Jing Yan, PhD
Assistant Professor, Molecular, Cellular and Developmental BiologyCards
About
Research
Publications
Featured Publications
Bacteria surfing the elastic wave
Nijjer J, Cohen T, Yan J. Bacteria surfing the elastic wave. Nature Physics 2022, 19: 6-7. DOI: 10.1038/s41567-022-01862-y.Peer-Reviewed Original ResearchSocial evolution of shared biofilm matrix components
Tai J, Mukherjee S, Nero T, Olson R, Tithof J, Nadell C, Yan J. Social evolution of shared biofilm matrix components. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2123469119. PMID: 35771939, PMCID: PMC9271185, DOI: 10.1073/pnas.2123469119.Peer-Reviewed Original ResearchConceptsBiofilm matrix componentsCooperative public goodsMatrix componentsBiofilm formationBiofilm matrix proteinsMatrix productionSocial evolution theoryBiofilm spatial structureCell surface adhesionEvolutionary timescalesCell clustersRelatedness coefficientsBacterial communitiesBiofilm habitatAdhesion proteinsEvolutionary advantageEvolutionary stabilityMatrix proteinsAssociated analysis toolsDiffusible compoundsHost environmentSocial evolutionUbiquitous modeOutstanding questionsProteinMechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix
Zhang Q, Nguyen D, Tai J, Xu X, Nijjer J, Huang X, Li Y, Yan J. Mechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix. Advanced Functional Materials 2022, 32 DOI: 10.1002/adfm.202110699.Peer-Reviewed Original ResearchMechanical resilienceNonlinear viscoelastic behaviorMaterials science pointLarge shear forcesBiofilm removal strategiesMechanical behaviorViscoelastic materialsViscoelastic behaviorBiofilm mechanicsSoft materialsStructure-property relationshipsRheological regimesRheological measurementsShear forceFunctional soft materialsLarge mechanical perturbationsPhysical insightPolymeric networkRemoval strategiesDynamics simulationsMolecular dynamics simulationsMaterialsDynamic environmentMatrixMechanical perturbationsMechanical forces drive a reorientation cascade leading to biofilm self-patterning
Nijjer J, Li C, Zhang Q, Lu H, Zhang S, Yan J. Mechanical forces drive a reorientation cascade leading to biofilm self-patterning. Nature Communications 2021, 12: 6632. PMID: 34789754, PMCID: PMC8599862, DOI: 10.1038/s41467-021-26869-6.Peer-Reviewed Original ResearchConceptsVibrio cholerae biofilmsSurface-attached aggregatesBacterial communitiesCell-surface interactionsDevelopmental processesBiofilm developmentBacterial cellsCell reorientationExtracellular matrixNonadherent mutantsDifferential growthBacterial biofilmsMechanical forcesBiofilmsBacterial growthDifferential orderingCellsGrowthMutantsLarge collectionMorphogenesis and cell ordering in confined bacterial biofilms
Zhang Q, Li J, Nijjer J, Lu H, Kothari M, Alert R, Cohen T, Yan J. Morphogenesis and cell ordering in confined bacterial biofilms. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2107107118. PMID: 34330824, PMCID: PMC8346881, DOI: 10.1073/pnas.2107107118.Peer-Reviewed Original ResearchConceptsInherent developmental programsBiofilm-dwelling cellsBiofilm-forming speciesSingle-cell imagingDevelopmental programSpecific matrix componentsCell orderingBiofilm developmentSpherical biofilmsBacterial cellsCell communityExtracellular matrixBiofilm morphologyBacterial biofilmsBiophysical mechanismsMatrix componentsHost tissuesBiofilmsBiofilm growthStiff gelsCellsMorphogenesisMutagenesisSpeciesVibrio
2024
Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS).
Weerasekera R, Moreau A, Huang X, Nam K, Hinbest A, Huynh Y, Liu X, Ashwood C, Pepi L, Paulson E, Cegelski L, Yan J, Olson R. Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS). PLOS Pathogens 2024, 20: e1012750. PMID: 39621768, DOI: 10.1371/journal.ppat.1012750.Peer-Reviewed Original ResearchVibrio polysaccharideBiofilm dispersalHuman pathogen Vibrio choleraeV. cholerae biofilmsPathogen Vibrio choleraeBiofilm disruption activityFormation of biofilmsVibrio coralliilyticusPolysaccharide lyasesLyase mechanismV. choleraeVibrio choleraeBiofilm matrixPathogenic bacteriaRbmBAntibacterial enzymesSecreted macromoleculesSolid-state NMRGlycolytic enzymesFluorescence-based biochemical assayBiofilmBiochemical assaysEnzymeSecreted glycosidasesDouble bondAgent-based modeling of stress anisotropy driven nematic ordering in growing biofilms
Li C, Nijjer J, Feng L, Zhang Q, Yan J, Zhang S. Agent-based modeling of stress anisotropy driven nematic ordering in growing biofilms. Soft Matter 2024, 20: 3401-3410. PMID: 38563244, PMCID: PMC11041162, DOI: 10.1039/d3sm01535a.Peer-Reviewed Original ResearchConceptsStress anisotropyShear stressShear stress relaxationHigh shear stressLow shear stressHydrostatic stressStress relaxationBiomedical applicationsTime delayComplex multicellular patternsMicroscopy imagesSpatial organization of cellsThree-dimensionalCell orderingNematic orderOrganization of cellsMulticellular patternsSpatiotemporal correlationAgarose gelBacterial biofilmsStressBiofilmStress landscapeGrowth conditionsAnisotropyLipoarabinomannan mediates localized cell wall integrity during division in mycobacteria
Sparks I, Kado T, Prithviraj M, Nijjer J, Yan J, Morita Y. Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria. Nature Communications 2024, 15: 2191. PMID: 38467648, PMCID: PMC10928101, DOI: 10.1038/s41467-024-46565-5.Peer-Reviewed Original ResearchConceptsCell wall integrityWall integrityRod cell shapeCell envelope integrityHost-pathogen interactionsCell envelope componentsClinically relevant pathogensAssociated with divisionBiosynthetic mutantsEnvelope integritySubcellular locationMycobacterium smegmatisOld poleMulti-septateCell shapeMutantsBacterial modelRelevant pathogensSeptal placementPhysiological functionsMycobacterium tuberculosisEnvelope componentsMycobacteriaLipoarabinomannanDiderm
2023
Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Huang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.Peer-Reviewed Original ResearchConceptsBiofilm matrix exopolysaccharideFacilitate host colonizationVibrio cholerae biofilmsΒ-propeller domainMatrix exopolysaccharideModular domainsHost colonizationRedundant rolesDistinct functionsAbiotic surfacesAdhesive proteinsHost surfaceHuman pathogensVibrio choleraeAdhesinsBacterial biofilmsHost tissuesColonization modelColonizationBAP1BiofilmsPathogensAntibiotic resistanceRBMCDomainNew Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology
Tai J, Ferrell M, Yan J, Waters C. New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology. Advances In Experimental Medicine And Biology 2023, 1404: 17-39. PMID: 36792869, PMCID: PMC10726288, DOI: 10.1007/978-3-031-22997-8_2.Peer-Reviewed Original ResearchConceptsV. cholerae biofilmsBacterial signal transduction networkSignal transduction networksBiofilm formationVibrio cholerae biofilmsKey model systemV. choleraeEcological roleMicrobial ecologyTransduction networksBiofilm maturationEnvironmental survivalCholera pathogenesisMolecular biophysicsRegulatory systemVibrio choleraeEl Tor biotypeBacterial surface attachmentRecent insightsModel systemBiofilmsLife cycleSurface attachmentCholeraeCentral function
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- Reconstructed 3D image of a Vibrio cholerae biofilm cluster. Color corresponds to height.