1998
Critical Amino Acid Residues in Transmembrane Span 7 of the Serotonin Transporter Identified by Random Mutagenesis*
Penado K, Rudnick G, Stephan M. Critical Amino Acid Residues in Transmembrane Span 7 of the Serotonin Transporter Identified by Random Mutagenesis*. Journal Of Biological Chemistry 1998, 273: 28098-28106. PMID: 9774428, DOI: 10.1074/jbc.273.43.28098.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBiological TransportCarrier ProteinsMembrane GlycoproteinsMembrane Transport ProteinsModels, MolecularMolecular Sequence DataMutagenesisNerve Tissue ProteinsProtein ConformationRatsSerotoninSerotonin Plasma Membrane Transport ProteinsStructure-Activity RelationshipConceptsAmino acid residuesRandom mutagenesisAcid residuesTransport activityCritical amino acid residuesRat brain serotonin transporterCritical residuesTransport cycleWild typeNonconservative mutationsStructural predictionsTransporter functionLater stepsMutationsSerotonin transporterResiduesMutagenesisHydrophobic substitutionsTyr-385TransportersMutantsActivitySubstitutionNearby positions
1978
Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles.
Rudnick G, Nelson P. Reconstitution of 5-hydroxytryptamine transport from cholate-disrupted platelet plasma membrane vesicles. Biochemistry 1978, 17: 5300-3. PMID: 728400, DOI: 10.1021/bi00617a033.Peer-Reviewed Original ResearchConceptsPlatelet plasma membrane vesiclesPlasma membrane vesiclesMembrane vesiclesTransport activityNative membrane vesiclesHigh molecular weight aggregatesInhibitor sensitivityVesicular structuresVesiclesIntact plateletsWeight aggregatesIonic requirementsSoybean phospholipidsProteoliposomesTransportersActivityPhospholipidsReconstitution