2021
The Essential Function of SETDB1 in Homologous Chromosome Pairing and Synapsis during Meiosis
Cheng EC, Hsieh CL, Liu N, Wang J, Zhong M, Chen T, Li E, Lin H. The Essential Function of SETDB1 in Homologous Chromosome Pairing and Synapsis during Meiosis. Cell Reports 2021, 34: 108575. PMID: 33406415, PMCID: PMC8513770, DOI: 10.1016/j.celrep.2020.108575.Peer-Reviewed Original ResearchConceptsEarly meiosisEarly meiotic prophase IFunction of SETDB1Homologous chromosome pairingMeiotic prophase IHistone-lysine N-methyltransferaseMeiotic silencingSurvival of spermatocytesGermline developmentBouquet formationHomologous chromosomesLineage genesChromosome pairingBivalent formationPericentromeric regionProphase IApoptosis of spermatocytesSETDB1Essential functionsHomologous bivalentsH3K9me3Meiotic arrestMeiosisSpermatocytesN-methyltransferase
2020
MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA
Hsieh C, Xia J, Lin H. MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA. The EMBO Journal 2020, 39: embj2019103614. PMID: 32677148, PMCID: PMC7429737, DOI: 10.15252/embj.2019103614.Peer-Reviewed Original ResearchConceptsChromosome misalignmentSatellite RNAKinetochore assemblySatellite repeatsWild-type spermatocytesPericentromeric satellite repeatsFaithful chromosome segregationProper kinetochore assemblyChromosome mis-segregationPost-transcriptional regulationPiRNA biogenesisMeiotic functionsPIWI proteinsChromosome segregationMis-segregationMurine memberElevated aneuploidyMale meiosisPrevents aneuploidyDicer cleavageMIWIMetaphase IRNA fragmentsMeiosisRNA
2018
A role of Pumilio 1 in mammalian oocyte maturation and maternal phase of embryogenesis
Mak W, Xia J, Cheng EC, Lowther K, Lin H. A role of Pumilio 1 in mammalian oocyte maturation and maternal phase of embryogenesis. Cell & Bioscience 2018, 8: 54. PMID: 30364263, PMCID: PMC6194604, DOI: 10.1186/s13578-018-0251-1.Peer-Reviewed Original ResearchAbnormal preimplantation developmentPreimplantation developmentPumilio 1Genome-wide transcriptome analysisMaternal effect genesPost-transcriptional regulatorsMammalian oocyte maturationMaternal phaseCDK1 mRNAEmbryo transitionMammalian embryogenesisEffect genesTranscriptome analysisMaternal mRNAsMammalian systemsMRNA targetsPUM1CDK1 proteinConcomitant perturbationMeiotic maturationEmbryogenesisOocyte maturationMeiosisImportant functionsProtein
2011
Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse
Beyret E, Lin H. Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse. Developmental Biology 2011, 355: 215-226. PMID: 21539824, PMCID: PMC3443393, DOI: 10.1016/j.ydbio.2011.04.021.Peer-Reviewed Original ResearchConceptsPIWI proteinsXY bodyMouse testisAdult mouse testisEarly round spermatidsSex chromosomesGenomic sequencesPiRNAsChromatoid bodyCytological characterizationPachytene stageGerm cellsPhenotypic analysisAdult testisRound spermatidsProteinMeiosisSpermatogenesisCytoplasmDense bodiesSpermatocytesTestisPiRNAChromosomesTransposon