1998
[16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry
Rudnick G. [16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry. Methods In Enzymology 1998, 296: 233-247. PMID: 9779452, DOI: 10.1016/s0076-6879(98)96018-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNumber of ionsKinetic determinationMolecular machinesCharge movesIonsLipid bilayersNeurotransmitter moleculesMoleculesInput of energySteady-state distributionLow concentrationsChargeElectrical potentialMembraneIon gradientsStoichiometryHigh concentrationsUltimate concentrationNaConcentrationEnergyBilayersNeurotransmitter transportersNumber of NaNeurotransmitter transport
1993
Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding.
Wall S, Innis R, Rudnick G. Binding of the cocaine analog 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane to serotonin and dopamine transporters: different ionic requirements for substrate and 2 beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binding. Molecular Pharmacology 1993, 43: 264-70. PMID: 8429827.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveBlood PlateletsCarrier ProteinsCocaineCorpus StriatumDopamineDopamine Plasma Membrane Transport ProteinsHydrogen-Ion ConcentrationIn Vitro TechniquesIonsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsOsmolar ConcentrationProtein BindingRadioligand AssayRatsSerotoninSerotonin Plasma Membrane Transport Proteins
1992
p-Chloroamphetamine induces serotonin release through serotonin transporters.
Rudnick G, Wall S. p-Chloroamphetamine induces serotonin release through serotonin transporters. Biochemistry 1992, 31: 6710-8. PMID: 1322169, DOI: 10.1021/bi00144a010.Peer-Reviewed Original ResearchMeSH KeywordsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneChloridesHumansHydrogen-Ion ConcentrationImipramineKineticsLithiumLithium ChlorideMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsP-ChloroamphetamineRadioisotope Dilution TechniqueSerotoninSerotonin Plasma Membrane Transport ProteinsTritiumConceptsPlasma membrane vesiclesMembrane vesiclesChromaffin granule membrane vesiclesVesicular amine transporterATP hydrolysisBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesPrevents accumulationAmine transportersPresence of Mg2TransportersVesiclesChromaffin granulesModel systemDelta pHHuman plateletsATPEffluxManner characteristicAccumulationTransmembraneNaCl gradientPCA's abilityP-chloroamphetamineThe molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release.
Rudnick G, Wall S. The molecular mechanism of "ecstasy" [3,4-methylenedioxy-methamphetamine (MDMA)]: serotonin transporters are targets for MDMA-induced serotonin release. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 1817-1821. PMID: 1347426, PMCID: PMC48544, DOI: 10.1073/pnas.89.5.1817.Peer-Reviewed Original ResearchConceptsPlasma membrane vesiclesMembrane vesiclesAmine transportersVesicular amine transporterBiogenic amine transportersSecretory vesiclesPlasma membraneATP hydrolysisMolecular mechanismsBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesTransportersDirect interactionVesiclesChromaffin granulesHuman plateletsManner characteristicEffluxTransmembraneATPMDMA actionMechanismBindingMembrane
1991
Binding of the cocaine analog 2 beta-[3H] carboxymethoxy-3 beta-(4-fluorophenyl)tropane to the serotonin transporter.
Rudnick G, Wall S. Binding of the cocaine analog 2 beta-[3H] carboxymethoxy-3 beta-(4-fluorophenyl)tropane to the serotonin transporter. Molecular Pharmacology 1991, 40: 421-6. PMID: 1896028.Peer-Reviewed Original Research
1990
Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter.
Rudnick G, Steiner-Mordoch S, Fishkes H, Stern-Bach Y, Schuldiner S. Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter. Biochemistry 1990, 29: 603-8. PMID: 2140052, DOI: 10.1021/bi00455a002.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmmonium SulfateBinding SitesBiogenic AminesCarbonyl Cyanide m-Chlorophenyl HydrazoneCarrier ProteinsChloridesChromaffin GranulesChromaffin SystemDetergentsElectrochemistryHydrogen-Ion ConcentrationIonophoresKineticsLigandsMembrane PotentialsReserpineThermodynamicsThiocyanatesTime FactorsUreaConceptsBiogenic amine transportersReserpine bindingChromaffin granule membrane vesiclesAmine transportersAmine transportMembrane vesiclesDead-end complexIon translocationTransportersSubstrate moleculesBindingM ureaDelta pHTranslocationTriton XSodium dodecyl sulfateNeutral substrate moleculesTransmembraneHigh-affinity siteUDP-GlcNAc transport across the Golgi membrane: electroneutral exchange for dianionic UMP.
Waldman B, Rudnick G. UDP-GlcNAc transport across the Golgi membrane: electroneutral exchange for dianionic UMP. Biochemistry 1990, 29: 44-52. PMID: 2322548, DOI: 10.1021/bi00453a006.Peer-Reviewed Original Research
1985
Relative lack of ATP-driven H+ translocase activity in isolated parotid secretory granules.
Arvan P, Rudnick G, Castle J. Relative lack of ATP-driven H+ translocase activity in isolated parotid secretory granules. Journal Of Biological Chemistry 1985, 260: 14945-14952. PMID: 2866180, DOI: 10.1016/s0021-9258(18)95684-x.Peer-Reviewed Original ResearchAcetatesAcetic AcidAdenosine TriphosphateAnimalsCarbonyl Cyanide m-Chlorophenyl HydrazoneCarbonyl Cyanide p-TrifluoromethoxyphenylhydrazoneCell Membrane PermeabilityCytoplasmic GranulesEthylmaleimideHydrogen-Ion ConcentrationHydrolysisMembrane PotentialsMethylaminesParotid GlandProton-Translocating ATPasesRatsSodium CyanideEffects of monovalent cations on Semliki Forest virus entry into BHK-21 cells.
Helenius A, Kielian M, Wellsteed J, Mellman I, Rudnick G. Effects of monovalent cations on Semliki Forest virus entry into BHK-21 cells. Journal Of Biological Chemistry 1985, 260: 5691-5697. PMID: 3988769, DOI: 10.1016/s0021-9258(18)89078-0.Peer-Reviewed Original ResearchConceptsSemliki Forest virusEndosome membraneViral envelopeBaby hamster kidney cellsMammalian cellsEndosomal membranesLow endosomalBHK-21 cellsHamster kidney cellsVirus endocytosisIntact cellsConformational changesEndosomesPrelysosomal endosomesViral spike glycoproteinVirus fusionKidney cellsAcidic endosomal pHViral RNAVoltage-sensitive probeEndocytosisVirus entryEndosomal pHCellsSpike glycoprotein
1984
Osmotic properties and internal pH of isolated rat parotid secretory granules.
Arvan P, Rudnick G, Castle J. Osmotic properties and internal pH of isolated rat parotid secretory granules. Journal Of Biological Chemistry 1984, 259: 13567-13572. PMID: 6490664, DOI: 10.1016/s0021-9258(18)90730-1.Peer-Reviewed Original Research
1983
Hydrogen ion cotransport by the renal brush border glutamate transporter.
Nelson P, Dean G, Aronson P, Rudnick G. Hydrogen ion cotransport by the renal brush border glutamate transporter. Biochemistry 1983, 22: 5459-63. PMID: 6140027, DOI: 10.1021/bi00292a030.Peer-Reviewed Original ResearchAcidification of macrophage and fibroblast endocytic vesicles in vitro.
Galloway C, Dean G, Marsh M, Rudnick G, Mellman I. Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 3334-3338. PMID: 6190176, PMCID: PMC394037, DOI: 10.1073/pnas.80.11.3334.Peer-Reviewed Original ResearchConceptsAcidic prelysosomal compartmentEndosome-lysosome fusionATP-dependent acidificationF0-ATPaseEndocytic vesiclesPrelysosomal compartmentAddition of ATPPrelysosomal vacuolesMitochondrial F1LysosomesEndosomesSecretory granulesCultured macrophagesN-ethylmaleimideInternal pHPercoll density gradientsAcidificationPH gradientFibroblastsCompartmentsOrganellesInhibitorsPermeant anionsFluorescein-dextranVacuoles
1982
Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules.
Fishkes H, Rudnick G. Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules. Journal Of Biological Chemistry 1982, 257: 5671-5677. PMID: 7068613, DOI: 10.1016/s0021-9258(19)83830-9.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateBiological TransportBlood PlateletsCytoplasmic GranulesHumansHydrogen-Ion ConcentrationIntracellular MembranesKineticsL-Lactate DehydrogenaseSerotoninConceptsPlatelet dense granulesMembrane vesiclesSerotonin transport activitySoluble enzymatic activityPresence of ATPCarbonyl cyanide p-trifluoromethoxyphenylhydrazoneAddition of ATPOsmotic lysatesTransport activityCyanide p-trifluoromethoxyphenylhydrazoneEnzymatic activityDensity gradient centrifugationVesiclesSerotonin transportVesicle interiorDense granulesMembrane potentialP-trifluoromethoxyphenylhydrazoneGradient centrifugationATPAssay mediumAdenine nucleotidesBioenergeticsNucleotidesGranulesCoupling of transmembrane proton gradients to platelet serotonin transport.
Keyes S, Rudnick G. Coupling of transmembrane proton gradients to platelet serotonin transport. Journal Of Biological Chemistry 1982, 257: 1172-1176. PMID: 7056713, DOI: 10.1016/s0021-9258(19)68170-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBlood PlateletsCell MembraneHydrogen-Ion ConcentrationKineticsPotassiumSerotoninSwineConceptsPlasma membrane vesiclesSerotonin transportTransmembrane proton gradientPlatelet plasma membraneTransmembrane ion gradientsApparent competitionPlasma membraneMembrane vesiclesProton gradientIon gradientsSerotonin accumulationSole driving forceVesiclesAccumulationSerotonin transporterPH differencePhysiological pHTransportersTransportPH stimulationMembraneGradient
1980
Evidence for two distinct serotonin transport systems in platelets.
Rudnick G, Fishkes H, Nelson P, Schuldiner S. Evidence for two distinct serotonin transport systems in platelets. Journal Of Biological Chemistry 1980, 255: 3638-3641. PMID: 7364762, DOI: 10.1016/s0021-9258(19)85751-4.Peer-Reviewed Original Research