2017
Calreticulin inhibits inflammation‐induced osteoclastogenesis and bone resorption
Fischer CR, Mikami M, Minematsu H, Nizami S, Lee H, Stamer D, Patel N, Soung D, Back JH, Song L, Drissi H, Lee FY. Calreticulin inhibits inflammation‐induced osteoclastogenesis and bone resorption. Journal Of Orthopaedic Research® 2017, 35: 2658-2666. PMID: 28460421, PMCID: PMC8996436, DOI: 10.1002/jor.23587.Peer-Reviewed Original ResearchConceptsRecombinant human calreticulinIntracellular proteinsCalcium-binding chaperoneDifferent cell typesRecombinant formTranscription factorsFusion of monocytesExtracellular functionsNew therapeutic opportunitiesK activityCathepsin K activityCell typesCalreticulinCytoplasmic 1Anti-osteoclastogenic effectPrecursor cellsMetastatic bone cancerFactor 1Human calreticulinActivated T cellsOsteoclast precursor cellsProteinNuclear factorC-fosMouse calvarial bones
2014
Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)
Carrisoza-Gaytan R, Liu Y, Flores D, Else C, Lee HG, Rhodes G, Sandoval RM, Kleyman TR, Lee FY, Molitoris B, Satlin LM, Rohatgi R. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). American Journal Of Physiology. Renal Physiology 2014, 307: f195-f204. PMID: 24872319, PMCID: PMC4152160, DOI: 10.1152/ajprenal.00634.2013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutocrine CommunicationCell LineDinoprostoneEnzyme ActivationExtracellular Signal-Regulated MAP KinasesFurosemideInjectionsIon TransportKidney CortexKidney Tubules, CollectingMechanotransduction, CellularMiceMicroscopy, Fluorescence, MultiphotonP38 Mitogen-Activated Protein KinasesParacrine CommunicationPhosphorylationPotassiumRatsRats, Sprague-DawleySodium ChlorideStress, MechanicalTime FactorsConceptsTubular fluid flow rateP-p38Biomechanical forcesCircumferential stretchIntravital microscopic approachP-ERK expressionFurosemide injectionPGE2 releaseIntracellular MAPKK secretionCollagen type IVCell culture modelTubular diameterEx vivoPGE2Fluid shear stressUnstretched controlsCCD cellsType IVP38 activationCulture modelMAPK signalingVivoPhysiological forcesCation transport
2012
Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells
Lee HG, Hsu A, Goto H, Nizami S, Lee JH, Cadet ER, Tang P, Shaji R, Chandhanayinyong C, Kweon SH, Oh DS, Tawfeek H, Lee FY. Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells. American Journal Of Physiology - Cell Physiology 2012, 304: c431-c439. PMID: 23255578, PMCID: PMC3602649, DOI: 10.1152/ajpcell.00202.2012.Peer-Reviewed Original ResearchConceptsBone-implant junctionEffect of particlesAbsence of TiWear particlesShear stressMechanical loadingTitanium particlesMetal implantsMechanical perturbationsMechanical strainFluid pressureParticlesBone tissueOsseous integrationTiOsteoprogenitor cellsImplant failureLoadingGene expressionCombined effectMechanical stimuliInterface
2009
Targeting extracellular signal-regulated kinase (ERK) signaling has therapeutic implications for inflammatory osteolysis
Seo SW, Lee D, Minematsu H, Kim AD, Shin M, Cho SK, Kim DW, Yang J, Lee FY. Targeting extracellular signal-regulated kinase (ERK) signaling has therapeutic implications for inflammatory osteolysis. Bone 2009, 46: 695-702. PMID: 19895919, PMCID: PMC2823832, DOI: 10.1016/j.bone.2009.10.032.Peer-Reviewed Original ResearchConceptsInflammatory osteolysisInflammatory responseInnate immune responseExtracellular signal-regulated kinase (ERK) signalingImportant therapeutic targetM-CSF expressionSignal-regulated kinase 1/2 pathwaySignal-regulated kinase signalingExtracellular signal-regulated kinase 1/2 (ERK1/2) pathwayInhibition of ERKImmune responseTherapeutic implicationsTherapeutic targetOsteoclast precursorsKinase 1/2 pathwayM-CSFOsteolysisERK pathwayMacrophage colonyLPSERKERK signalsMitogen-activated protein kinase (MAPK) familyKinase signalingCell differentiation
2007
Nuclear Factor of Activated T Cell Mediates Proinflammatory Gene Expression in Response to Mechanotransduction
AYDEMIR A, LEE S, KIM D, GARDNER TR, PRINCE D, AHN J, LEE F. Nuclear Factor of Activated T Cell Mediates Proinflammatory Gene Expression in Response to Mechanotransduction. Annals Of The New York Academy Of Sciences 2007, 1117: 138-142. PMID: 17584983, DOI: 10.1196/annals.1402.004.Peer-Reviewed Original ResearchOrthopedic Implant Particle‐Induced Tumor Necrosis Factor‐α Production in Macrophage–Monocyte Lineage Cells Is Mediated by Nuclear Factor of Activated T Cells
MINEMATSU H, SHIN MJ, AYDEMIR A, SEO SW, KIM DW, BLAINE TA, MACIÁN F, YANG J, LEE F. Orthopedic Implant Particle‐Induced Tumor Necrosis Factor‐α Production in Macrophage–Monocyte Lineage Cells Is Mediated by Nuclear Factor of Activated T Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 143-150. PMID: 18056040, DOI: 10.1196/annals.1402.026.Peer-Reviewed Original ResearchConceptsActivated T cellsT cellsLineage cellsTumor Necrosis Factor-α ProductionNuclear factorMonocyte-macrophage lineage cellsTNF-alpha protein secretionTNF-alpha expressionTNF-alpha productionTNF-alpha gene expressionTumor necrosis factorMurine macrophage-like RAW264.7Macrophage-like RAW264.7TNF-alpha inductionMonocyte-macrophage cellsTNF-alphaNecrosis factorSpecific molecular mechanismsImplant particlesJoint prosthesesMolecular mechanisms
2005
μ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*
Lee FY, Kim DW, Karmin JA, Hong D, Chang SS, Fujisawa M, Takayanagi H, Bigliani LU, Blaine TA, Lee HJ. μ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*. Journal Of Biological Chemistry 2005, 280: 29929-29936. PMID: 15955824, DOI: 10.1074/jbc.m414600200.Peer-Reviewed Original ResearchConceptsRAW 264.7 cellsNF-kappaB activationReceptor activatorCalpain inhibitorsCell-permeable calpain inhibitorMatrix metalloproteinase-9Regulation of RANKLNF-κB activationNF-κB ligandNF-kappaB ligandMonocyte/macrophage progenitorsRole of calpainMurine RAW 264.7 cellsCell typesMetalloproteinase-9Osteoclastogenic markersCalpain activationResistant acidDecreased expressionOsteoclastogenesisRANKLCalpain activityMu-calpainInhibitorsActivation