1990
Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function
Eva C, Bovolin P, Balzac F, Botta C, Ricci Gamalero S, Vaccarino F. Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function. Journal Of Molecular Neuroscience 1990, 2: 143. PMID: 2177349, DOI: 10.1007/bf02896839.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylyl CyclasesAnimalsAnimals, NewbornCarbacholCells, CulturedCerebral CortexCholine O-AcetyltransferaseCorpus StriatumKineticsNeuronsPhosphatidylinositolsPolymerase Chain ReactionQuinuclidinyl BenzilateRadioligand AssayRatsReceptors, MuscarinicRNA, MessengerSecond Messenger SystemsConceptsAmount of acetylcholineDensity of mAChRsAdult rat cortexCholinergic receptorsRat cortexNeuronal culturesCholine acetyltransferase-immunoreactive neuronsPrimary culturesMuscarinic cholinergic receptorsTranscriptase-polymerase chain reaction techniqueReverse transcriptase-polymerase chain reaction techniqueNewborn rat brainAdenylate cyclase activityCorticostriatal cellsCorticostriatal neuronsImmunoreactive neuronsSubtype mRNAsChain reaction techniqueMuscarinic antagonistCholinergic transmissionMuscarinic agonistsSecond messenger systemsPostsynaptic componentsNewborn ratsRat brain
1986
Modulation of gamma-aminobutyric acid-mediated inhibitory synaptic currents in dissociated cortical cell cultures.
Vicini S, Alho H, Costa E, Mienville JM, Santi MR, Vaccarino FM. Modulation of gamma-aminobutyric acid-mediated inhibitory synaptic currents in dissociated cortical cell cultures. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 9269-9273. PMID: 3097650, PMCID: PMC387117, DOI: 10.1073/pnas.83.23.9269.Peer-Reviewed Original ResearchConceptsInhibitory synaptic currentsSynaptic currentsRo 15Whole-cell patch-clamp techniqueActions of flunitrazepamGamma-aminobutyric acid receptorsNeonatal rat cortexCortical cell culturesPatch-clamp techniqueImidazobenzodiazepine Ro 15High-affinity ligandsRat cortexSynaptic transmissionPostsynaptic cellElectrical stimulationEndogenous ligandAcid receptorsImmunocytochemical stainingPrimary culturesNeuronsFlunitrazepamCell culturesIntrinsic activityDerivative methylAllosteric regulatory site