2000
Nuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*
Yao G, Sun B, Insogna K, Weir E. Nuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*. Endocrinology 2000, 141: 2914-2922. PMID: 10919279, DOI: 10.1210/endo.141.8.7592.Peer-Reviewed Original ResearchConceptsCSF-1 expressionGene expressionElectrophoretic mobility shift assaysCSF-1 promoterCSF-1 gene expressionMobility shift assaysInducible complexTranscriptional mechanismsShift assaysNuclear factor-κB p50Northern analysisNF-kappaB p50NF-kappaB siteCSF-1Messenger RNAC-RelBcl-3Rel BRNA expressionTNF treatmentHematopoietic growth factorsMessenger RNA expressionIkappaB-alphaOsteoblastsNF-kappaB
1998
The Cell-surface Form of Colony-stimulating Factor-1 Is Regulated by Osteotropic Agents and Supports Formation of Multinucleated Osteoclast-like Cells*
Yao G, Sun B, Hammond E, Spencer E, Horowitz M, Insogna K, Weir E. The Cell-surface Form of Colony-stimulating Factor-1 Is Regulated by Osteotropic Agents and Supports Formation of Multinucleated Osteoclast-like Cells*. Journal Of Biological Chemistry 1998, 273: 4119-4128. PMID: 9461606, DOI: 10.1074/jbc.273.7.4119.Peer-Reviewed Original ResearchConceptsColony-stimulating factor-1Osteoclast formationReverse transcriptase-polymerase chain reactionFactor 1Quantitative reverse transcriptase-polymerase chain reactionTumor necrosis factorTranscriptase-polymerase chain reactionOsteoclast-like cellsHematopoietic growth factorsCSF-1 isoformsView of evidenceCell surface formPolymerase chain reactionCo-culture systemNecrosis factorOsteotropic hormonesOsteoclast function
1996
Circulating levels of interleukin-6 and tumor necrosis factor-alpha are elevated in primary hyperparathyroidism and correlate with markers of bone resorption--a clinical research center study
Grey A, Mitnick MA, Shapses S, Ellison A, Gundberg C, Insogna K. Circulating levels of interleukin-6 and tumor necrosis factor-alpha are elevated in primary hyperparathyroidism and correlate with markers of bone resorption--a clinical research center study. The Journal Of Clinical Endocrinology & Metabolism 1996, 81: 3450-3454. PMID: 8855783, DOI: 10.1210/jcem.81.10.8855783.Peer-Reviewed Original ResearchConceptsPrimary hyperparathyroidismUntreated primary hyperparathyroidismSerum type ITumor necrosisInterleukin-6Bone resorptionSerum levelsBiochemical markersSerum deoxypyridinolineUrinary deoxypyridinolineUrinary pyridinolineBone lossCarboxyterminal telopeptideSoluble receptorClinical research center studyControl valuesNormal parathyroid functionSuccessful surgical treatmentBone-resorbing cytokinesInterleukin-1 betaType IIntact PTHParathyroid functionSurgical treatmentCenter studyTumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts
Kaplan D, Eielson C, Horowitz M, Insogna K, Weir E. Tumor necrosis factor‐α induces transcription of the colony‐stimulating factor‐1 gene in murine osteoblasts. Journal Of Cellular Physiology 1996, 168: 199-208. PMID: 8647916, DOI: 10.1002/(sici)1097-4652(199607)168:1<199::aid-jcp24>3.0.co;2-1.Peer-Reviewed Original ResearchConceptsColony-stimulating factor-1CSF-1 expressionMC3T3-E1 cellsCSF-1 mRNACSF-1 gene transcriptionMRNA stability studiesFactor 1 geneNew protein synthesisSecond messenger pathwaysPrimary mouse osteoblastsColony-stimulating factor-1 (CSF-1) geneTranscriptional mechanismsGene transcriptionResponsive cell linesInduces transcriptionNorthern analysisProgenitor proliferationTNF-alpha treatmentProtein synthesisCAMP pathwayTranscript expressionMessenger pathwaysMurine osteoblastsCycloheximide treatmentCellular mechanisms