2023
Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Huang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.Peer-Reviewed Original ResearchConceptsBiofilm matrix exopolysaccharideFacilitate host colonizationVibrio cholerae biofilmsΒ-propeller domainMatrix exopolysaccharideModular domainsHost colonizationRedundant rolesDistinct functionsAbiotic surfacesAdhesive proteinsHost surfaceHuman pathogensVibrio choleraeAdhesinsBacterial biofilmsHost tissuesColonization modelColonizationBAP1BiofilmsPathogensAntibiotic resistanceRBMCDomainNew Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology
Tai J, Ferrell M, Yan J, Waters C. New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology. Advances In Experimental Medicine And Biology 2023, 1404: 17-39. PMID: 36792869, PMCID: PMC10726288, DOI: 10.1007/978-3-031-22997-8_2.Peer-Reviewed Original ResearchConceptsV. cholerae biofilmsBacterial signal transduction networkSignal transduction networksBiofilm formationVibrio cholerae biofilmsKey model systemV. choleraeEcological roleMicrobial ecologyTransduction networksBiofilm maturationEnvironmental survivalCholera pathogenesisMolecular biophysicsRegulatory systemVibrio choleraeEl Tor biotypeBacterial surface attachmentRecent insightsModel systemBiofilmsLife cycleSurface attachmentCholeraeCentral function
2022
Molecular Mechanism of Vibrio cholerae biofilm adhesion
Weerasekera R, Hinbest A, Nero T, Huang X, Yan J, Olson R. Molecular Mechanism of Vibrio cholerae biofilm adhesion. The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.l7499.Peer-Reviewed Original ResearchΒ-prism domainV. cholerae biofilmsBiofilm matrixAbiotic surfacesCommunity of microorganismsComplex N-glycansΒ-propeller domainHost colonizationSequence identityAdhesion proteinsDistinct functionsMolecular mechanismsN-glycansBacterial cellsFunctional assaysHost surfaceVirulence factorsVibrio choleraeProteinMain proteinsV. choleraeSpecific adhesion moleculesBiofilmsBAP1Cholerae
2017
Extracellular-matrix-mediated osmotic pressure drives Vibrio cholerae biofilm expansion and cheater exclusion
Yan J, Nadell CD, Stone HA, Wingreen NS, Bassler BL. Extracellular-matrix-mediated osmotic pressure drives Vibrio cholerae biofilm expansion and cheater exclusion. Nature Communications 2017, 8: 327. PMID: 28835649, PMCID: PMC5569112, DOI: 10.1038/s41467-017-00401-1.Peer-Reviewed Original ResearchConceptsBiofilm-forming bacterial speciesSurface-attached communitiesVibrio choleraeActive cell growthModel organismsMatrix-producing cellsBacterial lifeNutrient uptakeEnvironmental perturbationsBacterial speciesBacterial cellsPlanktonic cellsSubmerged biofilmsExtracellular matrixCell growthMatrix productionOsmotic pressureBiofilm growthBiofilmsCholeraeCellsPhysical exclusionMatrix crosslinkingOsmotic swellingGrowth