2024
DeepVID v2: self-supervised denoising with decoupled spatiotemporal enhancement for low-photon voltage imaging
Liu C, Lu J, Wu Y, Ye X, Ahrens A, Platisa J, Pieribone V, Chen J, Tian L. DeepVID v2: self-supervised denoising with decoupled spatiotemporal enhancement for low-photon voltage imaging. Neurophotonics 2024, 11: 045007. PMID: 39474199, PMCID: PMC11519979, DOI: 10.1117/1.nph.11.4.045007.Peer-Reviewed Original ResearchDeep learning denoising methodsLeverage temporal informationSelf-supervised denoisingSuperior denoising capabilitiesReal-time inferenceLocal spatial informationSequence of framesVoltage imaging dataDenoising frameworkDenoising methodSignal-to-noise ratioDenoising capabilityExtraction branchDenoising needsBlind pixelsTemporal informationDenoisingGround truthPoisson noiseTemporal performanceCentral frameOffline versionSpatial informationDynamics of neuronal activitySignal-to-noisePlateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging
Milicevic K, Ivanova V, Lovic D, Platisa J, Andjus P, Antic S. Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging. Scientific Reports 2024, 14: 22787. PMID: 39367010, PMCID: PMC11452489, DOI: 10.1038/s41598-024-70319-4.Peer-Reviewed Original ResearchConceptsCa2+ transientsAction potentialsSpontaneously active neuronsCalcium imaging studiesCa2+ recordingsNeuronal action potentialsDepolarizing envelopeOptical signalsDendritic Ca2AP firingPlateau depolarizationsPyramidal neuronsMembrane depolarizationPlateau potentialsImaging studiesActive neuronsNeuronal culturesPhoton signaturesImaging modalitiesUP statesIn vivo conditionsNeuronsHalf-widthCa2+Depolarization
2023
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations
Platisa J, Ye X, Ahrens A, Liu C, Chen I, Davison I, Tian L, Pieribone V, Chen J. High-speed low-light in vivo two-photon voltage imaging of large neuronal populations. Nature Methods 2023, 20: 1095-1103. PMID: 36973547, PMCID: PMC10894646, DOI: 10.1038/s41592-023-01820-3.Peer-Reviewed Original ResearchConceptsNeuronal populationsLarge neuronal populationsNeural circuit functionTwo-photon voltage imagingAwake behaving miceCalcium imagingBehaving miceVoltage imagingCircuit functionNeuronsShot noise levelShot-noise limitImagingTwo-photon microscopeKilohertz frame ratesDeep-tissue imagingPopulationVoltage indicatorsField of viewMiceShot noiseTwo-photon voltage imaging denoising by self-supervised learning
Liu C, Platisa J, Ye X, Ahrens A, Chen I, Davison I, Pieribone V, Chen J, Tian L. Two-photon voltage imaging denoising by self-supervised learning. Progress In Biomedical Optics And Imaging 2023, 12365: 1236505-1236505-2. DOI: 10.1117/12.2648122.Peer-Reviewed Original Research
2022
Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight
Platisa J, Zeng H, Madisen L, Cohen LB, Pieribone VA, Storace DA. Voltage imaging in the olfactory bulb using transgenic mouse lines expressing the genetically encoded voltage indicator ArcLight. Scientific Reports 2022, 12: 1875. PMID: 35115567, PMCID: PMC8813909, DOI: 10.1038/s41598-021-04482-3.Peer-Reviewed Original ResearchConceptsTransgenic mouse lineMouse linesOlfactory bulbSubpopulation of interneuronsVivo mammalian brainTransgenic reporter animalsTransgenic reporter miceOlfactory receptor neuronsNeuronal electrical activityVoltage indicator ArcLightGlomerular layerReporter miceMammalian brainReceptor neuronsReporter animalsHigh expression levelsElectrical activityMembrane potential changesOdorant responsesNeural activityCell populationsSingle trialExpression levelsVivo experimentsDifferent cell typesDeepVID: A Self-supervised Deep Learning Framework for Two-photon Voltage Imaging Denoising
Liu C, Platisa J, Ye X, Ahrens A, Chen I, Davison I, Pieribone V, Chen J, Tian L. DeepVID: A Self-supervised Deep Learning Framework for Two-photon Voltage Imaging Denoising. 2022, btu4c.4. DOI: 10.1364/brain.2022.btu4c.4.Peer-Reviewed Original Research
2018
Genetically encoded fluorescent voltage indicators: are we there yet?
Platisa J, Pieribone VA. Genetically encoded fluorescent voltage indicators: are we there yet? Current Opinion In Neurobiology 2018, 50: 146-153. PMID: 29501950, PMCID: PMC5984684, DOI: 10.1016/j.conb.2018.02.006.Peer-Reviewed Original Research
2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2015
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment
Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLOS ONE 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.Peer-Reviewed Original ResearchConceptsAcid-binding proteinFluorescent proteinFatty acid-binding proteinDuplication eventsGene duplication eventsStrong positive selectionNew fluorescent proteinsMarine environmentAdaptive evolutionEvolutionary switchCryptic genusVertebrate ordersVertebrate taxaPhylogenetic reconstructionProtein familyPhylogenetic analysisSequence motifsSister groupEvolutionary adaptationTranscriptomic analysisPositive selectionFatty acid binding proteinAcid binding proteinBinding proteinBright green fluorescence
2014
Development of a Red Genetically-Encoded Voltage Indicator and its use with Channelrhodopsin for All-Optical Electrophysiology
Abdelfattah A, Platisa J, Zhao Y, Pieribone V, Campbell R. Development of a Red Genetically-Encoded Voltage Indicator and its use with Channelrhodopsin for All-Optical Electrophysiology. Biophysical Journal 2014, 106: 629a-630a. DOI: 10.1016/j.bpj.2013.11.3482.Peer-Reviewed Original Research
2013
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLOS ONE 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.Peer-Reviewed Original ResearchGenetically Targeted Optical Electrophysiology in Intact Neural Circuits
Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically Targeted Optical Electrophysiology in Intact Neural Circuits. Cell 2013, 154: 904-913. PMID: 23932121, PMCID: PMC3874294, DOI: 10.1016/j.cell.2013.07.027.Peer-Reviewed Original ResearchConceptsIntact neural circuitsNeural circuitsIntact brain tissueMembrane potentialNeuronal information processingNervous systemAction potentialsBrain tissueNeuronsStudy of intracellularElectrical activityKey cellular parametersMultiple neuronsElectrical eventsSubthreshold eventsNeurite branchesOptical electrophysiologyReliable recordingCellular parametersVoltage indicatorsFluorescent voltage indicatorsBrainIn Vivo Imaging of Odor-Evoked Responses in the Olfactory Bulb using Arclight, a Novel Fp Voltage Probe
Storace D, Sung U, Platisa J, Cohen L, Pieribone V. In Vivo Imaging of Odor-Evoked Responses in the Olfactory Bulb using Arclight, a Novel Fp Voltage Probe. Biophysical Journal 2013, 104: 679a. DOI: 10.1016/j.bpj.2012.11.3751.Peer-Reviewed Original Research
2012
Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals
Osman A, Park JH, Dickensheets D, Platisa J, Culurciello E, Pieribone VA. Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals. IEEE Transactions On Biomedical Circuits And Systems 2012, 6: 446-453. PMID: 23853231, DOI: 10.1109/tbcas.2012.2226174.Peer-Reviewed Original ResearchHerbicide Phosphinothricin Causes Direct Stimulation Hormesis
Dragićević M, Platiša J, Nikolić R, Todorović S, Bogdanović M, Mitić N, Simonović A. Herbicide Phosphinothricin Causes Direct Stimulation Hormesis. Dose-Response 2012, 11: dose-response.12-039.simonovic. PMID: 23983663, PMCID: PMC3748847, DOI: 10.2203/dose-response.12-039.simonovic.Peer-Reviewed Original ResearchPhosphinothricin concentrationGlutamine synthetaseCell-free systemInhibit glutamine synthetaseCytosolic GS1Nitrogen assimilationGS inhibitorsPlant deathBiomass productionGlutamine depletionMethionine sulfoximineMolecular mechanismsGrowth suppressionGrowth responseConcentration-dependent interactionGrowth inhibitionGrowth stimulationHerbicide phosphinothricinPhosphinothricinGS2Ammonia accumulationHoloenzymeGrowthSynthetaseInhibitionA Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original ResearchSingle Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron 2012, 75: 779-785. PMID: 22958819, PMCID: PMC3439164, DOI: 10.1016/j.neuron.2012.06.040.Peer-Reviewed Original ResearchA second-generation imaging system for freely moving animals
Park J, Platisa J, Pieribone V, Culurciello E. A second-generation imaging system for freely moving animals. 2005 IEEE International Symposium On Circuits And Systems (ISCAS) 2012, 105-108. DOI: 10.1109/iscas.2012.6271408.Peer-Reviewed Original ResearchGenetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics
Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, Platisa J, Pieribone V. Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. Journal Of Neuroscience Methods 2012, 208: 190-196. PMID: 22634212, PMCID: PMC3398169, DOI: 10.1016/j.jneumeth.2012.05.016.Peer-Reviewed Original Research