2012
Using a Fluorescent Cytosine Analogue tCo To Probe the Effect of the Y567 to Ala Substitution on the Preinsertion Steps of dNMP Incorporation by RB69 DNA Polymerase
Xia S, Beckman J, Wang J, Konigsberg WH. Using a Fluorescent Cytosine Analogue tCo To Probe the Effect of the Y567 to Ala Substitution on the Preinsertion Steps of dNMP Incorporation by RB69 DNA Polymerase. Biochemistry 2012, 51: 4609-4617. PMID: 22616982, PMCID: PMC3437246, DOI: 10.1021/bi300241m.Peer-Reviewed Original ResearchProbing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA
Xia S, Christian TD, Wang J, Konigsberg WH. Probing Minor Groove Hydrogen Bonding Interactions between RB69 DNA Polymerase and DNA. Biochemistry 2012, 51: 4343-4353. PMID: 22571765, PMCID: PMC3374494, DOI: 10.1021/bi300416z.Peer-Reviewed Original Research
2010
Substitution of Ala for Tyr567 in RB69 DNA Polymerase Allows dAMP and dGMP To Be Inserted opposite Guanidinohydantoin,
Beckman J, Wang M, Blaha G, Wang J, Konigsberg WH. Substitution of Ala for Tyr567 in RB69 DNA Polymerase Allows dAMP and dGMP To Be Inserted opposite Guanidinohydantoin,. Biochemistry 2010, 49: 8554-8563. PMID: 20795733, PMCID: PMC3755731, DOI: 10.1021/bi100913v.Peer-Reviewed Original Research
2009
RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity
Zhang H, Beckman J, Wang J, Konigsberg W. RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity. Biochemistry 2009, 48: 6940-6950. PMID: 19522539, PMCID: PMC2847438, DOI: 10.1021/bi900422b.Peer-Reviewed Original ResearchMeSH KeywordsBacteriophagesBase PairingDNA-Directed DNA PolymeraseKineticsModels, MolecularMutationSpectrometry, FluorescenceConceptsBase pairsCorrect base pairReplicative DNA polymerasesRB69 polRB69 DNA Polymerase MutantsNascent base pairDouble mutantSingle mutantsTriple mutantNumber of substitutionsWild typeMutantsBacteriophage RB69DNA polymerase mutantsPolymerase mutantsDNA polymeraseBinding pocketsNegative selectionDNA polRapid incorporationCatalytic centerLow incorporation efficiencyG mutationSulfolobus solfataricus Dpo4Base discrimination
1997
Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69
Wang J, Sattar A, Wang C, Karam J, Konigsberg W, Steitz T. Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69. Cell 1997, 89: 1087-1099. PMID: 9215631, DOI: 10.1016/s0092-8674(00)80296-2.Peer-Reviewed Original ResearchBacteriophagesBinding SitesConserved SequenceCrystallographyDNA Polymerase IDNA Polymerase IIDNA, Single-StrandedEscherichia coliExonucleasesGene Expression Regulation, ViralHIVProtein ConformationProtein Structure, SecondaryProtein Structure, TertiaryReplication OriginRNA-Directed DNA PolymeraseSequence Homology, Amino Acid