2023
Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior
Benisty H, Barson D, Moberly A, Lohani S, Tang L, Coifman R, Crair M, Mishne G, Cardin J, Higley M. Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior. Nature Neuroscience 2023, 27: 148-158. PMID: 38036743, PMCID: PMC11316935, DOI: 10.1038/s41593-023-01498-y.Peer-Reviewed Original ResearchConceptsFunctional connectivitySpontaneous behaviorCortical networksCortical network activityTime-varying functional connectivityFunctional magnetic resonanceCerebral cortexAwake miceDynamic functional connectivityAwake animalsNeighboring neuronsPatterned activityDistinct behavioral statesTwo-photon microscopyNeural activityCortical signalsBehavioral statesCortexNetwork activityCortical dynamicsMagnetic resonancePutting the brakes on synchrony: VIP interneurons tune visually evoked rhythmic activity
Perrenoud Q, Cardin J. Putting the brakes on synchrony: VIP interneurons tune visually evoked rhythmic activity. Neuron 2023, 111: 297-299. PMID: 36731427, DOI: 10.1016/j.neuron.2023.01.004.Peer-Reviewed Original Research
2010
Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
Cardin JA, Carlén M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nature Protocols 2010, 5: 247-254. PMID: 20134425, PMCID: PMC3655719, DOI: 10.1038/nprot.2009.228.Peer-Reviewed Original ResearchConceptsOptical interferenceMajor long-term goalViral vectorsCell-type selectivityRecording of neuronsOptogenetic stimulationChannelrhodopsin-2Cre-dependent expressionBrain circuit functionSelective cell typesInhibitory interneuronsIntracellular recordingsVivo electrophysiologyExcitatory neuronsIntact brainType selectivityNeural subtypesOptogenetic techniquesSpecific populationsNeural activityCircuit functionNeuronsInterferenceCell typesStimulation
2009
Pinacidil induces vascular dilation and hyperemia in vivo and does not impact biophysical properties of neurons and astrocytes in vitro
Cao R, Higashikubo B, Cardin J, Knoblich U, Ramos R, Nelson M, Moore C, Brumberg J. Pinacidil induces vascular dilation and hyperemia in vivo and does not impact biophysical properties of neurons and astrocytes in vitro. Cleveland Clinic Journal Of Medicine 2009, 76: s80-s85. PMID: 19380306, PMCID: PMC4406396, DOI: 10.3949/ccjm.76.s2.16.Peer-Reviewed Original ResearchConceptsBlood volumeApplication of pinacidilWhole-cell recordingsLocal blood volumeFunctional hyperemiaVascular dilationNeocortical slicesAnesthetized ratsAwake miceSmooth muscleSelective agonistPinacidilImpact of hemodynamicsIntrinsic modulatorHyperemiaNeural activityVasodilationNeuronsVivoBiophysical propertiesNeural systemsHemodynamicsAgonistsNeocortexRats