2000
Membrane Estrogen Receptor Engagement Activates Endothelial Nitric Oxide Synthase via the PI3-Kinase–Akt Pathway in Human Endothelial Cells
Haynes M, Sinha D, Russell K, Collinge M, Fulton D, Morales-Ruiz M, Sessa W, Bender J. Membrane Estrogen Receptor Engagement Activates Endothelial Nitric Oxide Synthase via the PI3-Kinase–Akt Pathway in Human Endothelial Cells. Circulation Research 2000, 87: 677-682. PMID: 11029403, DOI: 10.1161/01.res.87.8.677.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeBinding SitesCell MembraneCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsEstradiolGenes, DominantHumansMorpholinesNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktReceptors, EstrogenSerum Albumin, BovineSignal TransductionTransduction, GeneticConceptsPI3-kinaseKinase-Akt pathwayDominant-negative AktPI3-kinase inhibitorRapid eNOS phosphorylationRapid Akt phosphorylationActivation of eNOSAkt-dependent pathwayEndothelial nitric oxide synthaseAkt substratePhosphatidylinositol 3ENOS phosphorylationCritical residuesSerine 473Human endothelial cellsEstrogen receptor antagonist ICI 182Cell membrane sitesHuman endothelial cell lineAkt pathwayAkt phosphorylationPhosphorylationReceptor engagementEndothelial cell lineActivation eventsFunctional involvement
1998
Retroviral Gene Transfer: Effects on Endothelial Cell Phenotype
Inaba M, Toninelli E, Vanmeter G, Bender J, Conte M. Retroviral Gene Transfer: Effects on Endothelial Cell Phenotype. Journal Of Surgical Research 1998, 78: 31-36. PMID: 9733614, DOI: 10.1006/jsre.1998.5379.Peer-Reviewed Original ResearchConceptsEndothelial cellsTransduced ECsCell adhesion molecule profilesCell phenotypeHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsAdhesion molecule profileUpregulation of expressionGT protocolVein endothelial cellsTarget cell phenotypeViral exposureMHC-IICardiovascular diseaseActivation moleculeICAM-1Endothelial cell phenotypeVCAM-1E-selectinGene transferVivo gene transferCell countSystemic deliveryCytokine stimulationUnaltered pattern