2010
Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*
Tavoulari S, Rizwan AN, Forrest LR, Rudnick G. Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*. Journal Of Biological Chemistry 2010, 286: 2834-2842. PMID: 21115480, PMCID: PMC3024779, DOI: 10.1074/jbc.m110.186064.Peer-Reviewed Original ResearchConceptsChloride-binding siteConformational changesAdjacent binding sitesSingle point mutationProkaryotic homologuesSubstrate translocationIon-binding sitesTransporter homologueTransport proteinsNeurotransmitter transportersNeurotransmitter transportPoint mutationsBinding sitesHomologuesProteinMutationsCl(-) bindsDirect evidenceTherapeutic drugsSitesDependent formTranslocationTransportersBindsResidues
1990
Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter.
Rudnick G, Steiner-Mordoch S, Fishkes H, Stern-Bach Y, Schuldiner S. Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter. Biochemistry 1990, 29: 603-8. PMID: 2140052, DOI: 10.1021/bi00455a002.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmmonium SulfateBinding SitesBiogenic AminesCarbonyl Cyanide m-Chlorophenyl HydrazoneCarrier ProteinsChloridesChromaffin GranulesChromaffin SystemDetergentsElectrochemistryHydrogen-Ion ConcentrationIonophoresKineticsLigandsMembrane PotentialsReserpineThermodynamicsThiocyanatesTime FactorsUreaConceptsBiogenic amine transportersReserpine bindingChromaffin granule membrane vesiclesAmine transportersAmine transportMembrane vesiclesDead-end complexIon translocationTransportersSubstrate moleculesBindingM ureaDelta pHTranslocationTriton XSodium dodecyl sulfateNeutral substrate moleculesTransmembraneHigh-affinity site
1989
Zwitterionic and Anionic Forms of a Serotonin Analog as Transport Substrates
Rudnick G, Kirk K, Fishkes H, Schuldiner S. Zwitterionic and Anionic Forms of a Serotonin Analog as Transport Substrates. Journal Of Biological Chemistry 1989, 264: 14865-14868. PMID: 2549040, DOI: 10.1016/s0021-9258(18)63781-0.Peer-Reviewed Original ResearchConceptsBiogenic amine transportersAmine transportersPlasma membrane serotonin transporterSubstrate translocationBovine chromaffin granulesTransport substratesHuman blood plateletsSerotonin transportChromaffin granulesTransportersBlood plateletsSerotonin transporterKmIdentical ratesTranslocationSubstrateTransport