1984
The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases.
Dean G, Fishkes H, Nelson P, Rudnick G. The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases. Journal Of Biological Chemistry 1984, 259: 9569-9574. PMID: 6204985, DOI: 10.1016/s0021-9258(17)42738-4.Peer-Reviewed Original ResearchConceptsGranule ATPaseGranule membranesMitochondrial membraneATP hydrolysisIon-translocating ATPasesEnergy-transducing membranesDense granule membraneIdentical assay conditionsF1F0-ATPasesSubstrate specificityPlasma membraneMitochondrial ATPaseInhibitor sensitivityATPasesATPaseATPase activityConcentrations of NaN3Cation requirementATPase moleculesMembraneN-ethylmaleimideAssay conditionsAdenosine triphosphataseEfrapeptinOligomycin
1983
Acidification of macrophage and fibroblast endocytic vesicles in vitro.
Galloway C, Dean G, Marsh M, Rudnick G, Mellman I. Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 3334-3338. PMID: 6190176, PMCID: PMC394037, DOI: 10.1073/pnas.80.11.3334.Peer-Reviewed Original ResearchConceptsAcidic prelysosomal compartmentEndosome-lysosome fusionATP-dependent acidificationF0-ATPaseEndocytic vesiclesPrelysosomal compartmentAddition of ATPPrelysosomal vacuolesMitochondrial F1LysosomesEndosomesSecretory granulesCultured macrophagesN-ethylmaleimideInternal pHPercoll density gradientsAcidificationPH gradientFibroblastsCompartmentsOrganellesInhibitorsPermeant anionsFluorescein-dextranVacuoles