2021
Genes related to SNPs identified by Genome-wide association studies of age-related hearing loss show restriction to specific cell types in the adult mouse cochlea
Xue N, Song L, Song Q, Santos-Sacchi J, Wu H, Navaratnam D. Genes related to SNPs identified by Genome-wide association studies of age-related hearing loss show restriction to specific cell types in the adult mouse cochlea. Hearing Research 2021, 410: 108347. PMID: 34536825, DOI: 10.1016/j.heares.2021.108347.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCochleaGenome-Wide Association StudyHair Cells, AuditoryMicePolymorphism, Single NucleotidePresbycusisConceptsSingle-cell RNA sequencingApical cell polarityGenome-wide association studiesAdult mouse cochleaSpecific cell typesCell polaritySubtypes of cellsSignificant genetic componentSingle nucleotide polymorphismsVesicle recyclingRNA sequencingAssociation studiesGenesVarying ExpressionCell typesGenetic componentNucleotide polymorphismsSNPsMouse cochleaCell functionGenesetsHair cellsHair cell functionSignificant overlapExpression
2020
Functional, Morphological, and Evolutionary Characterization of Hearing in Subterranean, Eusocial African Mole-Rats
Pyott SJ, van Tuinen M, Screven LA, Schrode KM, Bai JP, Barone CM, Price SD, Lysakowski A, Sanderford M, Kumar S, Santos-Sacchi J, Lauer AM, Park TJ. Functional, Morphological, and Evolutionary Characterization of Hearing in Subterranean, Eusocial African Mole-Rats. Current Biology 2020, 30: 4329-4341.e4. PMID: 32888484, PMCID: PMC8109146, DOI: 10.1016/j.cub.2020.08.035.Peer-Reviewed Original ResearchConceptsHair bundle morphologyAmino acid substitutionsAltered hearingLink proteinAcid substitutionsUnique evolutionary trajectoryMolecular evolutionary analysisCochlear amplificationEvolutionary characterizationEvolutionary analysisHuman hearing lossEvolutionary trajectoriesBundle morphologyPrestin functionAfrican Mole-RatsCodon levelSelection pressureSubterranean lifestyleSubterranean rodentsMole-RatUnique lifestyleProteinHair cellsSpeciesOuter hair cellsCalcium‐induced calcium release in proximity to hair cell BK channels revealed by PKA activation
Bai J, Xue N, Lawal O, Nyati A, Santos‐Sacchi J, Navaratnam D. Calcium‐induced calcium release in proximity to hair cell BK channels revealed by PKA activation. Physiological Reports 2020, 8: e14449. PMID: 32748549, PMCID: PMC7399380, DOI: 10.14814/phy2.14449.Peer-Reviewed Original ResearchConceptsBK channelsBK currentsCalcium-activated potassium channelsLarge-conductance calcium-activated potassium channelsCalcium-induced calcium releaseEntry of CaSlo subunitSynaptic releaseCalcium releaseChicken hair cellsPotassium channelsHair cellsIP3 receptorCICRA ActivationPKA activationActivationCritical roleImagingReleaseRyanodine
2016
Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels
Lv C, Stewart WJ, Akanyeti O, Frederick C, Zhu J, Santos-Sacchi J, Sheets L, Liao JC, Zenisek D. Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels. Cell Reports 2016, 15: 2784-2795. PMID: 27292637, PMCID: PMC5334794, DOI: 10.1016/j.celrep.2016.05.045.Peer-Reviewed Original ResearchConceptsNeuromast hair cellsProper synaptic localizationHair cellsSynaptic ribbonsZebrafish genesProper localizationCalcium channelsSynaptic vesiclesSmall vesiclesEnhanced exocytosisFrameshift mutationRibeye proteinsSynaptic localizationVesiclesRelease sitesCellsRibeyeRibbon-like structuresNon-spiking cellsSensory systemsLocalizationGenesExocytosisSimilar numberProtein
2013
Patch-Clamp Recordings from Lateral Line Neuromast Hair Cells of the Living Zebrafish
Ricci AJ, Bai JP, Song L, Lv C, Zenisek D, Santos-Sacchi J. Patch-Clamp Recordings from Lateral Line Neuromast Hair Cells of the Living Zebrafish. Journal Of Neuroscience 2013, 33: 3131-3134. PMID: 23407967, PMCID: PMC3684625, DOI: 10.1523/jneurosci.4265-12.2013.Peer-Reviewed Original ResearchConceptsHair cellsSynaptic vesicle releaseNeuromast hair cellsLiving zebrafishBiological discoveryIonic channel activityZebrafishVesicle releaseChannel activityPhysiological activityLateral lineJunctional couplingCellsDetailed understandingPatch-clamp recordingsVestibular peripheryFishNeuromastsActivityDiscoveryFirst recording
2011
Calcium-Dependent Synaptic Vesicle Trafficking Underlies Indefatigable Release at the Hair Cell Afferent Fiber Synapse
Schnee ME, Santos-Sacchi J, Castellano-Muñoz M, Kong JH, Ricci AJ. Calcium-Dependent Synaptic Vesicle Trafficking Underlies Indefatigable Release at the Hair Cell Afferent Fiber Synapse. Neuron 2011, 70: 326-338. PMID: 21521617, PMCID: PMC3254016, DOI: 10.1016/j.neuron.2011.01.031.Peer-Reviewed Original ResearchGene Expression Gradients along the Tonotopic Axis of the Chicken Auditory Epithelium
Frucht CS, Uduman M, Kleinstein SH, Santos-Sacchi J, Navaratnam DS. Gene Expression Gradients along the Tonotopic Axis of the Chicken Auditory Epithelium. Journal Of The Association For Research In Otolaryngology 2011, 12: 423-435. PMID: 21399991, PMCID: PMC3123449, DOI: 10.1007/s10162-011-0259-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCalcium ChannelsChickensCyclic AMP-Dependent Protein KinasesGene Expression ProfilingGene Expression Regulation, DevelopmentalHair Cells, AuditoryLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsMicroRNAsModels, AnimalOrgan of CortiProtein Kinase CSodium ChannelsConceptsGene expression gradientsAuditory epitheliumKinases protein kinase CChicken auditory epitheliumExpression gradientsAvian auditory epitheliumBasilar papillaFold-change cutoffActivity of kinasesIon channel genesProtein kinase CChicken basilar papillaGene setsGenetic basisEnrichment analysisExpression patternsGene expressionGeneSpring softwareChannel genesMechanism of inductionKinase CGenesMicroarray dataDifferential activityQuantitative PCRMicroRNA181a plays a key role in hair cell regeneration in the avian auditory epithelium
Frucht CS, Santos-Sacchi J, Navaratnam DS. MicroRNA181a plays a key role in hair cell regeneration in the avian auditory epithelium. Neuroscience Letters 2011, 493: 44-48. PMID: 21316421, PMCID: PMC3065390, DOI: 10.1016/j.neulet.2011.02.017.Peer-Reviewed Original ResearchConceptsHair cell regenerationHair cell deathAuditory epitheliumChicken auditory epitheliumCell deathNon-mammalian vertebratesAvian auditory epitheliumCell regenerationHair cellsBasilar papillaNew hair cellsChicken basilar papillaAvian counterpartsOtotoxic injuryCaspase-3MiRNACellsMiR181aKey roleExplantsProliferationRegenerationBrdU-positive cellsVertebratesMammals
2010
Gene Expression Analysis of Forskolin Treated Basilar Papillae Identifies MicroRNA181a as a Mediator of Proliferation
Frucht CS, Uduman M, Duke JL, Kleinstein SH, Santos-Sacchi J, Navaratnam DS. Gene Expression Analysis of Forskolin Treated Basilar Papillae Identifies MicroRNA181a as a Mediator of Proliferation. PLOS ONE 2010, 5: e11502. PMID: 20634979, PMCID: PMC2901389, DOI: 10.1371/journal.pone.0011502.Peer-Reviewed Original ResearchConceptsNew hair cellsAuditory epitheliumChicken auditory epitheliumHair cellsInner ear developmentHair cell regenerationGene expression analysisAuditory hair cellsEar developmentExpression analysisMyosin VIEnrichment analysisCycle controlGene expressionMolecular eventsSingle microRNAMediator of proliferationRelevant pathwaysFunctional experimentsPost-hatch chicksRegenerating tissueMammalsGenesBrdU incorporationCell regenerationThe remarkable cochlear amplifier
Ashmore J, Avan P, Brownell W, Dallos P, Dierkes K, Fettiplace R, Grosh K, Hackney C, Hudspeth A, Jülicher F, Lindner B, Martin P, Meaud J, Petit C, Santos-Sacchi J, Sacchi J, Canlon B. The remarkable cochlear amplifier. Hearing Research 2010, 266: 1-17. PMID: 20541061, PMCID: PMC6366996, DOI: 10.1016/j.heares.2010.05.001.Peer-Reviewed Original Research
1992
On the frequency limit and phase of outer hair cell motility: effects of the membrane filter
Santos-Sacchi J. On the frequency limit and phase of outer hair cell motility: effects of the membrane filter. Journal Of Neuroscience 1992, 12: 1906-1916. PMID: 1578277, PMCID: PMC6575887, DOI: 10.1523/jneurosci.12-05-01906.1992.Peer-Reviewed Original Research
1991
Reversible inhibition of voltage-dependent outer hair cell motility and capacitance
Santos-Sacchi J. Reversible inhibition of voltage-dependent outer hair cell motility and capacitance. Journal Of Neuroscience 1991, 11: 3096-3110. PMID: 1941076, PMCID: PMC6575435, DOI: 10.1523/jneurosci.11-10-03096.1991.Peer-Reviewed Original Research
1989
Asymmetry in voltage-dependent movements of isolated outer hair cells from the organ of Corti
Santos-Sacchi J. Asymmetry in voltage-dependent movements of isolated outer hair cells from the organ of Corti. Journal Of Neuroscience 1989, 9: 2954-2962. PMID: 2769373, PMCID: PMC6569687, DOI: 10.1523/jneurosci.09-08-02954.1989.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomechanical PhenomenaCell MovementCell SeparationElectric ConductivityElectrophysiologyGuinea PigsHair Cells, AuditoryOrgan of CortiConceptsOuter hair cellsWhole-cell voltage-clamp techniqueHair cellsNormal resting potentialsOrgan of CortiVoltage-clamp techniqueCell shorteningAcoustic stimulationReceptor potentialResting potentialsResponse magnitudeOHC movementsReported productionStimulationHyperpolarizationComparable levelsNeural tuningCell length changesDepolarizationLength changesCellsBasilar membraneVoltage stimulation
1988
Whole cell currents and mechanical responses of isolated outer hair cells
Santos-Sacchi J, Dilger J. Whole cell currents and mechanical responses of isolated outer hair cells. Hearing Research 1988, 35: 143-150. PMID: 2461927, DOI: 10.1016/0378-5955(88)90113-x.Peer-Reviewed Original Research
1982
Intracellular Recordings from Cochlear Outer Hair Cells
Dallos P, Santos-Sacchi J, Flock Å. Intracellular Recordings from Cochlear Outer Hair Cells. Science 1982, 218: 582-584. PMID: 7123260, DOI: 10.1126/science.7123260.Peer-Reviewed Original Research