2017
Reversible stacking of lipid nanodiscs for structural studies of clotting factors
Grushin K, White M, Stoilova-McPhie S. Reversible stacking of lipid nanodiscs for structural studies of clotting factors. Nanotechnology Reviews 2017, 6: 139-148. DOI: 10.1515/ntrev-2016-0073.Peer-Reviewed Original ResearchMembrane scaffold proteinND populationMembrane-associated proteinsDifferent membrane scaffold proteinsSize of nanodiscsDiscoidal phospholipid bilayerLipid nanodiscsLipid ratioCryo-EMBiophysical studiesMacromolecular compositionFunctional studiesNanodiscsLipid compositionStructural studiesPhospholipid bilayersProteinMillimolar concentrationsCa2Lipid mixturesMSP1D1ND formMicroscopy studiesElectron microscopy studiesPopulation
2015
Factor VIII organisation on nanodiscs with different lipid composition
Grushin K, Miller J, Dalm D, Stoilova-McPhie S. Factor VIII organisation on nanodiscs with different lipid composition. Thrombosis And Haemostasis 2015, 113: 741-749. PMID: 25589466, DOI: 10.1160/th14-09-0725.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsBinding SitesFactor VIIIImage Processing, Computer-AssistedImaging, Three-DimensionalMembrane ProteinsMembranes, ArtificialMicroscopy, Electron, TransmissionNanostructuresPhosphatidylserinesProtein BindingProtein ConformationProtein FoldingProtein MultimerizationRecombinant ProteinsSurface PropertiesSwineConceptsMembrane-bound factor VIIISingle-particle electron microscopyProtein-membrane interfaceElectron microscopyComplex assemblyBinding of FVIIIFVIII formsSingle-particle reconstructionDifferent lipid compositionsFunctional studiesNanodiscsProteinLipid compositionPhysiological conditionsFurther assemblyPhosphatidylserineStructural studiesMembrane patchesMembranePlatelet membranesStructure determinationNormal haemostasisParticle reconstructionComplexesAssembly