2023
Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels
Devendrakumar K, Copeland C, Adamchek C, Zhong X, Huang X, Gendron J, Li X. Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels. New Phytologist 2023, 240: 354-371. PMID: 37571862, PMCID: PMC11114105, DOI: 10.1111/nph.19187.Peer-Reviewed Original ResearchConceptsTubby-like proteinsN-terminal F-box domainF-box E3 ligase complexesImmunoprecipitation-mass spectrometry analysisArabidopsis Immune ResponsesPlant immune responsesProtein levelsDomain-containing proteinsF-box domainF-box proteinsE3 ligase complexProteasome-dependent mannerRedundant homologsOverexpression linesTubby domainUbiquitination substratesSkp1-CullinTLP functionsLigase complexTubby proteinBiosynthesis enzymesDouble mutantMass spectrometry analysisProtein 6Novel mechanism
2021
Unique N-Terminal Interactions Connect F-BOX STRESS INDUCED (FBS) Proteins to a WD40 Repeat-like Protein Pathway in Arabidopsis
Sepulveda-Garcia E, Fulton EC, Parlan EV, O’Connor L, Fleming AA, Replogle AJ, Rocha-Sosa M, Gendron JM, Thines B. Unique N-Terminal Interactions Connect F-BOX STRESS INDUCED (FBS) Proteins to a WD40 Repeat-like Protein Pathway in Arabidopsis. Plants 2021, 10: 2228. PMID: 34686037, PMCID: PMC8537223, DOI: 10.3390/plants10102228.Peer-Reviewed Original ResearchFBX proteinsSCF complexSCF-type E3 ubiquitin ligasesProtein targetsF-box domainF-box proteinsProtein degradation eventsE3 ubiquitin ligasesFBS proteinsC-terminal domainSkp proteinInteracting proteinSubstrate adaptorUbiquitin ligasesDomain interactsEnvironmental stressBiological functionsC-terminusN-terminusFacilitate recruitmentProtein pathwayFB proteinNuclear eventsArabidopsisProtein
2018
Decoys Untangle Complicated Redundancy and Reveal Targets of Circadian Clock F-Box Proteins
Lee CM, Feke A, Li MW, Adamchek C, Webb K, Pruneda-Paz J, Bennett EJ, Kay SA, Gendron JM. Decoys Untangle Complicated Redundancy and Reveal Targets of Circadian Clock F-Box Proteins. Plant Physiology 2018, 177: 1170-1186. PMID: 29794020, PMCID: PMC6052990, DOI: 10.1104/pp.18.00331.Peer-Reviewed Original ResearchConceptsLOV KELCH PROTEIN2Target proteinsCircadian clockEukaryotic circadian clocksF-box proteinsE3 ubiquitin ligasesUbiquitin-proteasome systemDominant-negative formImmunoprecipitation-mass spectrometryMutant plantsF-BOX1Genetic redundancyChe proteinsKELCH REPEATClock proteinsUbiquitin ligasesZEITLUPEFlowering timeFKF1Proteasome systemFlavin bindingBiochemical roleProteinLight conditionsUbiquitylation