2023
MicroRNA-1 protects the endothelium in acute lung injury
Korde A, Haslip M, Pednekar P, Khan A, Chioccioli M, Mehta S, Lopez-Giraldez F, Bermejo S, Rojas M, Dela Cruz C, Matthay M, Pober J, Pierce R, Takyar S. MicroRNA-1 protects the endothelium in acute lung injury. JCI Insight 2023, 8: e164816. PMID: 37737266, PMCID: PMC10561733, DOI: 10.1172/jci.insight.164816.Peer-Reviewed Original ResearchConceptsAcute respiratory distress syndromeAcute lung injuryVascular endothelial growth factorAngiopoietin-2Lung injuryAcute injuryMiR-1MicroRNA-1Endothelial cell-specific overexpressionSevere endothelial dysfunctionRespiratory distress syndromeSurvival of miceIntrinsic protective effectContext of injuryCell-specific overexpressionEndothelial growth factorFamily member 3Pneumonia cohortMiR-1 targetsEndothelial dysfunctionDistress syndromeBarrier dysfunctionCapillary leakProtective effectSevere form
1998
Interferon Induction of TAP1
Min W, Pober J, Johnson D. Interferon Induction of TAP1. Circulation Research 1998, 83: 815-823. PMID: 9776728, DOI: 10.1161/01.res.83.8.815.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersDimerizationDNA-Binding ProteinsEndotheliumGene Expression Regulation, EnzymologicGenes, ReporterHeLa CellsHumansInterferon-alphaInterferon-betaInterferon-gammaInterferon-Stimulated Gene Factor 3Interferon-Stimulated Gene Factor 3, gamma SubunitIntracellular Signaling Peptides and ProteinsJanus Kinase 1Janus Kinase 2Major Histocompatibility ComplexPromoter Regions, GeneticProtein Tyrosine Phosphatase, Non-Receptor Type 11Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesProtein-Tyrosine KinasesProto-Oncogene ProteinsRNA, MessengerSH2 Domain-Containing Protein Tyrosine PhosphatasesSignal TransductionSrc Homology DomainsSTAT1 Transcription FactorSTAT2 Transcription FactorTrans-ActivatorsTranscription Factors
1991
MS-1 sinusoidal endothelial antigen is expressed by factor XIIIa+, HLA-DR+ dermal perivascular dendritic cells.
Walsh LJ, Goerdt S, Pober JS, Sueki H, Murphy GF. MS-1 sinusoidal endothelial antigen is expressed by factor XIIIa+, HLA-DR+ dermal perivascular dendritic cells. Laboratory Investigation 1991, 65: 732-41. PMID: 1684403.Peer-Reviewed Original ResearchConceptsPerivascular dendritic cellsDendritic cellsEndothelial cellsInterstitial cellsDendritic interstitial cellsNormal human skinVascular endothelial cellsNonlymphoid organsEndothelial antigensHLA-DRDermal dendrocytesMast cellsImmunofluorescence labeling techniquePerivascular localizationSinusoidal endotheliumPhagocytic macrophagesPerivascular cellsSinusoidal spacesLymphatic endothelial cellsMonoclonal antibodiesBlood vascular endothelial cellsAntibodiesFactor XIIIaBasement membraneDendritic morphology
1990
Cytokines and endothelial cell biology
Pober JS, Cotran RS. Cytokines and endothelial cell biology. Physiological Reviews 1990, 70: 427-451. PMID: 1690903, DOI: 10.1152/physrev.1990.70.2.427.Peer-Reviewed Original Research
1989
Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis.
Munro JM, Pober JS, Cotran RS. Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis. American Journal Of Pathology 1989, 135: 121-33. PMID: 2505619, PMCID: PMC1880213.Peer-Reviewed Original ResearchConceptsLeukocyte accumulationNecrosis factorIFN-gammaEndothelial cell antigen expressionAdhesion moleculesAnti-intercellular adhesion moleculeEndothelial cell hypertrophyPolymorphonuclear leukocyte accumulationTumor necrosis factorCytokine-activated endotheliumLeukocyte adhesion moleculesCell antigen expressionImmune inflammationEndothelial activationLeukocyte infiltrationHypersensitivity reactionsMononuclear cellsHLA-DPEnvelope antigenAntigen expressionVascular permeabilitySubsequent extravasationRecombinant human interferonCell hypertrophyEndothelial morphology
1987
Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture.
Messadi DV, Pober JS, Fiers W, Gimbrone MA, Murphy GF. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture. The Journal Of Immunology 1987, 139: 1557-62. PMID: 3497975, DOI: 10.4049/jimmunol.139.5.1557.Peer-Reviewed Original ResearchConceptsTumor necrosis factorActivation antigensNecrosis factorInterleukin-1Microvascular endotheliumEndothelial cellsEndothelial activation antigenMediators interleukin-1Postcapillary venular endotheliumCultured human umbilical vein endothelial cellsSuperficial vascular plexusOrgan cultureHuman skin organ cultureShort-term organ cultureHuman umbilical vein endothelial cellsSkin organ cultureUmbilical vein endothelial cellsVenular endothelial cellsMicrovascular stainingPostcapillary venular endothelial cellsVein endothelial cellsEndogenous cytokinesLymphocyte traffickingInterleukin-2Venular endotheliumActivation of cultured human endothelial cells by recombinant lymphotoxin: comparison with tumor necrosis factor and interleukin 1 species.
Pober JS, Lapierre LA, Stolpen AH, Brock TA, Springer TA, Fiers W, Bevilacqua MP, Mendrick DL, Gimbrone MA. Activation of cultured human endothelial cells by recombinant lymphotoxin: comparison with tumor necrosis factor and interleukin 1 species. The Journal Of Immunology 1987, 138: 3319-24. PMID: 3494766, DOI: 10.4049/jimmunol.138.10.3319.Peer-Reviewed Original ResearchConceptsTumor necrosis factorAction of lymphotoxinCultured human endothelial cellsHuman endothelial cellsIL-1 speciesPhorbol ester pretreatmentNecrosis factorLeukocyte adhesionEndothelial cellsRecombinant human tumor necrosis factorSecretion of lymphotoxinExpression of HLAIL-1 betaIL-1 alphaInhibited inductionHuman tumor necrosis factorActivated T cellsPhorbol esterInflammatory leukocyte adhesionRecombinant human lymphotoxinInflammatory cellsT cellsIL-1Hr pretreatmentIFN-gammaComposition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells.
Ewenstein BM, Warhol MJ, Handin RI, Pober JS. Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells. Journal Of Cell Biology 1987, 104: 1423-1433. PMID: 3494734, PMCID: PMC2114468, DOI: 10.1083/jcb.104.5.1423.Peer-Reviewed Original ResearchConceptsWeibel-Palade bodiesSecretory organellesUmbilical vein endothelial cellsOrganelle fractionsVein endothelial cellsCultured endothelial cellsIntracellular protein traffickingVon Willebrand factorVon Willebrand factor storageEndothelial cellsSelf-generating Percoll gradientsDirect morphological examinationCultured umbilical vein endothelial cellsUnmodified formProtein traffickingCultured human umbilical vein endothelial cellsRegulated secretionStimulation of ECHuman umbilical vein endothelial cellsStorage organellesOrganellesDense organellesPro sequenceProteolytic processingAdhesive glycoproteinCultured human endothelial cells express platelet-derived growth factor A chain.
Collins T, Pober JS, Gimbrone MA, Hammacher A, Betsholtz C, Westermark B, Heldin CH. Cultured human endothelial cells express platelet-derived growth factor A chain. American Journal Of Pathology 1987, 126: 7-12. PMID: 2433948, PMCID: PMC1899550.Peer-Reviewed Original ResearchConceptsRNA speciesSame relative abundanceHuman endothelial cellsRelative abundanceB polypeptide chainsEndothelial cellsChain transcriptsC-sis geneB-chain transcriptsPlatelet-derived growth factorExtensive structural dataTranscript speciesCultured human umbilical vein endothelial cellsB-chain expressionBovine aortic endothelial cellsHuman umbilical vein endothelial cellsDimeric proteinUmbilical vein endothelial cellsCultured vascular endothelial cellsDifferent genesPrincipal cell typesHybridization analysisPolypeptide chainVein endothelial cellsCell types
1986
Two monokines, interleukin 1 and tumor necrosis factor, render cultured vascular endothelial cells susceptible to lysis by antibodies circulating during Kawasaki syndrome.
Leung DY, Geha RS, Newburger JW, Burns JC, Fiers W, Lapierre LA, Pober JS. Two monokines, interleukin 1 and tumor necrosis factor, render cultured vascular endothelial cells susceptible to lysis by antibodies circulating during Kawasaki syndrome. Journal Of Experimental Medicine 1986, 164: 1958-1972. PMID: 3491174, PMCID: PMC2188474, DOI: 10.1084/jem.164.6.1958.Peer-Reviewed Original ResearchConceptsAcute Kawasaki syndromeTumor necrosis factorKawasaki syndromeHuman endothelial cellsIL-1Cytotoxic antibodiesImmune activationEndothelial cellsNecrosis factorTarget antigenComplement-dependent cytotoxic activityAcute febrile illnessEndothelial cell antigensVascular smooth muscle cellsAge-matched controlsMonocytes/macrophagesSmooth muscle cellsCultured vascular endothelial cellsVascular endothelial cellsComplement-mediated killingDiffuse vasculitisConvalescent phaseFebrile illnessAcute phaseVascular injuryOverlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon.
Pober JS, Gimbrone MA, Lapierre LA, Mendrick DL, Fiers W, Rothlein R, Springer TA. Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. The Journal Of Immunology 1986, 137: 1893-6. PMID: 3091693, DOI: 10.4049/jimmunol.137.6.1893.Peer-Reviewed Original ResearchConceptsTumor necrosis factorIFN-gammaHuman umbilical vein endothelialNecrosis factorIL-1Interleukin-1HUVE cellsImmune interferonIntercellular adhesion molecule-1Endothelial cellsDifferent immune mediatorsAdhesion molecule-1Murine monoclonal antibodiesHuman endothelial cellsImmune mediatorsPattern of activationActivation antigensAntigenic modulationB antigensDifferent lymphokinesMolecule-1Monoclonal antibodiesProgressive increaseInterferonAntigenInduction and detection of a human endothelial activation antigen in vivo.
Cotran RS, Gimbrone MA, Bevilacqua MP, Mendrick DL, Pober JS. Induction and detection of a human endothelial activation antigen in vivo. Journal Of Experimental Medicine 1986, 164: 661-666. PMID: 3723080, PMCID: PMC2188244, DOI: 10.1084/jem.164.2.661.Peer-Reviewed Original ResearchConceptsEndothelial activation antigenActivation antigensSequential skin biopsiesStrong positive stainingHuman umbilical vein endothelial cell culturesEndothelial cell functionLymph nodesHypersensitivity reactionsSkin biopsiesInflammatory reactionImmunoperoxidase techniqueVascular endotheliumPositive stainingEndothelial cell culturesNormal endotheliumMurine mAbsUseful markerH4/18EndotheliumAntigenCell functionSkinPathological tissuesHuman skinTissueRecombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1.
Bevilacqua MP, Pober JS, Majeau GR, Fiers W, Cotran RS, Gimbrone MA. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 4533-4537. PMID: 3487091, PMCID: PMC323768, DOI: 10.1073/pnas.83.12.4533.Peer-Reviewed Original ResearchConceptsRecombinant tumor necrosis factorCultured human vascular endotheliumTumor necrosis factorIL-1Procoagulant activityHuman vascular endotheliumNecrosis factorInterleukin-1Vascular endotheliumTissue factor-like procoagulant activityInduction of PCANatural human interleukin-1Human recombinant tumor necrosis factorEndothelial cell damageCellular procoagulant activityEndothelial procoagulant activityRecombinant IL-1Specific neutralizing antiseraHuman interleukin-1Effects of TNFInitiation of coagulationIliac arteryMaximal dosesPCA responseSaphenous veinImmunoglobulin M antibodies present in the acute phase of Kawasaki syndrome lyse cultured vascular endothelial cells stimulated by gamma interferon.
Leung DY, Collins T, Lapierre LA, Geha RS, Pober JS. Immunoglobulin M antibodies present in the acute phase of Kawasaki syndrome lyse cultured vascular endothelial cells stimulated by gamma interferon. Journal Of Clinical Investigation 1986, 77: 1428-1435. PMID: 3084559, PMCID: PMC424542, DOI: 10.1172/jci112454.Peer-Reviewed Original ResearchConceptsAcute Kawasaki syndromeKawasaki syndromeImmunoglobulin M antibodiesGamma-IFNVein endothelial cellsM antibodiesEndothelial cellsT cellsKS seraGamma interferonClass II major histocompatibility complex antigen expressionMajor histocompatibility complex antigen expressionComplement-dependent cytotoxic activityAnti-endothelial cell antibodiesHUVE cellsHuman dermal fibroblastsHuman saphenous vein smooth muscle cellsSaphenous vein smooth muscle cellsMarked immune activationSecrete gamma interferonSecretion of lymphokinesEndothelial cell antigensAge-matched controlsVein smooth muscle cellsActivated T cellsRecombinant tumor necrosis factor and immune interferon act singly and in combination to reorganize human vascular endothelial cell monolayers.
Stolpen AH, Guinan EC, Fiers W, Pober JS. Recombinant tumor necrosis factor and immune interferon act singly and in combination to reorganize human vascular endothelial cell monolayers. American Journal Of Pathology 1986, 123: 16-24. PMID: 2421578, PMCID: PMC1888144.Peer-Reviewed Original ResearchConceptsRecombinant tumor necrosis factorHuman endothelial cellsTumor necrosis factorNecrosis factorMorphologic changesRecombinant immune interferonSimilar morphologic changesUnique morphologic changesVascular endothelial cell monolayersVascular responsesEndothelial cell monolayersPrimary human endothelial cellsEndothelial cell morphologyVascular liningConfluent primary culturesEpithelioid organizationImmune interferonEndothelial cellsSpinelike processesPrimary culturesTwo distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells.
Pober JS, Bevilacqua MP, Mendrick DL, Lapierre LA, Fiers W, Gimbrone MA. Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells. The Journal Of Immunology 1986, 136: 1680-7. PMID: 3485132, DOI: 10.4049/jimmunol.136.5.1680.Peer-Reviewed Original ResearchINDUCIBLE EXPRESSION OF CLASS II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS AND THE IMMUNOGENICITY OF VASCULAR ENDOTHELIUM
POBER J, COLLINS T, GIMBRONE M, LIBBY P, REISS C. INDUCIBLE EXPRESSION OF CLASS II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS AND THE IMMUNOGENICITY OF VASCULAR ENDOTHELIUM. Transplantation 1986, 41: 141-146. PMID: 2935977, DOI: 10.1097/00007890-198602000-00001.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsRecombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro.
Collins T, Lapierre LA, Fiers W, Strominger JL, Pober JS. Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 446-450. PMID: 3455781, PMCID: PMC322876, DOI: 10.1073/pnas.83.2.446.Peer-Reviewed Original ResearchConceptsClass II MHC antigensTumor necrosis factorClass I major histocompatibility complexI major histocompatibility complexRecombinant human tumor necrosis factorMajor histocompatibility complexHuman tumor necrosis factorHuman endothelial cellsMHC antigensSurface expressionNecrosis factorMRNA levelsHuman dermal fibroblastsEndothelial cellsClass IPresence of TNFCultured human endothelial cellsType I interferonVascular endothelial cellsMHC gene expressionMHC gene regulationDermal fibroblastsImmunoregulatory moleculesB antigensI interferon
1985
Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion.
Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA. Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion. American Journal Of Pathology 1985, 121: 394-403. PMID: 3878084, PMCID: PMC1887931.Peer-Reviewed Original ResearchConceptsInterleukin-1Procoagulant activityHuman endothelial cellsEndothelial cellsVascular endotheliumTissue factor-like procoagulant activityLeukocyte adhesionPeripheral blood polymorphonuclear leukocytesHuman peripheral blood polymorphonuclear leukocytesCultured human vascular endothelial cellsBlood polymorphonuclear leukocytesIL-1 actionIL-1 effectsRecombinant IL-1Sites of inflammationHuman vascular endothelial cellsInterleukin-1 activationVascular endothelial cellsEnhanced leukocyteIntravascular coagulationImmune mediatorsIL-2Saphenous veinEndothelial cell surfaceUmbilical veinInterleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. Journal Of Clinical Investigation 1985, 76: 2003-2011. PMID: 3877078, PMCID: PMC424265, DOI: 10.1172/jci112200.Peer-Reviewed Original ResearchConceptsEndothelial-leukocyte adhesionHuman vascular endotheliumIL-1Leukocyte adhesionVascular endotheliumCultured human vascular endotheliumLeukocyte-vessel wall interactionsIL-1 pretreatmentEndothelial cell damageEndothelial monolayersSites of inflammationIL-1 treatmentHuman umbilical vein endothelial monolayersCertain vascular diseasesPromyelocytic cell line HL-60Polymorphonuclear leukocyte adhesionIL-1 inductionImmune mediatorsHuman endothelial cellsEndothelial adhesivityVascular diseaseBlood leukocytesInterleukin-1Leukocyte cell linesCell line U937