2017
An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells
Julia T, Wang M, Pimenova A, Bowles K, Hartley B, Lacin E, Machlovi S, Abdelaal R, Karch C, Phatnani H, Slesinger P, Zhang B, Goate A, Brennand K. An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Reports 2017, 9: 600-614. PMID: 28757165, PMCID: PMC5550034, DOI: 10.1016/j.stemcr.2017.06.018.Peer-Reviewed Original Research
2016
Neural organoids for disease phenotyping, drug screening and developmental biology studies
Hartley B, Brennand K. Neural organoids for disease phenotyping, drug screening and developmental biology studies. Neurochemistry International 2016, 106: 85-93. PMID: 27744003, PMCID: PMC5389930, DOI: 10.1016/j.neuint.2016.10.004.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHuman induced pluripotent stem cellsNeural organoidsCell-extracellular matrix interactionsCell typesDevelopmental biology studiesSpecific physiological functionsCell-cell interactionsInduced pluripotent stem cellsNervous system cell typesCentral nervous system cell typesPluripotent stem cellsCell replacement therapyBiology studiesCurrent biomedical researchDifferentiation protocolsPhysiological functionsComplex tissuesMatrix interactionsNovel technological platformStem cellsDisease mechanismsSpatial organizationUnknown disease mechanismsToxicity assaysHeterogeneous tissues
2015
Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells
Ho S, Hartley B, Julia T, Beaumont M, Stafford K, Slesinger P, Brennand K. Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells. Methods 2015, 101: 113-124. PMID: 26626326, PMCID: PMC4860098, DOI: 10.1016/j.ymeth.2015.11.019.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsNeural progenitor cellsHiPSC-derived neural progenitor cellsHigh-throughput drug screeningHiPSC neural progenitor cellsExogenous transcription factorsProgenitor cellsInduced pluripotent stem cellsPatient-specific platformPluripotent stem cellsPatient-derived neuronsSomatic reprogrammingTranscription factorsGenetic variationExcitatory neuronsDrug screeningNeurogenin 2Neuronal inductionFunctional neuronsThroughput drug screeningNeuronal phenotypeLentiviral transductionStem cellsStarting populationDisease etiology
2010
High-Efficient Generation of Induced Pluripotent Stem Cells from Human Astrocytes
Ruiz S, Brennand K, Panopoulos A, Herrerías A, Gage F, Izpisua-Belmonte J. High-Efficient Generation of Induced Pluripotent Stem Cells from Human Astrocytes. PLOS ONE 2010, 5: e15526. PMID: 21170306, PMCID: PMC3000364, DOI: 10.1371/journal.pone.0015526.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsHuman somatic cell typesEmbryoid body generationHuman embryonic stem cellsEmbryonic germ layersEmbryonic stem cellsStem cellsInduced pluripotent stem cellsTeratoma formationRegenerative medicineSomatic cell typesPluripotent markersReprogramming efficiencyHuman somatic cellsHiPS cellsHigh-efficient generationGerm layersAutologous cellsEfficiencySomatic cellsSimilar efficiencyPluripotencyNeural cell typesCell typesLayer