2017
Multiple UBXN family members inhibit retrovirus and lentivirus production and canonical NFκΒ signaling by stabilizing IκBα
Hu Y, O’Boyle K, Auer J, Raju S, You F, Wang P, Fikrig E, Sutton RE. Multiple UBXN family members inhibit retrovirus and lentivirus production and canonical NFκΒ signaling by stabilizing IκBα. PLOS Pathogens 2017, 13: e1006187. PMID: 28152074, PMCID: PMC5308826, DOI: 10.1371/journal.ppat.1006187.Peer-Reviewed Original ResearchConceptsGene expressionHundreds of genesCycle assaysNFκB pathwayCo-immunoprecipitation studiesMouse embryo fibroblastsDownstream effector functionsJurkat T cellsPrimary human fibroblastsEmbryonic lethalityFamily membersUBA domainUBXN1HIV gene expressionSingle-cycle assaysProtein turnoverEmbryo fibroblastsCell adhesionGlobal regulationCanonical NFκB pathwayNFκB signalingHuman fibroblastsLike receptorsPathwayKnockdown
2014
A Tick Gut Protein with Fibronectin III Domains Aids Borrelia burgdorferi Congregation to the Gut during Transmission
Narasimhan S, Coumou J, Schuijt TJ, Boder E, Hovius JW, Fikrig E. A Tick Gut Protein with Fibronectin III Domains Aids Borrelia burgdorferi Congregation to the Gut during Transmission. PLOS Pathogens 2014, 10: e1004278. PMID: 25102051, PMCID: PMC4125277, DOI: 10.1371/journal.ppat.1004278.Peer-Reviewed Original ResearchInnexin AGAP001476 Is Critical for Mediating Anti-Plasmodium Responses in Anopheles Mosquitoes
Li MW, Wang J, Zhao YO, Fikrig E. Innexin AGAP001476 Is Critical for Mediating Anti-Plasmodium Responses in Anopheles Mosquitoes. Journal Of Biological Chemistry 2014, 289: 24885-24897. PMID: 25035430, PMCID: PMC4155657, DOI: 10.1074/jbc.m114.554519.Peer-Reviewed Original ResearchConceptsAnti-Plasmodium responseToll pathwayInnate immune responseGap junction proteinIMD pathwaySignal transductionExtracellular communicationSimultaneous knockdownAnopheles gambiaeImmune responseMidgut epitheliumInhibitor studiesKnockdownPlasmodium falciparum infectionCactusBlood mealGap junctionsVitellogenin levelsOocyst numbersAnopheles mosquitoesJunction proteinsCritical rolePathwayMRNA levelsMosquitoes
2013
ELF4 is critical for induction of type I interferon and the host antiviral response
You F, Wang P, Yang L, Yang G, Zhao YO, Qian F, Walker W, Sutton R, Montgomery R, Lin R, Iwasaki A, Fikrig E. ELF4 is critical for induction of type I interferon and the host antiviral response. Nature Immunology 2013, 14: 1237-1246. PMID: 24185615, PMCID: PMC3939855, DOI: 10.1038/ni.2756.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCells, CulturedDNA-Binding ProteinsHEK293 CellsHeLa CellsHost-Pathogen InteractionsHumansImmunoblottingInterferon Regulatory Factor-3Interferon Regulatory Factor-7Interferon-betaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalProtein BindingReverse Transcriptase Polymerase Chain ReactionRNA InterferenceSignal TransductionSurvival AnalysisTranscription FactorsTranscriptional ActivationWest Nile FeverWest Nile virus
2004
Disruption of Ixodes scapularis anticoagulation by using RNA interference
Narasimhan S, Montgomery RR, DePonte K, Tschudi C, Marcantonio N, Anderson JF, Sauer JR, Cappello M, Kantor FS, Fikrig E. Disruption of Ixodes scapularis anticoagulation by using RNA interference. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 1141-1146. PMID: 14745044, PMCID: PMC337020, DOI: 10.1073/pnas.0307669100.Peer-Reviewed Original ResearchConceptsAnti-factor Xa activityNumerous infectious diseasesIxodes scapularis ticksRNA interferenceMultiple anticoagulantsReduction of mRNAXa activityVaccine candidatesInfectious diseasesTick salivaAnticoagulantsBabesia microtiPhysiologic functionI. scapularis salivaSalivary glandsBorrelia burgdorferiTick feedingScapularis ticksAnticoagulant activityAnaplasma phagocytophilumTick engorgementSuch interventionsEngorgement weightEngorgementKey targetOspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
Pal U, Yang X, Chen M, Bockenstedt LK, Anderson JF, Flavell RA, Norgard MV, Fikrig E. OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands. Journal Of Clinical Investigation 2004, 113: 220-230. PMID: 14722614, PMCID: PMC311436, DOI: 10.1172/jci19894.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAntigens, BacterialBacterial Outer Membrane ProteinsBorrelia burgdorferiDisease Models, AnimalEnzyme-Linked Immunosorbent AssayGenetic VectorsImmunoglobulin Fab FragmentsIxodesLipoproteinsLyme DiseaseMiceMice, SCIDMicroscopy, ConfocalModels, GeneticPlasmidsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSalivary GlandsSpirochaetalesTime FactorsUp-RegulationConceptsTick salivary glandsWild-type B. burgdorferiArthropod vectorsWild-type spirochetesTransmission of spirochetesB. burgdorferiMajor surface lipoproteinMammalian hostsGene expressionSalivary glandsOspC geneSurface lipoproteinsSalivary gland colonizationInfectious cloneOuter surface protein CGland colonizationSurface protein CTick gutMutantsOspCUnfed ticksInvasionCritical stepBorrelia burgdorferiSpirochetes
1995
Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding.
De Silva AM, Fikrig E. Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding. American Journal Of Tropical Medicine And Hygiene 1995, 53: 397-404. PMID: 7485694, DOI: 10.4269/ajtmh.1995.53.397.Peer-Reviewed Original Research