2014
Proteomic identification of 14‐3‐3ϵ as a linker protein between pERK1/2 inhibition and BIM upregulation in human osteosarcoma cells
Kim KO, Hsu AC, Lee HG, Patel N, Chandhanayingyong C, Hickernell T, Lee F. Proteomic identification of 14‐3‐3ϵ as a linker protein between pERK1/2 inhibition and BIM upregulation in human osteosarcoma cells. Journal Of Orthopaedic Research® 2014, 32: 848-854. PMID: 24536031, DOI: 10.1002/jor.22598.Peer-Reviewed Original ResearchConceptsPERK1/2 inhibitionHuman osteosarcoma cellsBim expressionSubset of patientsOsteosarcoma cellsConventional cytotoxic agentsConventional cytotoxic treatmentAnti-cancer effectsInhibition of pERK1/2Pro-apoptotic mechanismsMultifocal osteosarcomaMultimodality chemotherapyCytotoxic treatmentUpregulation of BimOsteosarcoma treatmentOsteosarcoma cell deathCytotoxic agentsExpression of BimPotential mediatorsTreatment of PD98059UpregulationTreatmentPD98059 treatmentInhibitionCell death
2011
Targeting Inflammatory Kinase as an Adjuvant Treatment for Osteosarcomas
Noh K, Kim KO, Patel NR, Staples JR, Minematsu H, Nair K, Lee FY. Targeting Inflammatory Kinase as an Adjuvant Treatment for Osteosarcomas. Journal Of Bone And Joint Surgery 2011, 93: 723-732. PMID: 21508279, PMCID: PMC6882535, DOI: 10.2106/jbjs.j.00302.Peer-Reviewed Original ResearchConceptsOsteosarcoma-bearing miceDoxorubicin treatmentVehicle-treated control miceVehicle-treated miceSubset of patientsConventional cytotoxic chemotherapyCell deathOsteosarcoma cellsAnti-cancer effectsAnti-apoptotic protein expressionSignal-regulated kinase 1/2Bcl-2-mediated resistanceInflammatory kinasesAdjuvant treatmentCytotoxic chemotherapyUntreated miceControl miceTreatment outcomesTherapeutic effectERK pathway inhibitorAnti-apoptotic proteinsNude miceAnimal morbidityAggressive osteosarcomaOsteosarcoma cell death
2009
siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells
Kim DW, Kim KO, Shin MJ, Ha JH, Seo SW, Yang J, Lee FY. siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells. Molecular Cancer 2009, 8: 28. PMID: 19445670, PMCID: PMC2689171, DOI: 10.1186/1476-4598-8-28.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsApoptosisATP Binding Cassette Transporter, Subfamily B, Member 1Bcl-X ProteinBone NeoplasmsCell Line, TumorChondrosarcomaDoxorubicinDrug Resistance, NeoplasmFlow CytometryGene SilencingGenes, bcl-2HumansImmunoblottingReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingX-Linked Inhibitor of Apoptosis ProteinConceptsAntiapoptotic proteinsChondrosarcoma cellsAntiapoptotic genesPharmacologic inhibitorsCell survival proteinsChondrosarcoma cell linesSurvival proteinsP-glycoproteinSiRNA knockdownCell typesProteinMechanisms of chemoresistanceCell linesGenesCommon mechanismChemoresistanceSiRNACellsP-glycoprotein inhibitionTherapeutic strategiesInhibitorsKnockdownApoptosisJJ012ConclusionThese findings
2007
Targeting of cell survival genes using small interfering RNAs (siRNAs) enhances radiosensitivity of Grade II chondrosarcoma cells
Kim DW, Seo SW, Cho SK, Chang SS, Lee HW, Lee E, Block JA, Hei TK, Lee FY. Targeting of cell survival genes using small interfering RNAs (siRNAs) enhances radiosensitivity of Grade II chondrosarcoma cells. Journal Of Orthopaedic Research® 2007, 25: 820-828. PMID: 17343283, DOI: 10.1002/jor.20377.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisApoptosis Regulatory ProteinsBcl-X ProteinBone NeoplasmsCell Line, TumorCell SurvivalChondrocytesChondrosarcomaGene Expression Regulation, NeoplasticHumansProto-Oncogene Proteins c-bcl-2Radiation ToleranceRNA, MessengerRNA, Small InterferingX-Linked Inhibitor of Apoptosis ProteinConceptsBcl-xLCell deathChondrosarcoma cellsGene silencingBcl-2Cell survival genesHuman chondrosarcoma cell lineClonogenic survival assaysChondrosarcoma cell linesNovel therapeutic conceptsCorresponding siRNAAntiapoptotic genesApoptotic pathwaySurvival genesAntiapoptotic mRNAsCell survivalXIAP geneMost chondrosarcomasAdjuvant therapySurgical resectionGenesSurvival assaysTherapeutic conceptsCell linesSilencing
2005
Zoledronate induces apoptosis in cells from fibro‐cellular membrane of unicameral bone cyst (UBC)
Yu J, Chang S, Suratwala S, Chung W, Abdelmessieh P, Lee H, Yang J, Lee F. Zoledronate induces apoptosis in cells from fibro‐cellular membrane of unicameral bone cyst (UBC). Journal Of Orthopaedic Research® 2005, 23: 1004-1012. PMID: 15921873, DOI: 10.1016/j.orthres.2005.02.012.Peer-Reviewed Original ResearchConceptsUnicameral bone cystsPeripheral blood mononuclear cellsBlood mononuclear cellsTrabecular bone cellsBone cystUBC cellsMononuclear cellsBone cellsZoledronate concentrationsIsolated peripheral blood mononuclear cellsImmunohisto/cytochemistryPercutaneous injection therapyAnti-resorptive agentsAnti-osteoclastogenic effectApoptotic fractionSTRO-1Potential therapeutic effectsPotential therapeutic roleEffect of zoledronateBenign cystic lesionsNitrogen-containing bisphosphonatesDose-dependent mannerOsteoclast-like cellsInjection therapyPathologic fracture
2004
Bisphosphonates May Reduce Recurrence in Giant Cell Tumor by Inducing Apoptosis
Chang SS, Suratwala SJ, Jung KM, Doppelt JD, Zhang HZ, Blaine TA, Kim TW, Winchester RJ, Lee FY. Bisphosphonates May Reduce Recurrence in Giant Cell Tumor by Inducing Apoptosis. Clinical Orthopaedics And Related Research® 2004, 426: 103-109. PMID: 15346059, DOI: 10.1097/01.blo.0000141372.54456.80.Peer-Reviewed Original ResearchConceptsGiant cell tumorCell tumorsAdjuvant therapyGiant cellsExogenous receptor activatorSpecific adjuvant therapyExtensive bone destructionTumor culturesLocal adjuvant therapyHigh recurrence rateNovel adjuvant therapyOsteoclastlike giant cellsNovel therapeutic agentsNF-kappaB ligandDose-dependent mannerBone destructionRecurrence rateAggressive tumorsBone resorptionSystemic useReceptor activatorAutocrine loopAutocrine mannerTumorsAutocrine regulation
1999
Letters to the editors
Oni O, Lee F, Choi Y, Beherens F, DeFouw D, Einhorn T. Letters to the editors. Journal Of Orthopaedic Research® 1999, 17: 149-150. PMID: 10073659, DOI: 10.1002/jor.1100170121.Peer-Reviewed Original Research
1998
Programmed removal of chondrocytes during endochondral fracture healing
Lee F, Choi Y, Behrens F, DeFouw D, Einhorn T. Programmed removal of chondrocytes during endochondral fracture healing. Journal Of Orthopaedic Research® 1998, 16: 144-150. PMID: 9565087, DOI: 10.1002/jor.1100160124.Peer-Reviewed Original Research