Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis
Croda J, Figueira CP, Wunder EA, Santos CS, Reis MG, Ko AI, Picardeau M. Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infection And Immunity 2008, 76: 5826-5833. PMID: 18809657, PMCID: PMC2583567, DOI: 10.1128/iai.00989-08.Peer-Reviewed Original ResearchConceptsPathogenic Leptospira speciesLeptospira speciesAcute disease manifestationsPersistent renal colonizationInoculation of ratsDisease manifestationsHamster modelRenal colonizationPathogenic mechanismsAnimal modelsPersistent colonizationDecrease virulenceLeptospira interrogansHomologous recombinationLeptospirosisVirulence factorsBacterial adherenceImmunoblot analysisCorresponding chromosomal locusWild-type strainCultured cellsFirst evidenceSpectinomycin resistance geneRepeat proteinsTargeted Mutagenesis