2024
Single-Cell Analysis Reveals a Subset of High IL-12p40-Secreting Dendritic Cells within Mouse Bone Marrow-Derived Macrophages Differentiated with M-CSF.
Bridges K, Pizzurro G, Khunte M, Chen M, Salvador Rocha E, Alexander A, Bass V, Kellman L, Baskaran J, Miller-Jensen K. Single-Cell Analysis Reveals a Subset of High IL-12p40-Secreting Dendritic Cells within Mouse Bone Marrow-Derived Macrophages Differentiated with M-CSF. The Journal Of Immunology 2024, 212: 1357-1365. PMID: 38416039, DOI: 10.4049/jimmunol.2300431.Peer-Reviewed Original ResearchBone marrow-derived macrophagesDendritic cellsCell-to-cell heterogeneitySingle-cell RNA sequencing dataRNA sequencing dataSingle-cell analysisIL-12p40Sequence dataExpression of IL12BInnate immune functionProduction of IL-12Bone marrow-derived macrophage culturesMurine bone marrow-derived macrophagesSurface marker expressionAcute inflammatory responseMarrow-derived macrophagesGene encoding IL-12p40Secretion assayIL12B expressionReporter miceDC lineageIL-12GenesProinflammatory cytokinesM-CSF
2023
Combinatorial Immunotherapy with Agonistic CD40 Activates Dendritic Cells to Express IL12 and Overcomes PD-1 Resistance.
Krykbaeva I, Bridges K, Damsky W, Pizzurro G, Alexander A, McGeary M, Park K, Muthusamy V, Eyles J, Luheshi N, Turner N, Weiss S, Olino K, Kaech S, Kluger H, Miller-Jensen K, Bosenberg M. Combinatorial Immunotherapy with Agonistic CD40 Activates Dendritic Cells to Express IL12 and Overcomes PD-1 Resistance. Cancer Immunology Research 2023, 11: 1332-1350. PMID: 37478171, DOI: 10.1158/2326-6066.cir-22-0699.Peer-Reviewed Original ResearchConceptsPD-1 resistanceDendritic cellsTumor regressionAnti-PD-1 resistanceActivates Dendritic CellsCytokine secretion profilingSystemic cytokine profileTriple therapy combinationInnate immune activationAdaptive immune responsesComplete tumor regressionMajority of miceSignificant clinical challengeMouse melanoma modelT cell activationAgonistic CD40Checkpoint inhibitorsDC subsetsTriple therapyCytokine profileImmune activationCombinatorial immunotherapyTherapy combinationsT cellsClinical challenge