2008
A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells
Bonafé N, Rininger JA, Chubet RG, Foellmer HG, Fader S, Anderson JF, Bushmich SL, Anthony K, Ledizet M, Fikrig E, Koski RA, Kaplan P. A recombinant West Nile virus envelope protein vaccine candidate produced in Spodoptera frugiperda expresSF+ cells. Vaccine 2008, 27: 213-222. PMID: 18996430, PMCID: PMC2651515, DOI: 10.1016/j.vaccine.2008.10.046.Peer-Reviewed Original ResearchConceptsVaccine candidatesClinical adverse effectsProtein vaccine candidateRecombinant vaccine candidateWNV vaccineAntibody titersHumoral immunityProtective efficacySafe vaccineWNV infectionChallenge modelVaccine antigensAnimal modelsViral protectionHigh dosesProtein antigensAdverse effectsImmunogenicitySerum-free cultureAntigenCell linesNaïve foalsVaccineAluminum hydroxideInfection
2005
Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens
Magnarelli L, Fikrig E. Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens. Research In Veterinary Science 2005, 79: 99-103. PMID: 15924926, DOI: 10.1016/j.rvsc.2004.11.009.Peer-Reviewed Original Research
2001
Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Oliver JH, Fikrig E. Reactivity of serum samples of dogs and horses tested by use of class-specific recombinant-based enzyme-linked immunosorbent assays for detection of granulocytic ehrlichiosis. American Journal Of Veterinary Research 2001, 62: 1365-9. PMID: 11560261, DOI: 10.2460/ajvr.2001.62.1365.Peer-Reviewed Original ResearchConceptsIgG antibodiesSerum samplesHGE agentGranulocytic ehrlichiosisClient-owned dogsDog serum samplesHuman granulocytic ehrlichiosis (HGE) agentEnzyme-linked immunosorbentPositive resultsWestern blot analysisTick-infested areasEhrlichia phagocytophila genogroupTotal immunoglobulinHorse serum samplesPromyelocytic leukemia cellsClinical relevanceHuman promyelocytic leukemia cellsProtein antigensIgMELISADogsLeukemia cellsAntibodiesBlot analysisAntigenEvaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Fikrig E. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. American Journal Of Veterinary Research 2001, 62: 29-32. PMID: 11197555, DOI: 10.2460/ajvr.2001.62.29.Peer-Reviewed Original ResearchConceptsEquine samplesPolyvalent ELISAPolyvalent enzyme-linked immunosorbentGranulocytic ehrlichiosisIFA stainingEquine serum samplesE risticiiEhrlichia canisCanine serum samplesCanine samplesSpecific recombinant antigensE equiHorsesEhrlichiosisPercentage of samplesIndirect fluorescent antibody stainingRecombinant antigensDogsWhole cell antigenCanisEquiELISA resultsSerum samplesPresent studyFluorescent antibody staining
2000
Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Padula SJ, Fikrig E. Serologic confirmation of Ehrlichia equi and Borrelia burgdorferi infections in horses from the northeastern United States. Journal Of The American Veterinary Medical Association 2000, 217: 1045-50. PMID: 11019714, DOI: 10.2460/javma.2000.217.1045.Peer-Reviewed Original ResearchConceptsB burgdorferiClinical signsGranulocytic ehrlichiosisSerum samplesDetectable serum antibodiesImmunoblot analysisIndirect fluorescent antibody stainingBorrelial antibodiesTick-infested areasRecombinant protein antigensProspective studyDetectable antibodiesSerum antibodiesSerologic confirmationFluorescent antibody stainingE equiAntibody reactivityClinical relevanceEtiologic agentClinical diagnosisEqui antibodiesProtein antigensEhrlichia equiELISAAntibodies
1999
Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis.
Magnarelli L, Van Andel A, Ijdo J, Heimer R, Fikrig E. Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis. American Journal Of Veterinary Research 1999, 60: 631-5. PMID: 10328436, DOI: 10.2460/ajvr.1999.60.05.631.Peer-Reviewed Original Research
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatients