Melanoma × Macrophage Fusion Hybrids Acquire Increased Melanogenesis and Metastatic Potential: Altered N‐Glycosylation as an Underlying Mechanism
SODI S, CHAKRABORTY A, PLATT J, KOLESNIKOVA N, ROSEMBLAT S, KEH‐YEN A, BOLOGNIA J, RACHKOVSKY M, ORLOW S, PAWELEK J. Melanoma × Macrophage Fusion Hybrids Acquire Increased Melanogenesis and Metastatic Potential: Altered N‐Glycosylation as an Underlying Mechanism. Pigment Cell & Melanoma Research 1998, 11: 299-309. PMID: 9877101, DOI: 10.1111/j.1600-0749.1998.tb00739.x.Peer-Reviewed Original ResearchConceptsN-glycosylationLAMP-1Parental cellsN-glycosylation inhibitorMelanoma cellsCloudman S91 cellsMetastatic potentialImportant regulatory pathwayAltered N-glycosylationMajority of hybridsParental melanoma cellsCloudman S91 melanoma cellsRegulatory pathwaysMelanosomal proteinsCloudman melanoma cellsMelanotic phenotypeSame proteinS91 melanoma cellsIncreased glycosylationPigmentary systemCell lysatesS91 cellsUnderlying mechanismCloudman cellsGel electrophoresisMelanoma × macrophage hybrids with enhanced metastatic potential
Rachkovsky M, Sodi S, Chakraborty A, Avissar Y, Bolognia J, Madison McNiff J, Platt J, Bermudes D, Pawelek J. Melanoma × macrophage hybrids with enhanced metastatic potential. Clinical & Experimental Metastasis 1998, 16: 299-312. PMID: 9626809, DOI: 10.1023/a:1006557228604.Peer-Reviewed Original ResearchConceptsHigh metastatic potentialMetastatic potentialMelanoma cellsTumor-infiltrating leukocytesParental melanoma cellsLow metastatic potentialB16F10 melanoma cellsFibroblast-conditioned mediumCloudman S91 mouse melanoma cellsMetastatic melanoma cellsS91 mouse melanoma cellsVascular invasionMetastatic melanomaPrimary tumorNormal miceMelanoma metastasesEnhanced metastasisMetastasisDistant organsUseful new modelMouse melanoma cellsHigher chemotaxisMetastatic linesHuman macrophagesTumor cells