2007
A West Nile Virus Recombinant Protein Vaccine That Coactivates Innate and Adaptive Immunity
Huleatt J, Foellmer H, Hewitt D, Tang J, Desai P, Price A, Jacobs A, Takahashi V, Huang Y, Nakaar V, Alexopoulou L, Fikrig E, Powell T, McDonald W. A West Nile Virus Recombinant Protein Vaccine That Coactivates Innate and Adaptive Immunity. The Journal Of Infectious Diseases 2007, 195: 1607-1617. PMID: 17471430, DOI: 10.1086/517613.Peer-Reviewed Original ResearchConceptsImmune responseAntibody responseImmunoglobulin G antibody responseC3H/HeN miceWest Nile virus vaccineAdaptive immune signalsTLR5-deficient miceAntigen-specific responsesG antibody responseProtective immune responseAdaptive immune responsesToll-like receptorsWNV envelope proteinLethal WNV challengeRecombinant protein vaccineInterleukin-8 productionNeutralized viral infectivityEnzyme-linked immunosorbentEffective WNV vaccinesWNV vaccineHeN miceVirus challengeProtein vaccineVirus vaccineWNV challenge
1997
Protective and Arthritis-Resolving Activity in Sera of Mice Infected with Borrelia burgdorferi
Barthold S, Feng S, Bockenstedt L, Fikrig E, Feen K. Protective and Arthritis-Resolving Activity in Sera of Mice Infected with Borrelia burgdorferi. Clinical Infectious Diseases 1997, 25: s9-s17. PMID: 9233658, DOI: 10.1086/516166.Peer-Reviewed Original ResearchConceptsImmune serumSyringe challengePassive protective activitySerum of miceArthritis resolutionHost-adapted spirochetesImmunocompetent miceJoint tissuesTissue transplantsProtective activityImmunodominant regionMiceSerumInfectionBorrelia burgdorferiB. burgdorferiRecombinant formBurgdorferiSpirochetesTissueChallenge inoculationCarditisTransplantSpirochetemiaAntigenLyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis
Fikrig E, Barthold S. Lyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis. FEMS Microbiology Letters 1997, 148: 137-143. PMID: 9084140, DOI: 10.1111/j.1574-6968.1997.tb10279.x.Peer-Reviewed Original ResearchConceptsTransgenic miceAntibody responseNervous systemNon-transgenic miceFlagellin epitopeTypes of miceB. burgdorferi infectionCentral nervous systemMyelin basic proteinBurgdorferi infectionNeurologic diseaseMyelinated regionsNeuroborreliosisLyme diseaseMiceNeural tissueBorrelia burgdorferiEpitopesFlagellin antibodyInfectionDiseaseBasic proteinCarditisArthritisFusion proteinTemporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.
Das S, Barthold SW, Giles SS, Montgomery RR, Telford SR, Fikrig E. Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host. Journal Of Clinical Investigation 1997, 99: 987-995. PMID: 9062357, PMCID: PMC507907, DOI: 10.1172/jci119264.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupElectrophoresis, Gel, Pulsed-FieldEnzyme-Linked Immunosorbent AssayFemaleFlagellinFluorescent Antibody Technique, IndirectGene Expression Regulation, BacterialHumansImmunizationImmunization, PassiveImmunoblottingIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HPlasmidsPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerTime FactorsConceptsInfected miceHumoral responseLate-stage Lyme diseaseMarkers of infectionCourse of diseaseMurine Lyme borreliosisB. burgdorferiB. burgdorferi infectionHuman humoral responseIxodes dammini ticksBurgdorferi-infected miceLyme arthritisActive immunizationMammalian hostsPassive transferBurgdorferi infectionC3H miceMurine infectionDay 14P21 antibodyP21 expressionLyme borreliosisLyme diseaseMiceInfection
1996
Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis
Magnarelli LA, Fikrig E, Padula SJ, Anderson JF, Flavell RA. Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis. Journal Of Clinical Microbiology 1996, 34: 237-240. PMID: 8788993, PMCID: PMC228775, DOI: 10.1128/jcm.34.2.237-240.1996.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assaySerum samplesRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysLyme borreliosisClass-specific enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiImmunoglobulin M antibodiesB. burgdorferi infectionOspC antigenErythema migransRheumatoid arthritisM antibodiesBorrelia burgdorferi sensu strictoIgG antibodiesOral infectionSerologic testsBurgdorferi infectionBurgdorferi sensu strictoPredominant reactivityAntigenOuter surface proteins OspAImmunosorbent assayBorrelia burgdorferiB. burgdorferi
1995
Evaluation of a lyme disease enzyme immunoassay using the 41-G fragment of flagellin
Cretella S, Gordon S, Flavell RA, Fikrig E. Evaluation of a lyme disease enzyme immunoassay using the 41-G fragment of flagellin. European Journal Of Clinical Microbiology & Infectious Diseases 1995, 14: 609-613. PMID: 7588849, DOI: 10.1007/bf01690736.Peer-Reviewed Original ResearchREACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI
Magnarelli L, Anderson J, Flavell R, Fikrig E. REACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI. Journal Of Wildlife Diseases 1995, 31: 339-344. PMID: 8592354, DOI: 10.7589/0090-3558-31.3.339.Peer-Reviewed Original ResearchConceptsWhole cell antigenCell antigensRecombinant antigensSpecific recombinant antigensGeometric mean antibody titersMean antibody titersPresence of antibodiesB. burgdorferi infectionEnzyme-linked immunosorbentWhite-footed miceAntibody titersBorrelia burgdorferi sensu strictoBurgdorferi infectionBurgdorferi sensu stricto
1993
Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi
Fikrig E, Magnarelli LA, Chen M, Anderson JF, Flavell RA. Serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of Borrelia burgdorferi. Journal Of Clinical Microbiology 1993, 31: 2451-2455. PMID: 7691874, PMCID: PMC265777, DOI: 10.1128/jcm.31.9.2451-2455.1993.Peer-Reviewed Original ResearchSerologic response to the Borrelia burgdorferi flagellin demonstrates an epitope common to a neuroblastoma cell line.
Fikrig E, Berland R, Chen M, Williams S, Sigal LH, Flavell RA. Serologic response to the Borrelia burgdorferi flagellin demonstrates an epitope common to a neuroblastoma cell line. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 183-187. PMID: 7678336, PMCID: PMC45624, DOI: 10.1073/pnas.90.1.183.Peer-Reviewed Original ResearchConceptsNeuroblastoma cell linesHuman neuroblastoma cell linePatient seraCell linesPathogenesis of neuroborreliosisNeural tissueB. burgdorferi flagellinB-cell epitopesAmino acids 213Neurologic manifestationsSerologic responseSpecific B-cell epitopesBorrelia burgdorferi flagellinImmune responseLyme borreliosisMonoclonal antibodiesRecombinant fusion proteinSerumAntibodiesBorrelia burgdorferiEpitopesEpitope mappingFlagellinTissueNeuroborreliosis
1992
Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis
Magnarelli LA, Fikrig E, Berland R, Anderson JF, Flavell RA. Comparison of whole-cell antibodies and an antigenic flagellar epitope of Borrelia burgdorferi in serologic tests for diagnosis of Lyme borreliosis. Journal Of Clinical Microbiology 1992, 30: 3158-3162. PMID: 1280650, PMCID: PMC270607, DOI: 10.1128/jcm.30.12.3158-3162.1992.Peer-Reviewed Original ResearchConceptsWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayLyme borreliosisB. burgdorferiRecombinant flagellar proteinsBorrelia burgdorferiSecond study groupFlagellar epitopesErythema migransDetectable antibodiesSerologic testsTreponemal antibodiesRecombinant proteinsStudy groupImmunosorbent assayAntibodiesAntigenCausative agentAntigenic regionsBorreliosisBurgdorferiComparable sensitivityHuman serumMigransSerumSerologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and Band Flagellin
Fikrig E, Huguenel E, Berland R, Rahn D, Hardin J, Flavell R. Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and Band Flagellin. The Journal Of Infectious Diseases 1992, 165: 1127-1132. PMID: 1583333, DOI: 10.1093/infdis/165.6.1127.Peer-Reviewed Original ResearchConceptsLyme diseaseRecombinant outer surface proteinRecombinant outer surface protein AB. burgdorferi antigensSurface protein AOuter surface protein AEarly diseaseLate diseaseSerologic diagnosisDiagnostic testingOuter surface proteinsImmunogenic regionPatientsDiseaseBorrelia burgdorferiELISAImmunoblotAntibodiesSurface proteinsFlagellinProtein AAntigenDiagnosisRoles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice
Fikrig E, Barthold SW, Marcantonio N, Deponte K, Kantor FS, Flavell RA. Roles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice. Infection And Immunity 1992, 60: 657-661. PMID: 1730500, PMCID: PMC257680, DOI: 10.1128/iai.60.2.657-661.1992.Peer-Reviewed Original ResearchConceptsProtective immunityProtective effectRecombinant outer surface protein AActive immunization experimentsSurface protein AOuter surface protein AB. burgdorferi proteinsActive immunizationResidual seraRecombinant OspAImmunization experimentsLyme borreliosisInfectionLyme disease agentMiceImmunityBorrelia burgdorferiAntibodiesImmunizationOspAOspBLaboratory miceSerumFlagellinDisease agents
1991
Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent
Berland R, Fikrig E, Rahn D, Hardin J, Flavell RA. Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent. Infection And Immunity 1991, 59: 3531-3535. PMID: 1894359, PMCID: PMC258917, DOI: 10.1128/iai.59.10.3531-3535.1991.Peer-Reviewed Original ResearchConceptsLyme diseaseHumoral responseLate-stage Lyme diseaseEarly humoral responseBorrelia burgdorferiFlagellar antigensPolymerase chain reactionSerological testingImmunodominant domainWestern blotPatientsLyme disease agentAntigenDiseaseChain reactionGlutathione S-transferaseFusion proteinAffinity-purified fusion proteinFlagellin fragmentBacterial flagellinSerumBurgdorferiS-transferaseDisease agentsMolecular characterization