2013
Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors
Park RJ, Shen H, Liu L, Liu X, Ferguson SM, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. Journal Of Cell Science 2013, 126: 5305-5312. PMID: 24046449, PMCID: PMC3828596, DOI: 10.1242/jcs.138578.Peer-Reviewed Original ResearchConceptsDynamin 1Dynamin inhibitorTKO cellsPeripheral membrane rufflingDouble knockoutDynamin 3 geneClathrin-mediated endocytosisTriple-knockout cellsDynamin-dependent processFluid-phase endocytosisDyngo-4aMembrane fissionMembrane rufflingDKO cellsDynamin 2Knockout cellsLow-level expressionCell physiologyDynaminEndocytosisTarget effectsDKO fibroblastsGenesCellsKO fibroblastsA Multiple Hypothesis Based Method for Particle Tracking and Its Extension for Cell Segmentation
Liang L, Shen H, Rompolas P, Greco V, De Camilli P, Duncan JS. A Multiple Hypothesis Based Method for Particle Tracking and Its Extension for Cell Segmentation. Lecture Notes In Computer Science 2013, 23: 98-109. PMID: 24683961, PMCID: PMC4122512, DOI: 10.1007/978-3-642-38868-2_9.Peer-Reviewed Original Research
2012
Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane
Sousa LP, Lax I, Shen H, Ferguson SM, De Camilli P, Schlessinger J. Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 4419-4424. PMID: 22371560, PMCID: PMC3311323, DOI: 10.1073/pnas.1200164109.Peer-Reviewed Original ResearchConceptsEGFR endocytosisPlasma membraneDynamin-mediated endocytosisEndocytosis of EGFRRole of endocytosisLow-affinity EGFRsEGF receptor activationCell surface receptorsMAPK responseMAPK stimulationEGFR degradationAkt stimulationCell signalingDynaminEGFR activationEGFR autophosphorylationAkt activationEndocytosisCell surfaceSurface receptorsMouse fibroblastsAkt responseReceptor activationHigh EGF concentrationsActivation
2010
Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin
Shen H, Ferguson SM, Dephoure N, Park R, Yang Y, Volpicelli-Daley L, Gygi S, Schlessinger J, De Camilli P. Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin. Molecular Biology Of The Cell 2010, 22: 493-502. PMID: 21169560, PMCID: PMC3038647, DOI: 10.1091/mbc.e10-07-0637.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCdc42 GTP-Binding ProteinCell LineChlorocebus aethiopsClathrinClathrin Heavy ChainsClathrin Light ChainsCoated Pits, Cell-MembraneCOS CellsDynamin IDynamin IIEndocytosisFibroblastsGene Knockout TechniquesHumansMiceMice, KnockoutPhosphorylationProtein BindingProtein-Tyrosine KinasesSignal TransductionConceptsEndocytic clathrin-coated pitsClathrin-coated pitsRNA interference-based approachesFundamental cellular processesGrowth factor receptor signalingWild-type cellsNonreceptor tyrosine kinaseActive Cdc42Cellular processesSpecific cargoClathrin assemblyEndocytic routeEndocytic vesiclesGrowth factor receptorTyrosine phosphorylationPhosphorylation stateClathrin boxTyrosine kinaseKinase phosphorylationCdc42Receptor signalingActivation-deactivation cyclePhosphorylationDynaminFactor receptor