein kinase regulates the assembly of epithelial tight junctions
Zhang L, Li J, Young LH, Caplan MJ. AMP-activated protein kinase regulates the assembly of epithelial tight junctions. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 17272-17277. PMID: 17088526, PMCID: PMC1859922, DOI: 10.1073/pnas.0608531103.Peer-Reviewed Original ResearchConceptsTight junction assemblyJunction assemblyProtein kinaseLKB1-dependent phosphorylationCell polarization processCellular energy statusActivation of AMPKTight junctionsEukaryotic cellsTight junction structureAMPKMDCK cellsEpithelial tight junctionsEnergy statusKinaseEpithelial cellsAbsence of Ca2AssemblyTransepithelial electrical resistanceParacellular fluxZonula occludens-1CellsRecent studiesOccludens-1LKB1
2002
Correlation of myocardial p-(123)I-iodophenylpentadecanoic acid retention with (18)F-FDG accumulation during experimental low-flow ischemia.
Shi CQ, Young LH, Daher E, DiBella EV, Liu YH, Heller EN, Zoghbi S, Wackers FJ, Soufer R, Sinusas AJ. Correlation of myocardial p-(123)I-iodophenylpentadecanoic acid retention with (18)F-FDG accumulation during experimental low-flow ischemia. Journal Of Nuclear Medicine 2002, 43: 421-31. PMID: 11884504.Peer-Reviewed Original Research
1998
Additive Effects of Hyperinsulinemia and Ischemia on Myocardial GLUT1 and GLUT4 Translocation In Vivo
Russell R, Yin R, Caplan M, Hu X, Ren J, Shulman G, Sinusas A, Young L. Additive Effects of Hyperinsulinemia and Ischemia on Myocardial GLUT1 and GLUT4 Translocation In Vivo. Circulation 1998, 98: 2180-2186. PMID: 9815873, DOI: 10.1161/01.cir.98.20.2180.Peer-Reviewed Original Research
1997
Low-flow ischemia leads to translocation of canine heart GLUT-4 and GLUT-1 glucose transporters to the sarcolemma in vivo.
Young L, Renfu Y, Russell R, Hu X, Caplan M, Ren J, Shulman G, Sinusas A. Low-flow ischemia leads to translocation of canine heart GLUT-4 and GLUT-1 glucose transporters to the sarcolemma in vivo. Circulation 1997, 95: 415-22. PMID: 9008459, DOI: 10.1161/01.cir.95.2.415.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportDogsFluorescent Antibody TechniqueGlucose Transporter Type 1Glucose Transporter Type 4HeartIntracellular MembranesMonosaccharide Transport ProteinsMuscle ProteinsMyocardial IschemiaMyocardiumRegional Blood FlowSarcolemmaSubcellular FractionsTissue Distribution
1996
Glucose metabolism distal to a critical coronary stenosis in a canine model of low-flow myocardial ischemia.
McNulty PH, Sinusas AJ, Shi CQ, Dione D, Young LH, Cline GC, Shulman GI. Glucose metabolism distal to a critical coronary stenosis in a canine model of low-flow myocardial ischemia. Journal Of Clinical Investigation 1996, 98: 62-69. PMID: 8690805, PMCID: PMC507401, DOI: 10.1172/jci118778.Peer-Reviewed Original ResearchConceptsLow-flow ischemiaCoronary stenosisIschemic myocardiumIschemic regionLow-flow myocardial ischemiaCritical coronary stenosisExogenous glucoseOxidative substrateMyocardial ischemiaIntact dogsGlucose metabolismNonischemic regionsClinical observationsCanine modelGlucose utilizationIschemiaBiochemical measurementsMyocardial regionsRate of incorporationIntracellular metabolismTricarboxylic acid cycle fluxStenosisMyocardiumGlucoseGlucose-derived pyruvate
1995
Technetium-99m-nitroimidazole (BMS181321): a positive imaging agent for detecting myocardial ischemia.
Shi CQ, Sinusas AJ, Dione DP, Singer MJ, Young LH, Heller EN, Rinker BD, Wackers FJ, Zaret BL. Technetium-99m-nitroimidazole (BMS181321): a positive imaging agent for detecting myocardial ischemia. Journal Of Nuclear Medicine 1995, 36: 1078-86. PMID: 7769431.Peer-Reviewed Original ResearchConceptsIschemic regionMyocardial ischemiaBlood flowEx vivo SPECTRegional myocardial blood flowPartial coronary occlusionMicrosphere blood flowMyocardial blood flowOpen-chest canine modelVivo SPECT imagesSPECT imagesVivo planarCentral ischemic regionCoronary occlusionDemand ischemiaBMS181321Technetium-99mIntravenous injectionLiver ratioMyocardial imagingCanine modelPositive imagingHepatic clearanceLiver activityArterial sampling
1992
Physiological hyperinsulinemia inhibits myocardial protein degradation in vivo in the canine heart.
Young LH, Dahl DM, Rauner D, Barrett EJ. Physiological hyperinsulinemia inhibits myocardial protein degradation in vivo in the canine heart. Circulation Research 1992, 71: 393-400. PMID: 1628395, DOI: 10.1161/01.res.71.2.393.Peer-Reviewed Original ResearchConceptsMyocardial protein degradationProtein degradationProtein synthesisMyocardial protein turnoverAmino acid replacementsAcid replacementsProtein turnoverEssential amino acidsAmino acidsPhenylalanine balanceAmino acid concentrationsNet myocardial releaseSpecific activityPhenylalanineVivoDegradationAnimalsBasal rateIsotopic steady statePhysiological hyperinsulinemiaTurnover
1990
In vivo measurement of myocardial protein turnover using an indicator dilution technique.
Revkin JH, Young LH, Stirewalt WS, Dahl DM, Gelfand RA, Zaret BL, Barrett EJ. In vivo measurement of myocardial protein turnover using an indicator dilution technique. Circulation Research 1990, 67: 902-912. PMID: 2208613, DOI: 10.1161/01.res.67.4.902.Peer-Reviewed Original Research
1989
Physiologic hyperinsulinemia stimulates lactate extraction by heart muscle in the conscious dog
Young L, Zaret B, Barrett E. Physiologic hyperinsulinemia stimulates lactate extraction by heart muscle in the conscious dog. Metabolism 1989, 38: 1115-1119. PMID: 2682138, DOI: 10.1016/0026-0495(89)90049-8.Peer-Reviewed Original ResearchConceptsPhysiologic hyperinsulinemiaConscious dogsFree fatty acidsArterial free fatty acid concentrationMumol/minHeart muscleArterial plasma insulinMyocardial lactate uptakeFree fatty acid concentrationsArterial lactate concentrationHyperinsulinemic-euglycemic clampMyocardial glucose uptakeHyperinsulinemia increasesEuglycemic hyperinsulinemiaNormal heart muscleEuglycemic clampFatty acidsPlasma insulinBasal measurementsBlood glucoseLactate extractionFatty acid concentrationsMyocardial uptakeLactate uptakeHyperinsulinemia
1988
Effect of Chronic Diabetes on Myocardial Fuel Metabolism and Insulin Sensitivity
Barrett E, Schwartz R, Young L, Jacob R, Zaret B. Effect of Chronic Diabetes on Myocardial Fuel Metabolism and Insulin Sensitivity. Diabetes 1988, 37: 943-948. PMID: 3290011, DOI: 10.2337/diab.37.7.943.Peer-Reviewed Original ResearchConceptsArterial free fatty acidsFree fatty acidsMyocardial balanceInsulin clampBranched-chain amino acid concentrationsEuglycemic insulin clamp techniqueGlucose uptakeInsulin-induced hypoaminoacidemiaInsulin clamp techniqueInsulin-deficient diabetesPlasma glucose concentrationFuel substrate metabolismSignificant myocardial uptakeEffect of insulinMyocardial fuel metabolismSignificant glucose uptakePhysiologic hyperinsulinemiaBasal periodChronic diabetesDiabetic animalsInsulin sensitivityAcute effectsAdditional dogsAmino acid concentrationsMyocardial uptake
1987
An isotopic method for measurement of muscle protein synthesis and degradation in vivo
Barrett EJ, Revkin JH, Young LH, Zaret BL, Jacob R, Gelfand RA. An isotopic method for measurement of muscle protein synthesis and degradation in vivo. Biochemical Journal 1987, 245: 223-228. PMID: 3663148, PMCID: PMC1148103, DOI: 10.1042/bj2450223.Peer-Reviewed Original ResearchConceptsNet amino acid releaseFemoral venous plasmaTissue blood flowAmino acid releaseMuscle protein synthesisHindlimb flowTissue protein degradationSpecific radioactivityContinuous infusionUptake of leucineMuscle bedArterial circulationClinical studiesPlasma concentrationsDog hindlimbBlood flowProtein synthesisAcid releaseHindlimbProtein turnoverTissue proteinsPhenylalanine incorporationRemoval of tracerMuscle tissueDogsMyocardial amino acid transport by canine sarcolemma vesicles
Young LH, Zaret BL, Barrett EJ. Myocardial amino acid transport by canine sarcolemma vesicles. American Journal Of Physiology 1987, 252: h1070-h1076. PMID: 3035946, DOI: 10.1152/ajpheart.1987.252.6.h1070.Peer-Reviewed Original ResearchConceptsCanine ventricular myocardiumVentricular myocardiumSarcoplasmic reticulumSarcolemma vesiclesLeucine uptakeAmino acid uptakeL-leucineDependent alanine transport activityAmino acid transportX minStimulatory effectPmol xAcid uptakeMammalian heartSaturable functionMyocardiumAcid transportSarcolemmaLeucine transportAlanine transport activityAlanine fluxMembrane vesiclesDifferent membrane preparations