2016
Chloride Anions Regulate Kinetics but Not Voltage-Sensor Q max of the Solute Carrier SLC26a5
Santos-Sacchi J, Song L. Chloride Anions Regulate Kinetics but Not Voltage-Sensor Q max of the Solute Carrier SLC26a5. Biophysical Journal 2016, 110: 2551-2561. PMID: 27276272, PMCID: PMC4906268, DOI: 10.1016/j.bpj.2016.05.002.Peer-Reviewed Original Research
2014
Chloride-driven Electromechanical Phase Lags at Acoustic Frequencies Are Generated by SLC26a5, the Outer Hair Cell Motor Protein
Santos-Sacchi J, Song L. Chloride-driven Electromechanical Phase Lags at Acoustic Frequencies Are Generated by SLC26a5, the Outer Hair Cell Motor Protein. Biophysical Journal 2014, 107: 126-133. PMID: 24988347, PMCID: PMC4119270, DOI: 10.1016/j.bpj.2014.05.018.Peer-Reviewed Original ResearchA Genetically-Encoded YFP Sensor with Enhanced Chloride Sensitivity, Photostability and Reduced pH Interference Demonstrates Augmented Transmembrane Chloride Movement by Gerbil Prestin (SLC26a5)
Zhong S, Navaratnam D, Santos-Sacchi J. A Genetically-Encoded YFP Sensor with Enhanced Chloride Sensitivity, Photostability and Reduced pH Interference Demonstrates Augmented Transmembrane Chloride Movement by Gerbil Prestin (SLC26a5). PLOS ONE 2014, 9: e99095. PMID: 24901231, PMCID: PMC4047046, DOI: 10.1371/journal.pone.0099095.Peer-Reviewed Original ResearchChloride and Salicylate Influence Prestin-dependent Specific Membrane Capacitance SUPPORT FOR THE AREA MOTOR MODEL* * This work was supported, in whole or in part, by National Institutes of Health Grant NIDCD DC00273 (to J. S. S.).
Santos-Sacchi J, Song L. Chloride and Salicylate Influence Prestin-dependent Specific Membrane Capacitance SUPPORT FOR THE AREA MOTOR MODEL* * This work was supported, in whole or in part, by National Institutes of Health Grant NIDCD DC00273 (to J. S. S.). Journal Of Biological Chemistry 2014, 289: 10823-10830. PMID: 24554714, PMCID: PMC4036195, DOI: 10.1074/jbc.m114.549329.Peer-Reviewed Original Research
2013
Disparities in voltage-sensor charge and electromotility imply slow chloride-driven state transitions in the solute carrier SLC26a5
Song L, Santos-Sacchi J. Disparities in voltage-sensor charge and electromotility imply slow chloride-driven state transitions in the solute carrier SLC26a5. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 3883-3888. PMID: 23431177, PMCID: PMC3593883, DOI: 10.1073/pnas.1218341110.Peer-Reviewed Original Research
2011
Extracellular chloride regulation of Kv2.1, contributor to the major outward Kv current in mammalian outer hair cells
Li X, Surguchev A, Bian S, Navaratnam D, Santos-Sacchi J. Extracellular chloride regulation of Kv2.1, contributor to the major outward Kv current in mammalian outer hair cells. American Journal Of Physiology - Cell Physiology 2011, 302: c296-c306. PMID: 21940671, PMCID: PMC4054960, DOI: 10.1152/ajpcell.00177.2011.Peer-Reviewed Original Research
2010
Conformational State-Dependent Anion Binding in Prestin: Evidence for Allosteric Modulation
Song L, Santos-Sacchi J. Conformational State-Dependent Anion Binding in Prestin: Evidence for Allosteric Modulation. Biophysical Journal 2010, 98: 371-376. PMID: 20141749, PMCID: PMC2814207, DOI: 10.1016/j.bpj.2009.10.027.Peer-Reviewed Original ResearchAllosteric RegulationAnimalsAnionsCell MembraneCells, CulturedCentral Nervous System AgentsChloridesElectric CapacitanceGuinea PigsHair Cells, Auditory, OuterInhibitory Concentration 50Membrane PotentialsModels, NeurologicalNonlinear DynamicsPatch-Clamp TechniquesProtein ConformationProteinsSalicylatesTemperature
2009
Prestin's Anion Transport and Voltage-Sensing Capabilities Are Independent
Bai JP, Surguchev A, Montoya S, Aronson PS, Santos-Sacchi J, Navaratnam D. Prestin's Anion Transport and Voltage-Sensing Capabilities Are Independent. Biophysical Journal 2009, 96: 3179-3186. PMID: 19383462, PMCID: PMC2718310, DOI: 10.1016/j.bpj.2008.12.3948.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnalysis of VarianceAnimalsAnion Transport ProteinsAntiportersCarbon RadioisotopesChloridesCHO CellsCricetinaeCricetulusElectric CapacitanceFormatesGerbillinaeIon TransportMiceMutation, MissenseOxalatesPatch-Clamp TechniquesSalicylatesSulfate TransportersConceptsClosest phylogenetic relativesTransmembrane regionSLC26 anion transporter familyMammalian outer hair cellsMembrane protein prestinPrestin's motor functionAnion transportPhylogenetic relativesAnion transporter familyTransporter familyProtein prestinChloride-binding siteGating charge movementPrestinCharge movementHair cellsOuter hair cellsResiduesMechanistic conceptsVoltage sensingTransport capabilityCellsVoltage sensorPrevious observationsUptake studies
2006
Control of Mammalian Cochlear Amplification by Chloride Anions
Santos-Sacchi J, Song L, Zheng J, Nuttall AL. Control of Mammalian Cochlear Amplification by Chloride Anions. Journal Of Neuroscience 2006, 26: 3992-3998. PMID: 16611815, PMCID: PMC6673883, DOI: 10.1523/jneurosci.4548-05.2006.Peer-Reviewed Original ResearchOn the temperature and tension dependence of the outer hair cell lateral membrane conductance GmetL and its relation to prestin
Santos-Sacchi J, Rybalchenko V, Bai JP, Song L, Navaratnam D. On the temperature and tension dependence of the outer hair cell lateral membrane conductance GmetL and its relation to prestin. Pflügers Archiv - European Journal Of Physiology 2006, 452: 283. PMID: 16521020, DOI: 10.1007/s00424-005-0037-2.Peer-Reviewed Original Research
2003
Cl− flux through a non‐selective, stretch‐sensitive conductance influences the outer hair cell motor of the guinea‐pig
Rybalchenko V, Santos‐Sacchi J. Cl− flux through a non‐selective, stretch‐sensitive conductance influences the outer hair cell motor of the guinea‐pig. The Journal Of Physiology 2003, 547: 873-891. PMID: 12562920, PMCID: PMC2342734, DOI: 10.1111/j.1469-7793.2003.00873.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarbenoxoloneCell MembraneChloride ChannelsChloridesCyclooxygenase InhibitorsElectric CapacitanceElectric ConductivityGadoliniumGuinea PigsHair Cells, Auditory, OuterIon Channel GatingMechanotransduction, CellularMembrane PotentialsMercuric ChlorideMicrodialysisNiflumic AcidOctanolsProteinsSulfonic AcidsConceptsOuter hair cell motorLateral membranesVoltage-dependent conformational changesLateral plasma membranePlasma membraneMotor proteinsCell motorProtein transitionsConformational changesRecent identificationPrestinSomatic motilityAllosteric modulationCL sensitivityAnion fluxHair cellsCochlear amplificationAcoustic ratesOuter hair cellsAlternative mechanismComplicated roleMembranePronounced shiftCellsAmplification