2014
NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*
Lee C, Liu A, Miranda-Ribera A, Hyun SW, Lillehoj EP, Cross AS, Passaniti A, Grimm PR, Kim BY, Welling PA, Madri JA, DeLisser HM, Goldblum SE. NEU1 Sialidase Regulates the Sialylation State of CD31 and Disrupts CD31-driven Capillary-like Tube Formation in Human Lung Microvascular Endothelia*. Journal Of Biological Chemistry 2014, 289: 9121-9135. PMID: 24550400, PMCID: PMC3979388, DOI: 10.1074/jbc.m114.555888.Peer-Reviewed Original ResearchConceptsHuman pulmonary microvascular ECsCapillary-like tube formationEC tube formationTube formationCell adhesion molecule-1Pulmonary microvascular ECsHuman Lung Microvascular EndotheliaNeu1 sialidaseLung microvascular endotheliumAdhesion molecule-1Endothelial cell adhesion molecule-1Platelet endothelial cell adhesion molecule-1Endothelial cell expressionMultiplicity of infectionMicrovascular endotheliumMolecule-1Microvascular ECsCell expressionCD31Matrigel substrateSialylation statePeanut agglutinin lectinAdhesion moleculesInhibitory effectAngiogenic phenotype
2006
PECAM‐1 modulates thrombin‐induced tissue factor expression on endothelial cells
Zhang JJ, Kelm RJ, Biswas P, Kashgarian M, Madri JA. PECAM‐1 modulates thrombin‐induced tissue factor expression on endothelial cells. Journal Of Cellular Physiology 2006, 210: 527-537. PMID: 17111362, DOI: 10.1002/jcp.20908.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsApoptosisBlood CoagulationCells, CulturedDisease Models, AnimalDown-RegulationEarly Growth Response Protein 1Endothelial CellsFibrinHumansKidneyMaleMAP Kinase Signaling SystemMiceMice, Inbred C57BLMice, KnockoutOligodeoxyribonucleotides, AntisensePlatelet Endothelial Cell Adhesion Molecule-1Receptor, PAR-1Reperfusion InjuryRNA, MessengerThrombinThromboplastinThrombosisConceptsTissue factor expressionHuman umbilical vein endothelial cellsFactor expressionPECAM-1TF inductionEndothelial cellsP38 phosphorylationCell adhesion molecule-1Transient renal ischemiaThrombin receptor PAR-1PAR-1 antagonistsPertussis toxin inhibitionAdhesion molecule-1Endothelial cell adhesion molecule-1Receptor PAR-1PI3K-Akt phosphorylationGalphai/o subunitsPECAM-1 expressionRho-kinase activityUmbilical vein endothelial cellsVein endothelial cellsRenal ischemiaEgr-1 expressionFibrin depositionPlatelet functionPECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation
Biswas P, Canosa S, Schoenfeld D, Schoenfeld J, Li P, Cheas LC, Zhang J, Cordova A, Sumpio B, Madri JA. PECAM-1 Affects GSK-3β-Mediated β-Catenin Phosphorylation and Degradation. American Journal Of Pathology 2006, 169: 314-324. PMID: 16816383, PMCID: PMC1698776, DOI: 10.2353/ajpath.2006.051112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninBlotting, WesternCapillary PermeabilityCells, CulturedEndothelial CellsFluorescent Antibody TechniqueGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHistamineHistamine AgentsHumansMiceModels, BiologicalPhosphatidylinositol 3-KinasesPhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Proto-Oncogene Proteins c-aktReceptors, HistamineSignal TransductionConceptsAdherens junctionsSerine phosphorylationSrc homology 2 domainBeta-catenin expression levelsAdherens junction componentsSerine phosphorylation levelEndothelial cellsΒ-catenin phosphorylationPECAM-1Cell biological responsesCytoplasmic domainSHP-2Proteosomal degradationGSK-3betaDynamic regulatorJunction componentsPhosphorylation levelsPhosphorylationEndothelial cell adhesion molecule-1Expression levelsGSK-3βBiological responsesEndothelial barrier permeabilityMice exhibitCell adhesion molecule-1
2005
Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice
Carrithers M, Tandon S, Canosa S, Michaud M, Graesser D, Madri JA. Enhanced Susceptibility to Endotoxic Shock and Impaired STAT3 Signaling in CD31-Deficient Mice. American Journal Of Pathology 2005, 166: 185-196. PMID: 15632011, PMCID: PMC1602311, DOI: 10.1016/s0002-9440(10)62243-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDisease SusceptibilityDNA-Binding ProteinsEndothelium, VascularFemaleFlow CytometryGene Expression RegulationLipopolysaccharidesMiceMice, Inbred C57BLMice, KnockoutPlatelet Endothelial Cell Adhesion Molecule-1Pulmonary CirculationShock, SepticSpleenSTAT3 Transcription FactorTrans-ActivatorsTumor Necrosis Factor-alphaVanadatesConceptsCD31-deficient miceAcute phase responseSeptic shockEndothelial integritySerum tumor necrosis factor alphaTumor necrosis factor alphaEndothelial cellsCell adhesion molecule-1Necrosis factor alphaAdhesion molecule-1Endothelial cell adhesion molecule-1Wild-type controlsIL-6Endotoxic shockMCP-1Neutrophil transmigrationPhase responseMCP-5Factor alphaImmune stimuliVascular permeabilityInterferon gammaKnockout miceMolecule-1STAT3 Signaling
2003
Abolition of arteriolar dilation but not constriction to histamine in cremaster muscle of eNOS–/– mice
Payne GW, Madri JA, Sessa WC, Segal SS. Abolition of arteriolar dilation but not constriction to histamine in cremaster muscle of eNOS–/– mice. AJP Heart And Circulatory Physiology 2003, 285: h493-h498. PMID: 12689855, DOI: 10.1152/ajpheart.00071.2003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriolesCimetidineEndothelium, VascularHistamineHistamine H1 AntagonistsHistamine H2 AntagonistsMaleMiceMice, Inbred C57BLMice, Inbred StrainsMuscle, SkeletalNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIPlatelet Endothelial Cell Adhesion Molecule-1PyrilamineReceptors, HistamineSignal TransductionVasoconstrictionVasodilationConceptsENOS-/- miceMuscle blood flowVasomotor responsesBlood flowCremaster muscleCell adhesion molecule-1Anesthetized C57Bl6 miceBiphasic vasomotor responseSecond-order arteriolesAdhesion molecule-1Endothelial cell adhesion molecule-1Platelet endothelial cell adhesion molecule-1Nitric oxide releaseTopical histamineConstrictor responsesArteriolar dilationNomega-nitroC57BL6 miceH1 receptorsPharmacological interventionsPermeability of capillariesSmooth muscleMicrovascular endotheliumTissue perfusionCumulative additionLack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis
Solowiej A, Biswas P, Graesser D, Madri JA. Lack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis. American Journal Of Pathology 2003, 162: 953-962. PMID: 12598328, PMCID: PMC1868115, DOI: 10.1016/s0002-9440(10)63890-4.Peer-Reviewed Original ResearchConceptsCell adhesion molecule-1Adhesion molecule-1Endothelial cell adhesion molecule-1Foreign body inflammationBody inflammationMolecule-1Knockout animalsAcute inflammatory modelForeign body implantsAntibody-blocking studiesPECAM-1 knockout micePlatelet endothelial cell adhesion molecule-1PECAM-1 resultsDiminished deliveryNeutrophil accumulationNeutrophil infiltrationLeukocyte accumulationInflammatory modelChronic processDecreased angiogenesisCD31 expressionKnockout miceMice exhibitEndothelial cellsLeukocyte transmigrationElevated glucose inhibits VEGF-A–mediated endocardial cushion formation
Enciso JM, Gratzinger D, Camenisch TD, Canosa S, Pinter E, Madri JA. Elevated glucose inhibits VEGF-A–mediated endocardial cushion formation. Journal Of Cell Biology 2003, 160: 605-615. PMID: 12591918, PMCID: PMC2173755, DOI: 10.1083/jcb.200209014.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis Inducing AgentsAnimalsCell MovementCell SizeCells, CulturedCulture TechniquesDipeptidesEmbryo, MammalianEndocardial Cushion DefectsFemaleGlucoseHeartMaleMatrix Metalloproteinase 2MiceMorphogenesisMyocardiumPlatelet Endothelial Cell Adhesion Molecule-1Protease InhibitorsRecombinant Fusion ProteinsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1ConceptsEpithelial-mesenchymal transformationEndocardial cushion formationPlatelet endothelial cell adhesion molecule-1Cushion formationPECAM-1-positive endothelial cellsSingle cell motilityMMP-2 expressionMorphogenesis resultsHigh glucose-induced inhibitionCell motilityEndothelial cellsBlocks invasionMatrix metalloproteinase-2 expressionEndocardial cellsExtracellular matrixLack of invasionEndothelial cell adhesion molecule-1Mesenchymal cellsMyocardial VEGFMMP-2 inductionMetalloproteinase-2 expressionVEGF-A165ExpressionGrowth factorVascular endothelial growth factor
2002
Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice
Graesser D, Solowiej A, Bruckner M, Osterweil E, Juedes A, Davis S, Ruddle NH, Engelhardt B, Madri JA. Altered vascular permeability and early onset of experimental autoimmune encephalomyelitis in PECAM-1–deficient mice. Journal Of Clinical Investigation 2002, 109: 383-392. PMID: 11827998, PMCID: PMC150854, DOI: 10.1172/jci13595.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisPECAM-1-deficient miceEndothelial cellsAutoimmune encephalomyelitisVascular permeabilityDevelopment of EAET lymphocyte transendothelial migrationEarly onsetHuman autoimmune disease multiple sclerosisAutoimmune disease multiple sclerosisCell adhesion molecule-1Altered vascular permeabilityCNS vascular permeabilityMononuclear cell extravasationDisease multiple sclerosisPlatelet/endothelial cell adhesion molecule-1Wild-type miceAdhesion molecule-1Endothelial cell adhesion molecule-1Subsets of leukocytesPECAM-1 expressionLymphocyte transendothelial migrationEarly time pointsHistamine challengeMultiple sclerosis
1998
Platelet endothelial cell adhesion molecule-1 expression modulates endothelial cell migration in vitro.
Kim C, Wang T, Madri J. Platelet endothelial cell adhesion molecule-1 expression modulates endothelial cell migration in vitro. Laboratory Investigation 1998, 78: 583-90. PMID: 9605183.Peer-Reviewed Original ResearchConceptsWild-type PECAM-1Endothelial cell migrationEndogenous PECAM-1Cell migrationCell-extracellular matrix interactionsMatrix interactionsTyrosine phosphorylation stateMurine conceptusCell-cell adhesionC-myc epitopeIntegrin-extracellular matrix interactionsCell-cell interactionsPECAM-1Endothelial cellsDynamic dephosphorylationCytoskeletal reorganizationEmbryonic vasculogenesisPhosphorylation stateMigratory stateMigration rateRetroviral transductionCultured endothelial cellsEndothelial cell adhesion molecule-1 expressionEndothelial cell adhesion molecule-1Y686
1997
Platelet Endothelial Cell Adhesion Molecule-1 Is Phosphorylatable by c-Src, Binds Src-Src homology 2 Domain, and Exhibits Immunoreceptor Tyrosine-based Activation Motif-like Properties*
Lu T, Barreuther M, Davis S, Madri J. Platelet Endothelial Cell Adhesion Molecule-1 Is Phosphorylatable by c-Src, Binds Src-Src homology 2 Domain, and Exhibits Immunoreceptor Tyrosine-based Activation Motif-like Properties*. Journal Of Biological Chemistry 1997, 272: 14442-14446. PMID: 9162084, DOI: 10.1074/jbc.272.22.14442.Peer-Reviewed Original ResearchConceptsSrc homology 2C-SrcGST-SrcCytoplasmic tailImmunoreceptor tyrosineCytoplasmic domain sequencesEndothelial cell adhesion molecule-1Src SH2 domainCell-cell bordersPECAM-1 functionsPECAM-1PECAM-1 phosphorylationUnidentified phosphoproteinsProtein associatesHomology 2Kinase assaysSignal transductionGlutathione S-transferaseSheet migrationConfluent endothelial cellsActivation motifConsensus sequenceDomain sequencesDomain interactionsEndothelial cells