Factor VIII organisation on nanodiscs with different lipid composition
Grushin K, Miller J, Dalm D, Stoilova-McPhie S. Factor VIII organisation on nanodiscs with different lipid composition. Thrombosis And Haemostasis 2015, 113: 741-749. PMID: 25589466, DOI: 10.1160/th14-09-0725.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsBinding SitesFactor VIIIImage Processing, Computer-AssistedImaging, Three-DimensionalMembrane ProteinsMembranes, ArtificialMicroscopy, Electron, TransmissionNanostructuresPhosphatidylserinesProtein BindingProtein ConformationProtein FoldingProtein MultimerizationRecombinant ProteinsSurface PropertiesSwineConceptsMembrane-bound factor VIIISingle-particle electron microscopyProtein-membrane interfaceElectron microscopyComplex assemblyBinding of FVIIIFVIII formsSingle-particle reconstructionDifferent lipid compositionsFunctional studiesNanodiscsProteinLipid compositionPhysiological conditionsFurther assemblyPhosphatidylserineStructural studiesMembrane patchesMembranePlatelet membranesStructure determinationNormal haemostasisParticle reconstructionComplexesAssembly