Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry
Caron E, Roncagalli R, Hase T, Wolski W, Choi M, Menoita M, Durand S, García-Blesa A, Fierro-Monti I, Sajic T, Heusel M, Weiss T, Malissen M, Schlapbach R, Collins B, Ghosh S, Kitano H, Aebersold R, Malissen B, Gstaiger M. Precise Temporal Profiling of Signaling Complexes in Primary Cells Using SWATH Mass Spectrometry. Cell Reports 2017, 18: 3219-3226. PMID: 28355572, PMCID: PMC5382234, DOI: 10.1016/j.celrep.2017.03.019.Peer-Reviewed Original ResearchConceptsSWATH mass spectrometryProtein interactionsProtein interaction dynamicsPrimary cellsTissue-specific contextDifferential protein expressionCell-signaling eventsInteraction proteomeProtein Grb2Cellular functionsMouse geneticsCell signalingAffinity purificationMammalian tissuesDevelopmental stagesTemporal profilingSpatiotemporal organizationMass spectrometryFundamental processesProtein expressionCell populationsRobust workflowCellsInteraction dynamicsGrb2